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. 1983 Jan;153(1):384–389. doi: 10.1128/jb.153.1.384-389.1983

New class of mutations in Escherichia coli (uup) that affect precise excision of insertion elements and bacteriophage Mu growth.

J D Hopkins, M Clements, M Syvanen
PMCID: PMC217384  PMID: 6294054

Abstract

We have used a papillation screening technique to isolate mutations that increase the precise excision of insertion elements. The three mutations isolated stimulated precise excision of Tn5, Tn10, and the IS elements. They had a large, 20- to 600-fold, effect on excision of Tn5 at various chromosomal sites. The varied stimulation for different Tn5 insertions showed that the mutations altered the relationship between a precise excision activity and the chromosomal sequence flanking an inserted Tn5. A much smaller stimulation was observed for insertions on the plasmid F'128. The stimulation was recA independent. The mutations also reduced the rate of production of bacteriophage Mu progeny. The mutations were mapped by two- and three-factor crosses with closely linked Tn10 insertions. They defined the uup locus, located at 21.3 min on the Escherichia coli map, next to pyrD.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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