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. 1981 Mar;145(3):1421–1424. doi: 10.1128/jb.145.3.1421-1424.1981

Curing of the 2 mu DNA plasmid from Saccharomyces cerevisiae.

A Toh-e, R B Wickner
PMCID: PMC217149  PMID: 7009587

Abstract

The 2 mu DNA plasmid is often eliminated from yeast cells when they are transformed with the 2 mu DNA-LEU2-pMB9 composite plasmid pJDB219. Since pJDB219 is subsequently lost with high frequency, derivatives lacking all 2 mu DNA can be prepared from any strain.

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Selected References

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