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. 1983 May;154(2):708–718. doi: 10.1128/jb.154.2.708-718.1983

Membrane cytochromes of Escherichia coli grown aerobically and anaerobically with nitrate.

N R Hackett, P D Bragg
PMCID: PMC217520  PMID: 6341359

Abstract

Redox titration has been coupled to spectroscopic techniques, enzyme fractionation, and the use of mutants to examine the cytochrome composition of the membranes from cells grown aerobically and anaerobically with nitrate. A combination of techniques was found to be necessary to resolve the cytochromes. At least six b-type cytochromes were present. Besides cytochromes bfdh and bnr, components of the formate dehydrogenase-nitrate reductase pathway, cytochromes b556, b555, b562, and o, characteristic of aerobic respiratory pathways, were present. The midpoint oxidation-reduction potentials of the aerobic b-type cytochromes suggested that the sequence of electron transfer is: cytochrome b556 leads to b555 leads to b562 leads to O2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DEEB S. S., HAGER L. P. CRYSTALLINE CYTOCHROME B1 FROM ESCHERICHIA COLI. J Biol Chem. 1964 Apr;239:1024–1031. [PubMed] [Google Scholar]
  3. Daniel R. M. The electron transport system of Acetobacter suboxydans with particular reference to cytochrome. Biochim Biophys Acta. 1970 Sep 1;216(2):328–341. doi: 10.1016/0005-2728(70)90224-0. [DOI] [PubMed] [Google Scholar]
  4. Dutton P. L. Redox potentiometry: determination of midpoint potentials of oxidation-reduction components of biological electron-transfer systems. Methods Enzymol. 1978;54:411–435. doi: 10.1016/s0076-6879(78)54026-3. [DOI] [PubMed] [Google Scholar]
  5. Enoch H. G., Lester R. L. The purification and properties of formate dehydrogenase and nitrate reductase from Escherichia coli. J Biol Chem. 1975 Sep 10;250(17):6693–6705. [PubMed] [Google Scholar]
  6. Enoch H. G., Lester R. L. The role of a novel cytochrome b-containing nitrate reductase and quinone in the in vitro reconstruction of formate-nitrate reductase activity of E. coli. Biochem Biophys Res Commun. 1974 Dec 23;61(4):1234–1241. doi: 10.1016/s0006-291x(74)80416-x. [DOI] [PubMed] [Google Scholar]
  7. Hackett N. R., Bragg P. D. Membrane cytochromes of Escherichia coli chl mutants. J Bacteriol. 1983 May;154(2):719–727. doi: 10.1128/jb.154.2.719-727.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Haddock B. A., Downie J. A., Garland P. B. Kinetic characterization of the membrane-bound cytochromes of Escherichia coli grown under a variety of conditions by using a stopped-flow dual-wavelength spectrophotometer. Biochem J. 1976 Feb 15;154(2):285–294. doi: 10.1042/bj1540285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haddock B. A., Jones C. W. Bacterial respiration. Bacteriol Rev. 1977 Mar;41(1):47–99. doi: 10.1128/br.41.1.47-99.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hendler R. W., Shrager R. I. Potentiometric analysis of Escherichia coli cytochromes in the optical absorbance range of 500 nm to 700 nm. J Biol Chem. 1979 Nov 25;254(22):11288–11299. [PubMed] [Google Scholar]
  11. Kita K., Kasahara M., Anraku Y. Formation of a membrane potential by reconstructed liposomes made with cytochrome b562-o complex, a terminal oxidase of Escherichia coli K12. J Biol Chem. 1982 Jul 25;257(14):7933–7935. [PubMed] [Google Scholar]
  12. Kita K., Yamato I., Anraku Y. Purification and properties of cytochrome b556 in the respiratory chain of aerobically grown Escherichia coli K12. J Biol Chem. 1978 Dec 25;253(24):8910–8915. [PubMed] [Google Scholar]
  13. Mitchell P. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc. 1966 Aug;41(3):445–502. doi: 10.1111/j.1469-185x.1966.tb01501.x. [DOI] [PubMed] [Google Scholar]
  14. Pudek M. R., Bragg P. D. Redox potentials of the cytochromes in the respiratory chain of aerobically grown Escherichia coli. Arch Biochem Biophys. 1976 Jun;174(2):546–552. doi: 10.1016/0003-9861(76)90382-9. [DOI] [PubMed] [Google Scholar]
  15. Reid G. A., Ingledew W. J. Characterization and phenotypic control of the cytochrome content of Escherichia coli. Biochem J. 1979 Aug 15;182(2):465–472. doi: 10.1042/bj1820465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reid G. A., Ingledew W. J. The purification of a respiratory oxidase complex from Escherichia coli. FEBS Lett. 1980 Jan 1;109(1):1–4. doi: 10.1016/0014-5793(80)81297-x. [DOI] [PubMed] [Google Scholar]
  17. Ruiz-Herrera J., DeMoss J. A. Nitrate reductase complex of Escherichia coli K-12: participation of specific formate dehydrogenase and cytochrome b1 components in nitrate reduction. J Bacteriol. 1969 Sep;99(3):720–729. doi: 10.1128/jb.99.3.720-729.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scott R. I., Poole R. K. A re-examination of the cytochromes of Escherichia coli using fourth-order finite difference analysis: their characterization under different growth conditions and accumulation during the cell cycle. J Gen Microbiol. 1982 Aug;128(8):1685–1696. doi: 10.1099/00221287-128-8-1685. [DOI] [PubMed] [Google Scholar]
  19. Shipp W. S. Cytochromes of Escherichia coli. Arch Biochem Biophys. 1972 Jun;150(2):459–472. doi: 10.1016/0003-9861(72)90063-x. [DOI] [PubMed] [Google Scholar]
  20. Sánchez Crispín J. A., Dubourdieu M., Chippaux M. Localization and characterization of cytochromes from membrane vesicles of Escherichia coli K-12 grown in anaerobiosis with nitrate. Biochim Biophys Acta. 1979 Aug 14;547(2):198–210. doi: 10.1016/0005-2728(79)90003-3. [DOI] [PubMed] [Google Scholar]
  21. Van Wielink J. E., Oltmann L. F., Leeuwerik F. J., De Hollander J. A., Stouthamer A. H. A method for in situ characterization of b- and c-type cytochromes in Escherichia coli and in complex III from beef heart mitochondria by combined spectrum deconvolution and potentiometric analysis. Biochim Biophys Acta. 1982 Aug 20;681(2):177–190. doi: 10.1016/0005-2728(82)90021-4. [DOI] [PubMed] [Google Scholar]

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