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. 1979 Sep;139(3):705–712. doi: 10.1128/jb.139.3.705-712.1979

Transport systems for branched-chain amino acids in Pseudomonas aeruginosa.

T Hoshino
PMCID: PMC218013  PMID: 113383

Abstract

The cells of Pseudomonas aeruginosa showed high activity for leucine transport in the absence of Na+, giving a Km value of 0.34 microM. In the presence of Na+, however, two Km values, 0.37 microM (LIV-I system) and 7.6 microM (LIV-II system), were obtained. The former system seemed to serve not only for the entry of leucine, isoleucine, and valine, but also for that of alanine and threonine, although less effectively. However, the LIV-II system served for the entry of branched-chain amino acids only. The LIV-II system alone was operative in membrane vesicles, for the transport of branched-chain amino acids in membrane vesicles required Na+ and gave single Km values for the respective amino acids. When cells were osmotically shocked, the activity of the LIV-I system decreased, whereas the LIV-II system remained unaffected. The shock fluid from P. aeruginosa cells showed leucine-binding activity with a dissociation constant of 0.25 microM. The specificity of the activity was very similar to that of the LIV-I system. These results suggest that a leucine-binding protein(s) in the periplasmic space may be required for the transport process via the LIV-I system of P. aeruginosa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhatti A. R., DeVoe I. W., Ingram J. M. The release and characterization of some periplasm-located enzymes of Pseudomona aeruginosa. Can J Microbiol. 1976 Oct;22(10):1425–1429. doi: 10.1139/m76-211. [DOI] [PubMed] [Google Scholar]
  2. Cheng K. J., Ingram J. M., Costerton J. W. Interactions of alkaline phosphatase and the cell wall of Pseudomonas aeruginosa. J Bacteriol. 1971 Jul;107(1):325–336. doi: 10.1128/jb.107.1.325-336.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fein J. E., MacLeod R. A. Characterization of neutral amino acid transport in a marine pseudomonad. J Bacteriol. 1975 Dec;124(3):1177–1190. doi: 10.1128/jb.124.3.1177-1190.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guardiola J., De Felice M., Klopotowski T., Iaccarino M. Multiplicity of isoleucine, leucine, and valine transport systems in Escherichia coli K-12. J Bacteriol. 1974 Feb;117(2):382–392. doi: 10.1128/jb.117.2.382-392.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hoshino T., Kageyama M. Sodium-dependent transport of L-leucine in membrane vesicles prepared from Pseudomonas aeruginosa. J Bacteriol. 1979 Jan;137(1):73–81. doi: 10.1128/jb.137.1.73-81.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kay W. W., Gronlund A. F. Influence of carbon or nitrogen starvation on amino acid transport in Pseudomonas aeruginosa. J Bacteriol. 1969 Oct;100(1):276–282. doi: 10.1128/jb.100.1.276-282.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kiritani K., Ohnishi K. Repression and inhibition of transport systems for branched-chain amino acids in Salmonella typhimurium. J Bacteriol. 1977 Feb;129(2):589–598. doi: 10.1128/jb.129.2.589-598.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kobayashi H., Kin E., Anraku Y. Transport of sugars and amino acids in bacteria. X. Sources of energy and energy coupling reactions of the active transport systems for isoleucine and proline in E. coli. J Biochem. 1974 Aug;76(2):251–261. doi: 10.1093/oxfordjournals.jbchem.a130567. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Neal J. L. Analysis of Michaelis kinetics for two independent, saturable membrane transport functions. J Theor Biol. 1972 Apr;35(1):113–118. doi: 10.1016/0022-5193(72)90196-8. [DOI] [PubMed] [Google Scholar]
  11. Oxender D. L. Membrane transport. Annu Rev Biochem. 1972;41(10):777–814. doi: 10.1146/annurev.bi.41.070172.004021. [DOI] [PubMed] [Google Scholar]
  12. Pearce S. M., Hildebrandt V. A., Lee T. Third system for neutral amino acid transport in a marine pseudomonad. J Bacteriol. 1977 Apr;130(1):37–47. doi: 10.1128/jb.130.1.37-47.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rahmanian M., Claus D. R., Oxender D. L. Multiplicity of leucine transport systems in Escherichia coli K-12. J Bacteriol. 1973 Dec;116(3):1258–1266. doi: 10.1128/jb.116.3.1258-1266.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Stinson M. W., Cohen M. A., Merrick J. M. Isolation of dicarboxylic acid- and glucose-binding proteins from Pseudomonas aeruginosa. J Bacteriol. 1976 Nov;128(2):573–579. doi: 10.1128/jb.128.2.573-579.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stinson M. W., Cohen M. A., Merrick J. M. Purification and properties of the periplasmic glucose-binding protein of Pseudomonas aeruginosa. J Bacteriol. 1977 Aug;131(2):672–681. doi: 10.1128/jb.131.2.672-681.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Tsay S. S., Brown K. K., Gaudy E. T. Transport of glycerol by Pseudomonas aeruginosa. J Bacteriol. 1971 Oct;108(1):82–88. doi: 10.1128/jb.108.1.82-88.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wood J. M. Leucine transport in Escherichia coli. The resolution of multiple transport systems and their coupling to metabolic energy. J Biol Chem. 1975 Jun 25;250(12):4477–4485. [PubMed] [Google Scholar]
  18. Yamato I., Anraku Y. Transport of sugars and amino acids in bacteria. XVIII. Properties of an isoleucine carrier in the cytoplasmic membrane vesicles of Escherichia coli. J Biochem. 1977 May;81(5):1517–1523. [PubMed] [Google Scholar]

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