Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Jan;145(1):293–298. doi: 10.1128/jb.145.1.293-298.1981

Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome.

A Sarthy, S Michaelis, J Beckwith
PMCID: PMC217272  PMID: 7007316

Abstract

We present genetic evidence which demonstrates that the phoA gene is transcribed in the clockwise direction on the Escherichia coli chromosome, in contrast to an earlier proposal. Our conclusion is based on analysis of various genetic fusions between the lac operon and the phoA gene.

Full text

PDF
294

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B. Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980 Mar;44(1):1–56. doi: 10.1128/mr.44.1.1-56.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bassford P. J., Jr, Silhavy T. J., Beckwith J. R. Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm. J Bacteriol. 1979 Jul;139(1):19–31. doi: 10.1128/jb.139.1.19-31.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Debarbouille M., Schwartz M. The use of gene fusions to study the expression of malT the positive regulator gene of the maltose regulon. J Mol Biol. 1979 Aug 15;132(3):521–534. doi: 10.1016/0022-2836(79)90273-0. [DOI] [PubMed] [Google Scholar]
  4. Nakata A., Peterson G. R., Brooks E. L., Rothman F. G. Location and orientation of the phoA locus on the Escherichia coli K-12 linkage map. J Bacteriol. 1971 Sep;107(3):683–689. doi: 10.1128/jb.107.3.683-689.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Newton W. A., Beckwith J. R., Zipser D., Brenner S. Nonsense mutants and polarity in the lac operon of Escherichia coli. J Mol Biol. 1965 Nov;14(1):290–296. doi: 10.1016/s0022-2836(65)80250-9. [DOI] [PubMed] [Google Scholar]
  6. Sarthy A., Fowler A., Zabin I., Beckwith J. Use of gene fusions to determine a partial signal sequence of alkaline phosphatase. J Bacteriol. 1979 Sep;139(3):932–939. doi: 10.1128/jb.139.3.932-939.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sarthy A., Michaelis S., Beckwith J. Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA. J Bacteriol. 1981 Jan;145(1):288–292. doi: 10.1128/jb.145.1.288-292.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Silhavy T. J., Brickman E., Bassford P. J., Jr, Casadaban M. J., Shuman H. A., Schwartz V., Guarente L., Schwartz M., Beckwith J. R. Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon. Mol Gen Genet. 1979 Jul 24;174(3):249–259. doi: 10.1007/BF00267797. [DOI] [PubMed] [Google Scholar]
  9. Suzuki T., Garen A. Fragments of alkaline phosphatase from nonsense mutants. I. Isolation and characterization of fragments from amber and ochre mutants. J Mol Biol. 1969 Nov 14;45(3):549–566. doi: 10.1016/0022-2836(69)90312-x. [DOI] [PubMed] [Google Scholar]
  10. TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]
  11. Yagil E., Bracha M., Silberstein N. Further genetic mapping of the phoA-phoR region for alkaline phosphatase synthesis in Escherichia coli K 12. Mol Gen Genet. 1970;109(1):18–26. doi: 10.1007/BF00334043. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES