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. 1983 Jan;153(1):527–531. doi: 10.1128/jb.153.1.527-531.1983

Heat Curing of a Sym Plasmid in a Fast-Growing Rhizobium sp. That Is Able to Nodulate Legumes and the Nonlegume Parasponia sp

Nigel A Morrison 1, Cen Y Hau 1, Michael J Trinick 2, John Shine 1, Barry G Rolfe 1
PMCID: PMC217402  PMID: 6571729

Abstract

Genes involved in nodulation of both legumes and the nonlegume Parasponia sp., as well as nitrogenase genes, reside on a large plasmid in a fast-growing Rhizobium sp. from Lablab purpureus. This plasmid can be cured by incubation at elevated temperatures and can be mobilized by the P1 group plasmid RP1::Tn501.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett P. M., Richmond M. H. Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Eschericha coli. J Bacteriol. 1976 Apr;126(1):1–6. doi: 10.1128/jb.126.1.1-6.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cen Y., Bender G. L., Trinick M. J., Morrison N. A., Scott K. F., Gresshoff P. M., Shine J., Rolfe B. G. Transposon mutagenesis in rhizobia which can nodulate both legumes and the nonlegume parasponia. Appl Environ Microbiol. 1982 Jan;43(1):233–236. doi: 10.1128/aem.43.1.233-236.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eckhardt T. A rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. Plasmid. 1978 Sep;1(4):584–588. doi: 10.1016/0147-619x(78)90016-1. [DOI] [PubMed] [Google Scholar]
  4. FAHRAEUS G. The infection of clover root hairs by nodule bacteria studied by a simple glass slide technique. J Gen Microbiol. 1957 Apr;16(2):374–381. doi: 10.1099/00221287-16-2-374. [DOI] [PubMed] [Google Scholar]
  5. Haas D., Holloway B. W. R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. Mol Gen Genet. 1976 Mar 30;144(3):243–251. doi: 10.1007/BF00341722. [DOI] [PubMed] [Google Scholar]
  6. Pemberton J. M., Bowen A. R. High-frequency chromosome transfer in Rhodopseudomonas sphaeroides promoted by broad-host-range plasmid RP1 carrying mercury transposon Tn501. J Bacteriol. 1981 Jul;147(1):110–117. doi: 10.1128/jb.147.1.110-117.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Scott K. F., Hughes J. E., Gresshoff P. M., Beringer J. E., Rolfe B. G., Shine J. Molecular cloning of Rhizobium trifolii genes involved in symbiotic nitrogen fixation. J Mol Appl Genet. 1982;1(4):315–326. [PubMed] [Google Scholar]
  8. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  9. Taylor J. M., Illmensee R., Summers J. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase. Biochim Biophys Acta. 1976 Sep 6;442(3):324–330. doi: 10.1016/0005-2787(76)90307-5. [DOI] [PubMed] [Google Scholar]
  10. Whitfeld P. L., Seeburg P. H., Shine J. The human pro-opiomelanocortin gene: organization, sequence, and interspersion with repetitive DNA. DNA. 1982;1(2):133–143. doi: 10.1089/dna.1.1982.1.133. [DOI] [PubMed] [Google Scholar]

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