Abstract
Heterocyst-free (NH4+-grown) cultures of the cyanobacterium Anabaena variabilis produce a hydrogenase which is reversibly inhibited by light and O2. White or red light at an intensity of 5,000 lx inhibited greater than 95% of the activity. Oxygen at concentrations as low as 0.5% inhibited more than 85% of the hydrogenase in the vegetative cells of CO2-NH4+-grown cultures. The vegatative cell hydrogenase is also sensitive to strong oxidants like ferricyanide. In the presence of strong reductants like S2O4(2-), hydrogenase activity was not inhibited by light. However, hydrogenase activity in the heterocysts was insensitive to both light (greater than 5,000 lx) and O2 (10%). Heterocysts and light-insensitive hydrogenase activity appear simultaneously during differentiation of the vegetative cells into heterocysts (an NH4+-grown culture transferred to NH4+-free, N2-containing medium). This light-insensitive hydrogenase activity was detected several hours before the induction of nitrogenase activity. These results suggest a mode of regulation of hydrogenase in the vegetative cells of A. variabilis that is similar to "redox control" of hydrogenase and other "anaerobic" proteins in enteric bacteria like Escherichia coli.
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- Adams M. W., Mortenson L. E., Chen J. S. Hydrogenase. Biochim Biophys Acta. 1980 Dec;594(2-3):105–176. doi: 10.1016/0304-4173(80)90007-5. [DOI] [PubMed] [Google Scholar]
- Benemann J. R., Weare N. M. Hydrogen Evolution by Nitrogen-Fixing Anabaena cylindrica Cultures. Science. 1974 Apr 12;184(4133):174–175. doi: 10.1126/science.184.4133.174. [DOI] [PubMed] [Google Scholar]
- Burgess B. K., Wherland S., Newton W. E., Stiefel E. I. Nitrogenase reactivity: insight into the nitrogen-fixing process through hydrogen-inhibition and HD-forming reactions. Biochemistry. 1981 Sep 1;20(18):5140–5146. doi: 10.1021/bi00521a007. [DOI] [PubMed] [Google Scholar]
- Haury J. F., Spiller H. Fructose uptake and influence on growth of and nitrogen fixation by Anabaena variabilis. J Bacteriol. 1981 Jul;147(1):227–235. doi: 10.1128/jb.147.1.227-235.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Houchins J. P., Burris R. H. Occurrence and localization of two distinct hydrogenases in the heterocystous cyanobacterium Anabaena sp. strain 7120. J Bacteriol. 1981 Apr;146(1):209–214. doi: 10.1128/jb.146.1.209-214.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lambert G. R., Smith G. D. Hydrogen metabolism by filamentous cyanobacteria. Arch Biochem Biophys. 1980 Nov;205(1):36–50. doi: 10.1016/0003-9861(80)90081-8. [DOI] [PubMed] [Google Scholar]
- Lim S. T. Determination of Hydrogenase in Free-living Cultures of Rhizobium japonicum and Energy Efficiency of Soybean Nodules. Plant Physiol. 1978 Oct;62(4):609–611. doi: 10.1104/pp.62.4.609. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PICHINOTY F. [Inhibition by oxygen of the biosynthesis and activity of hydrogenase and hydrogenlyase in some anaerobic bacteria]. Biochim Biophys Acta. 1962 Oct 8;64:111–124. doi: 10.1016/0006-3002(62)90764-3. [DOI] [PubMed] [Google Scholar]
- Tel-Or E., Luijk L. W., Packer L. An inducible hydrogenase in cyanobacteria enhances n2 fixation. FEBS Lett. 1977;78(1):49–52. doi: 10.1016/0014-5793(77)80270-6. [DOI] [PubMed] [Google Scholar]
- Tel-Or E., Luijk L. W., Packer L. Hydrogenase in N2-fixing cyanobacteria. Arch Biochem Biophys. 1978 Jan 15;185(1):185–194. doi: 10.1016/0003-9861(78)90158-3. [DOI] [PubMed] [Google Scholar]
- Tetley R. M., Bishop N. I. The differential action of metronidazole on nitrogen fixation, hydrogen metabolism, photosynthesis and respiration in Anabaena and Scenedesmus. Biochim Biophys Acta. 1979 Apr 11;546(1):43–53. doi: 10.1016/0005-2728(79)90168-3. [DOI] [PubMed] [Google Scholar]