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. 1983 Nov;156(2):859–866. doi: 10.1128/jb.156.2.859-866.1983

Isolation and characterization of two chondroitin lyases from Bacteroides thetaiotaomicron.

S Linn, T Chan, L Lipeski, A A Salyers
PMCID: PMC217904  PMID: 6630153

Abstract

Two chondroitin lyases were isolated from the colon anaerobe Bacteroides thetaiotaomicron. Both enzymes had similar molecular weights (104,000 and 108,000) and similar isoelectric points (8.0 and 7.9, respectively). Both enzymes were active against chondroitin sulfates A, B, and C and unsulfated polysaccharides, such as chondroitin and hyaluronic acid, although one of the enzymes was twice as active against chondroitin as the other enzyme. Both had similar Km values for chondroitin sulfates A and C (40 to 70 micrograms/ml) and for chondroitin (300 to 400 micrograms/ml). Neither enzyme could degrade the highly sulfated mucopolysaccharide heparin, but heparin was a potent inhibitor of the activity of both enzymes. Although enzymes I and II were similar in many respects, a comparison of peptides resulting from partial digestion with N-chlorosuccinimide or papain demonstrated that the two proteins are not related.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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