Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Jun;146(3):1166–1169. doi: 10.1128/jb.146.3.1166-1169.1981

Isolation and genetic characterization of Escherichia coli mutants defective in propionate metabolism.

S K Spratt, C L Ginsburgh, W D Nunn
PMCID: PMC216975  PMID: 7016831

Abstract

Escherichia coli mutants defective in propionate metabolism (Prp-) were isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Prp- mutants demonstrate a phenotypic inability to grow on odd-chain-length fatty acids. The new genetic locus for the Prp- phenotype maps at approximately 98 min on the E. coli chromosome.

Full text

PDF
1169

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Böck A., Faiman L. E., Neidhardt F. C. Biochemical and genetic characterization of a mutant of Escherichia coli with a temperature-sensitive valyl ribonucleic acid synthetase. J Bacteriol. 1966 Oct;92(4):1076–1082. doi: 10.1128/jb.92.4.1076-1082.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CLARK A. J. Genetic analysis of a "double male" strain of Escherichia coli K-12. Genetics. 1963 Jan;48:105–120. doi: 10.1093/genetics/48.1.105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Carl P. L. Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol Gen Genet. 1970;109(2):107–122. doi: 10.1007/BF00269647. [DOI] [PubMed] [Google Scholar]
  6. Chumley F. G., Menzel R., Roth J. R. Hfr formation directed by tn10. Genetics. 1979 Apr;91(4):639–655. doi: 10.1093/genetics/91.4.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kay W. W. Genetic control of the metabolism of propionate by Escherichia coli K12. Biochim Biophys Acta. 1972 May 16;264(3):508–521. doi: 10.1016/0304-4165(72)90014-1. [DOI] [PubMed] [Google Scholar]
  8. Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nunn W. D., Simons R. W., Egan P. A., Maloy S. R. Kinetics of the utilization of medium and long chain fatty acids by mutant of Escherichia coli defective in the fadL gene. J Biol Chem. 1979 Sep 25;254(18):9130–9134. [PubMed] [Google Scholar]
  10. Nunn W. D., Simons R. W. Transport of long-chain fatty acids by Escherichia coli: mapping and characterization of mutants in the fadL gene. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3377–3381. doi: 10.1073/pnas.75.7.3377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Robert-Baudouy J. M., Portalier R. C. Mutations affectant le catabolisme du glucuronate chez Escherichia coli K12. Mol Gen Genet. 1974;131(1):31–46. doi: 10.1007/BF00269385. [DOI] [PubMed] [Google Scholar]
  12. Salanitro J. P., Wegener W. S. Growth of Escherichia coli on short-chain fatty acids: growth characteristics of mutants. J Bacteriol. 1971 Nov;108(2):885–892. doi: 10.1128/jb.108.2.885-892.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Simons R. W., Egan P. A., Chute H. T., Nunn W. D. Regulation of fatty acid degradation in Escherichia coli: isolation and characterization of strains bearing insertion and temperature-sensitive mutations in gene fadR. J Bacteriol. 1980 May;142(2):621–632. doi: 10.1128/jb.142.2.621-632.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taylor A L, Adelberg E A. Linkage Analysis with Very High Frequency Males of Escherichia Coli. Genetics. 1960 Sep;45(9):1233–1243. doi: 10.1093/genetics/45.9.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weeks G., Shapiro M., Burns R. O., Wakil S. J. Control of fatty acid metabolism. I. Induction of the enzymes of fatty acid oxidation in Escherichia coli. J Bacteriol. 1969 Feb;97(2):827–836. doi: 10.1128/jb.97.2.827-836.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wegener W. S., Reeves H. C., Ajl S. J. Propionate metabolism. II. Factors regulating adaptation of Escherichia coli to propionate. Arch Biochem Biophys. 1968 Jan;123(1):55–61. doi: 10.1016/0003-9861(68)90102-1. [DOI] [PubMed] [Google Scholar]
  17. Wegener W. S., Reeves H. C., Ajl S. J. Propionate oxidation in Escherichia coli. Arch Biochem Biophys. 1967 Aug;121(2):440–442. doi: 10.1016/0003-9861(67)90098-7. [DOI] [PubMed] [Google Scholar]
  18. Wegener W. S., Reeves H. C., Rabin R., Ajl S. J. Alternate pathways of metabolism of short-chain fatty acids. Bacteriol Rev. 1968 Mar;32(1):1–26. doi: 10.1128/br.32.1.1-26.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES