Abstract
Cyanophycin granule polypeptide content increased by 2- to 3-fold, soluble protein content decreased by 1.5-fold, and carbohydrate content increased by 2-fold within 6 h of chloramphenicol addition to exponentially growing cells of Aphanocapsa sp. strain 6308. Analysis of 14C- and 3H-labeled cells transferred to unlabeled medium and analysis of pulse-labeled cells both suggested cyanophycin granule synthesis from preformed protein breakdown.
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Selected References
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- Allen M. M., Hutchison F., Weathers P. J. Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308. J Bacteriol. 1980 Feb;141(2):687–693. doi: 10.1128/jb.141.2.687-693.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Allen M. M., Weathers P. J. Structure and composition of cyanophycin granules in the cyanobacterium Aphanocapsa 6308. J Bacteriol. 1980 Feb;141(2):959–962. doi: 10.1128/jb.141.2.959-962.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hoare D. S., Hoare S. L. Feedback regulation of arginine biosynthesis in blue-green algae and photosynthetic bacteria. J Bacteriol. 1966 Aug;92(2):375–379. doi: 10.1128/jb.92.2.375-379.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hood W., Carr N. G. Apparent lack of control by repression of arginine metabolism in blue-green algae. J Bacteriol. 1971 Jul;107(1):365–367. doi: 10.1128/jb.107.1.365-367.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simon R. D. Measurement of the cyanophycin granule polypeptide contained in the blue-green alga Anabaena cylindrica. J Bacteriol. 1973 Jun;114(3):1213–1216. doi: 10.1128/jb.114.3.1213-1216.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simon R. D. The effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue green alga Anabaena cylindrica. Arch Mikrobiol. 1973;92(2):115–122. doi: 10.1007/BF00425009. [DOI] [PubMed] [Google Scholar]
- Simon R. D., Weathers P. Determination of the structure of the novel polypeptide containing aspartic acid and arginine which is found in Cyanobacteria. Biochim Biophys Acta. 1976 Jan 20;420(1):165–176. doi: 10.1016/0005-2795(76)90355-x. [DOI] [PubMed] [Google Scholar]
- Spector T. Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein. Anal Biochem. 1978 May;86(1):142–146. doi: 10.1016/0003-2697(78)90327-5. [DOI] [PubMed] [Google Scholar]
- Weathers P. J., Allen M. M. Variations in short term products of inorganic carbon fixation in exponential and stationary phase cultures of Aphanocapsa 6308. Arch Microbiol. 1978 Mar;116(3):231–234. doi: 10.1007/BF00417844. [DOI] [PubMed] [Google Scholar]