Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Sep;139(3):917–923. doi: 10.1128/jb.139.3.917-923.1979

Oxalurate transport in Saccharomyces cerevisiae.

T G Cooper, J McKelvey, R Sumrada
PMCID: PMC218039  PMID: 383700

Abstract

Oxalurate, the gratuitous inducer of the allantoin degradative enzymes, was taken into the cell by an energy-dependent active transport system with an apparent Km of 1.2 mM. Efflux of previously accumulated oxalurate was rapid, with a half-life of about 2 min. The oxalurate uptake system appears to be both constitutively produced and insensitive to nitrogen catabolite repression. The latter observations suggest that failure of oxalurate to bring about induction of allophanate hydrolase in cultures growing under repressive conditions does not result from inducer exclusion, but rather from repression of dur1,2 gene expression.

Full text

PDF
923

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bossinger J., Cooper T. G. Execution times of macromolecular synthetic processes involved in the induction of allophanate hydrolase at 15 degrees C. J Bacteriol. 1976 Oct;128(1):498–501. doi: 10.1128/jb.128.1.498-501.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bossinger J., Cooper T. G. Sequence of molecular events involved in induction of allophanate hydrolase. J Bacteriol. 1976 Apr;126(1):198–204. doi: 10.1128/jb.126.1.198-204.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bossinger J., Cooper T. Possible failure of NADP-glutamate dehydrogenase to participate directly in nitrogen repression of the allantoin degradative enzymes in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1975 Oct 6;66(3):889–892. doi: 10.1016/0006-291x(75)90723-8. [DOI] [PubMed] [Google Scholar]
  4. Bossinger J., Lawther R. P., Cooper T. G. Nitrogen repression of the allantoin degradative enzymes in Saccharomyces cerevisiae. J Bacteriol. 1974 Jun;118(3):821–829. doi: 10.1128/jb.118.3.821-829.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooper T. G., Beevers H. Mitochondria and glyoxysomes from castor bean endosperm. Enzyme constitutents and catalytic capacity. J Biol Chem. 1969 Jul 10;244(13):3507–3513. [PubMed] [Google Scholar]
  6. Cooper T. G., Sumrada R. Urea transport in Saccharomyces cerevisiae. J Bacteriol. 1975 Feb;121(2):571–576. doi: 10.1128/jb.121.2.571-576.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lawther R. P., Cooper T. G. Effects of inducer addition and removal upon the level of allophanate hydrolase in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1973 Dec 19;55(4):1100–1104. doi: 10.1016/s0006-291x(73)80008-7. [DOI] [PubMed] [Google Scholar]
  8. Lawther R. P., Cooper T. G. Kinetics of induced and repressed enzyme synthesis in Saccharomyces cerevisiae. J Bacteriol. 1975 Mar;121(3):1064–1073. doi: 10.1128/jb.121.3.1064-1073.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lawther R. P., Riemer E., Chojnacki B., Cooper T. G. Clustering of the genes for allantoin degradation in Saccharomyces cerevisiae. J Bacteriol. 1974 Aug;119(2):461–468. doi: 10.1128/jb.119.2.461-468.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sumrada R., Cooper T. G. Allantoin transport in Saccharomyces cerevisiae. J Bacteriol. 1977 Sep;131(3):839–847. doi: 10.1128/jb.131.3.839-847.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sumrada R., Cooper T. G. Oxaluric acid: a non-metabolizable inducer of the allantoin degradative enzymes in Saccharomyces cerevisiae. J Bacteriol. 1974 Mar;117(3):1240–1247. doi: 10.1128/jb.117.3.1240-1247.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sumrada R., Zacharski C. A., Turoscy V., Cooper T. G. Induction and inhibition of the allantoin permease in Saccharomyces cerevisiae. J Bacteriol. 1978 Aug;135(2):498–510. doi: 10.1128/jb.135.2.498-510.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Whitney P. A., Cooper T. G., Magasanik B. The induction of urea carboxylase and allophanate hydrolase in Saccharomyces cerevisiae. J Biol Chem. 1973 Sep 10;248(17):6203–6209. [PubMed] [Google Scholar]
  14. Whitney P. A., Cooper T. G. Requirement for HCO3- by ATP: urea amido-lyase in yeast. Biochem Biophys Res Commun. 1970 Aug 24;40(4):814–819. doi: 10.1016/0006-291x(70)90975-7. [DOI] [PubMed] [Google Scholar]
  15. Whitney P. A., Cooper T. G. Urea carboxylase and allophanate hydrolase. Two components of adenosine triphosphate:urea amido-lyase in Saccharomyces cerevisiae. J Biol Chem. 1972 Mar 10;247(5):1349–1353. [PubMed] [Google Scholar]
  16. Wickerham L. J. A Critical Evaluation of the Nitrogen Assimilation Tests Commonly Used in the Classification of Yeasts. J Bacteriol. 1946 Sep;52(3):293–301. [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES