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. 1983 May;154(2):870–878. doi: 10.1128/jb.154.2.870-878.1983

Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane.

M J de Smet, G Eggink, B Witholt, J Kingma, H Wynberg
PMCID: PMC217541  PMID: 6841319

Abstract

The growth of Pseudomonas oleovorans on n-octane was characterized by the formation of intracellular structures. These inclusions were isolated and characterized. Morphologically, they resembled the poly-beta-hydroxybutyrate granules found in Bacillus cereus, as shown by freeze-fracture electron microscopy. The elemental analysis of isolated granules showed, however, that they do not contain poly-beta-hydroxybutyric acid. Instead, the analysis was consistent with a C8 polyester, which interpretation was supported by the fatty acid analysis of hydrolyzed granules. From the evidence presented here, we conclude that P. oleovorans forms poly-beta-hydroxyoctanoate granules when grown on n-octane.

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Selected References

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  1. Dunlop W. F., Robards A. W. Ultrastructural study of poly- -hydroxybutyrate granules from Bacillus cereus. J Bacteriol. 1973 Jun;114(3):1271–1280. doi: 10.1128/jb.114.3.1271-1280.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. LAW J. H., SLEPECKY R. A. Assay of poly-beta-hydroxybutyric acid. J Bacteriol. 1961 Jul;82:33–36. doi: 10.1128/jb.82.1.33-36.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Matin A., Veldhuis C., Stegeman V., Veenhuis M. Selective advantage of a Spirillum sp. in a carbon-limited environment. Accumulation of poly-beta-hydroxybutyric acid and its role in starvation. J Gen Microbiol. 1979 Jun;112(2):349–355. doi: 10.1099/00221287-112-2-349. [DOI] [PubMed] [Google Scholar]
  4. Osborn M. J., Gander J. E., Parisi E., Carson J. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972 Jun 25;247(12):3962–3972. [PubMed] [Google Scholar]
  5. Schwartz R. D. Octene epoxidation by a cold-stable alkane-oxidizing isolate of Pseudomonas oleovorans. Appl Microbiol. 1973 Apr;25(4):574–577. doi: 10.1128/am.25.4.574-577.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Scott C. C., Finnerty W. R. A comparative analysis of the ultrastructure of hydrocarbon-oxidizing micro-organisms. J Gen Microbiol. 1976 Jun;94(2):342–350. doi: 10.1099/00221287-94-2-342. [DOI] [PubMed] [Google Scholar]
  7. Scott C. C., Finnerty W. R. Characterization of intracytoplasmic hydrocarbon inclusions from the hydrocarbon-oxidizing Acinetobacter species HO1-N. J Bacteriol. 1976 Jul;127(1):481–489. doi: 10.1128/jb.127.1.481-489.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Shively J. M. Inclusion bodies of prokaryotes. Annu Rev Microbiol. 1974;28(0):167–187. doi: 10.1146/annurev.mi.28.100174.001123. [DOI] [PubMed] [Google Scholar]
  9. Sleytr U. B., Robards A. W. Plastic deformation during freeze-cleavage: a review. J Microsc. 1977 May;110(1):1–25. doi: 10.1111/j.1365-2818.1977.tb00009.x. [DOI] [PubMed] [Google Scholar]
  10. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  11. Witholt B., Boekhout M., Brock M., Kingma J., Heerikhuizen H. V., Leij L. D. An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coli. Anal Biochem. 1976 Jul;74(1):160–170. doi: 10.1016/0003-2697(76)90320-1. [DOI] [PubMed] [Google Scholar]
  12. Witholt B. Method for isolating mutants overproducing nicotinamide adenine dinucleotide and its precursors. J Bacteriol. 1972 Jan;109(1):350–364. doi: 10.1128/jb.109.1.350-364.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Yan L. P., Hitchins A. D. Comparative macromolecular composition of filaments and rods of a Bacillus megaterium thermoconditional morphological mutant. J Bacteriol. 1980 Oct;144(1):454–456. doi: 10.1128/jb.144.1.454-456.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. de Smet M. J., Wynberg H., Witholt B. Synthesis of 1,2-Epoxyoctane by Pseudomonas oleovorans During Growth in a Two-Phase System Containing High Concentrations of 1-Octene. Appl Environ Microbiol. 1981 Nov;42(5):811–816. doi: 10.1128/aem.42.5.811-816.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. van Heerikhuizen H., Kwak E., van Bruggen E. F., Witholt B. Characterization of a low density cytoplasmic membrane subfraction isolated from Escherichia coli. Biochim Biophys Acta. 1975 Dec 1;413(2):177–191. doi: 10.1016/0005-2736(75)90102-9. [DOI] [PubMed] [Google Scholar]

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