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. 1981 Jan;145(1):358–368. doi: 10.1128/jb.145.1.358-368.1981

Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu.

Y Murooka, N Takizawa, T Harada
PMCID: PMC217280  PMID: 6450749

Abstract

The host range of coliphage Mu was greatly expanded to various genera of gram-negative bacteria by using the hybrid plasmic RP4::Mu cts, which is temperature sensitive and which confers resistance to ampicillin, kanamycin, and tetracycline. These drug resistance genes were transferred from Escherichia coli to members of the general Klebsiella, Enterobacter, Citrobacter, Salmonella, Proteus, Erwinia, Serratia, Alcaligenes, Agrobacterium, Rhizobium, Pseudomonas, Acetobacter, and Bacillus. Mu phage was produced by thermal induction from the lysogens of all these drug-resistant bacteria except Bacillus. Mu phage and RP4 or the RP4::Mu plasmid were used to create intergeneric recombinant strains by transfer of some genes, including the arylsulfatase gene, between Klebsiella aerogenes and E. coli. Thus, genetic analysis and intergeneric gene transfer are possible in these RP4::Mu-sensitive bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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