Abstract
Transport of ferric iron into Corynebacterium diphtheriae C7(beta) was shown to occur by a high-affinity, active transport system. Optimal rates were at pH 6.8 and 40 degrees C. Strong inhibition of uptake by carbonyl cyanide m-chlorophenylhydrazone was consistent with the electrochemical proton gradient as the major energy source for iron transport, and inhibition by Hg2+ indicated that sulfhydryl groups were also important. Evidence was obtained for stimulation of iron uptake at pH 8.0 by a dialyzable, extracellular factor present in conditioned medium from low-iron cultures of C. diphtheriae.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bullen J. J. The significance of iron in infection. Rev Infect Dis. 1981 Nov-Dec;3(6):1127–1138. doi: 10.1093/clinids/3.6.1127. [DOI] [PubMed] [Google Scholar]
- Cryz S. J., Jr, Russell L. M., Holmes R. K. Regulation of toxinogenesis in Corynebacterium diphtheriae: mutations in the bacterial genome that alter the effects of iron on toxin production. J Bacteriol. 1983 Apr;154(1):245–252. doi: 10.1128/jb.154.1.245-252.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friedberg I. The effect of ionophores on phosphate and arsenate transport in Micrococcus lysodeikticus. FEBS Lett. 1977 Sep 15;81(2):264–266. doi: 10.1016/0014-5793(77)80531-0. [DOI] [PubMed] [Google Scholar]
- Groman N., Judge K. Effect of metal ions on diphtheria toxin production. Infect Immun. 1979 Dec;26(3):1065–1070. doi: 10.1128/iai.26.3.1065-1070.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Konings W. N., Rosenberg H. Phosphate transport in membrane vesicles from Escherichia coli. Biochim Biophys Acta. 1978 Apr 4;508(2):370–378. doi: 10.1016/0005-2736(78)90339-5. [DOI] [PubMed] [Google Scholar]
- Mueller J. H. NUTRITION OF THE DIPHTHERIA BACILLUS. Bacteriol Rev. 1940 Jun;4(2):97–134. doi: 10.1128/br.4.2.97-134.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neilands J. B. Microbial iron compounds. Annu Rev Biochem. 1981;50:715–731. doi: 10.1146/annurev.bi.50.070181.003435. [DOI] [PubMed] [Google Scholar]
- Rhoads D. B., Epstein W. Energy coupling to net K+ transport in Escherichia coli K-12. J Biol Chem. 1977 Feb 25;252(4):1394–1401. [PubMed] [Google Scholar]