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. 1981 Apr;146(1):337–344. doi: 10.1128/jb.146.1.337-344.1981

Induction of citric acid cycle enzymes during initiation of sporulation by guanine nucleotide deprivation.

B Uratani-Wong, J M Lopez, E Freese
PMCID: PMC217088  PMID: 6783618

Abstract

In Bacillus subtilis, conditions causing partial deprivation of guanine nucleotides initiated sporulation and caused the synthesis of citrate synthase, aconitase, and alpha-ketoglutarate dehydrogenase. Alpha-ketoglutarate dehydrogenase could also be induced by acetate, and the specific activity of this enzyme was elevated in mutants that had high intracellular acetyl coenzyme A concentrations because they lacked citrate synthase activity. After deprivation of guanine nucleotides, the intracellular concentration of acetyl coenzyme A also increased, which explained the induction of alpha-ketoglutarate dehydrogenase. Furthermore, the decreases in alpha-ketoglutarate and L-malate concentrations observed during this deprivation accounted for the observed increases in citrate synthase activity (which was repressed by alpha-ketoglutarate and malate) and aconitase activity (which was repressed by alpha-ketoglutarate).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cox D. P., Hanson R. S. Catabolite repression of aconitate hydratase in Bacillus subtilis. Biochim Biophys Acta. 1968 Apr 16;158(1):36–44. doi: 10.1016/0304-4165(68)90069-x. [DOI] [PubMed] [Google Scholar]
  2. Flechtner V. R., Hanson R. S. Coarse and fine control of citrate synthase from Bacillus subtilis. Biochim Biophys Acta. 1969 Jul 30;184(2):252–262. doi: 10.1016/0304-4165(69)90027-0. [DOI] [PubMed] [Google Scholar]
  3. Fortnagel P., Freese E. Analysis of sporulation mutants. II. Mutants blocked in the citric acid cycle. J Bacteriol. 1968 Apr;95(4):1431–1438. doi: 10.1128/jb.95.4.1431-1438.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fortnagel P. The regulation of aconitase and isocitrate dehydrogenase in sporulation mutants of Bacillus subtilis. Biochim Biophys Acta. 1970 Nov 24;222(2):290–298. doi: 10.1016/0304-4165(70)90116-9. [DOI] [PubMed] [Google Scholar]
  5. Freese E. B., Vasantha N., Freese E. Induction of sporulation in developmental mutants of Bacillus subtilis. Mol Gen Genet. 1979 Feb 16;170(1):67–74. doi: 10.1007/BF00268581. [DOI] [PubMed] [Google Scholar]
  6. Freese E., Heinze J. E., Galliers E. M. Partial purine deprivation causes sporulation of Bacillus subtilis in the presence of excess ammonia, glucose and phosphate. J Gen Microbiol. 1979 Nov;115(1):193–205. doi: 10.1099/00221287-115-1-193. [DOI] [PubMed] [Google Scholar]
  7. Hanson R. S., Cox D. P. Effect of different nutritional conditions on the synthesis of tricarboxylic acid cycle enzymes. J Bacteriol. 1967 Jun;93(6):1777–1787. doi: 10.1128/jb.93.6.1777-1787.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heinze J. E., Mitani T., Rich K. E., Freese E. Induction of sporulation by inhibitory purines and related compounds. Biochim Biophys Acta. 1978 Nov 21;521(1):16–26. doi: 10.1016/0005-2787(78)90245-9. [DOI] [PubMed] [Google Scholar]
  9. Kalb V. F., Jr, Bernlohr R. W. A new spectrophotometric assay for protein in cell extracts. Anal Biochem. 1977 Oct;82(2):362–371. doi: 10.1016/0003-2697(77)90173-7. [DOI] [PubMed] [Google Scholar]
  10. Lopez J. M., Marks C. L., Freese E. The decrease of guanine nucleotides initiates sporulation of Bacillus subtilis. Biochim Biophys Acta. 1979 Oct 4;587(2):238–252. doi: 10.1016/0304-4165(79)90357-x. [DOI] [PubMed] [Google Scholar]
  11. Lopez J. M., Uratani-Wong B., Freese E. Catabolite repression of enzyme synthesis does not prevent sporulation. J Bacteriol. 1980 Mar;141(3):1447–1449. doi: 10.1128/jb.141.3.1447-1449.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mitani T., Heinze J. E., Freese E. Induction of sporulation in Bacillus subtilis by decoyinine or hadacidin. Biochem Biophys Res Commun. 1977 Aug 8;77(3):1118–1125. doi: 10.1016/s0006-291x(77)80094-6. [DOI] [PubMed] [Google Scholar]
  13. Ohné M. Regulation of the dicarboxylic acid part of the citric acid cycle in Bacillus subtilis. J Bacteriol. 1975 Apr;122(1):224–234. doi: 10.1128/jb.122.1.224-234.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rutberg B., Hoch J. A. Citric acid cycle: gene-enzyme relationships in Bacillus subtilis. J Bacteriol. 1970 Nov;104(2):826–833. doi: 10.1128/jb.104.2.826-833.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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