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. 1979 Jun;138(3):748–755. doi: 10.1128/jb.138.3.748-755.1979

Suppressor mutation in Pseudomonas aeruginosa.

M Kageyama, Y Sano, T Shinomiya
PMCID: PMC218100  PMID: 110767

Abstract

Suppressor mutations were identified in Pseudomonas aeruginosa, and a comparison was made with Escherichia coli suppressor systems. A suppressor-sensitive (sus) derivative of a plasmid, RP4 trp, and several Sus mutants of IncP1 plasmid-specific phages, were isolated by using E. coli. Plasmid RP4 trp (sus) was transferred to P. aeruginosa strains carrying trp markers which did not complement RP4 trp(sus), and Trp+ variants were selected. Some, but not all such revertants, could propagate PRD1 Sus phages, and these mutants were found to be supressor positive. Plating efficiencies of various Sus phages on these strains were compared with on E. coli strains carrying known suppressor genes. The results suggested that the Pseudomonas suppressors were probably amber suppressors. In iddition, some Sus phages (PRD1sus-55, PRD1sus-56) were obtained which, although apparently of the amber type for E. coli, were able to propagate equally well on sup+ or sup strains of P. aeruginosa. On the other hand, several mutants of phage PRR1 which were suppressed in E. coli were not suppressed by the P. aeruginosa suppressor. Suppressor-sensitive mutants were also isolated with P. aeruginosa bacteriophages E79 and D3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chandler P. M., Krishnapillai V. Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors readily transferable between Pseudomonas and the Enterobacteriaceae. Genet Res. 1974 Jun;23(3):239–250. doi: 10.1017/s0016672300014890. [DOI] [PubMed] [Google Scholar]
  2. Crawford I. P. Gene rearrangements in the evolution of the tryptophan synthetic pathway. Bacteriol Rev. 1975 Jun;39(2):87–120. doi: 10.1128/br.39.2.87-120.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. EGAN J. B., HOLLOWAY B. W. Genetic studies on lysogeny in Pseudomonas aeruginosa. Aust J Exp Biol Med Sci. 1961 Feb;39:9–17. doi: 10.1038/icb.1961.2. [DOI] [PubMed] [Google Scholar]
  4. Jacoby G. A. Properties of R plasmids determining gentamicin resistance by acetylation in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1974 Sep;6(3):239–252. doi: 10.1128/aac.6.3.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Krishnapillai V. A novel transducing phage. Its role in recognition of a possible new host-controlled modification system in Pseudomonas aeruginosa. Mol Gen Genet. 1972;114(2):134–143. doi: 10.1007/BF00332784. [DOI] [PubMed] [Google Scholar]
  6. Miller R. V., Pemberton J. M., Richards K. E. F116, D3 and G101: temperate bacteriophages of Pseudomonas aeruginosa. Virology. 1974 Jun;59(2):566–569. doi: 10.1016/0042-6822(74)90466-8. [DOI] [PubMed] [Google Scholar]
  7. Mindich L., Cohen J., Weisburd M. Isolation of nonsense suppressor mutants in Pseudomonas. J Bacteriol. 1976 Apr;126(1):177–182. doi: 10.1128/jb.126.1.177-182.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nagahari K., Sano Y., Sakaguchi K. Derepression of E. coli trp operon on interfamilial transfer. Nature. 1977 Apr 21;266(5604):745–746. doi: 10.1038/266745a0. [DOI] [PubMed] [Google Scholar]
  9. Nagahari K., Tanaka T., Hishinuma F., Kuroda M., Sakaguchi K. Control of tryptophan synthetase amplified by varying the numbers of composite plasmids in Escherichia coli cells. Gene. 1977 Mar;1(2):141–152. doi: 10.1016/0378-1119(77)90025-7. [DOI] [PubMed] [Google Scholar]
  10. Nomura Y. Irreversible inactivation at high temperature of temperature-sensitive mutant tRNA Tyr in vivo. Nat New Biol. 1973 Mar 7;242(114):12–14. doi: 10.1038/newbio242012a0. [DOI] [PubMed] [Google Scholar]
  11. Olsen R. H., Siak J. S., Gray R. H. Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage. J Virol. 1974 Sep;14(3):689–699. doi: 10.1128/jvi.14.3.689-699.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Olsen R. H., Thomas D. D. Characteristics and purification of PRR1, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid. J Virol. 1973 Dec;12(6):1560–1567. doi: 10.1128/jvi.12.6.1560-1567.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SLAYTER H. S., HOLLOWAY B. W., HALL C. E. THE STRUCTURE OF PSEUDOMONAS AERUGINOSA PHAGES B3, E79, AND F116. J Ultrastruct Res. 1964 Oct;11:274–281. doi: 10.1016/s0022-5320(64)90032-2. [DOI] [PubMed] [Google Scholar]
  14. Tessman I. Mutagenic treatment of double- and single-stranded DNA phages T4 ans S13 with hydroxylamine. Virology. 1968 Jun;35(2):330–333. doi: 10.1016/0042-6822(68)90275-4. [DOI] [PubMed] [Google Scholar]
  15. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]
  16. Waltho J. A., Holloway B. W. Suppression of fluorophenylalanine resistance by mutation to streptomycin resistance in Pseudomonas aeruginosa. J Bacteriol. 1966 Jul;92(1):35–42. doi: 10.1128/jb.92.1.35-42.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Watson J. M., Holloway B. W. Suppressor mutations in Pseudomonas aeruginosa. J Bacteriol. 1976 Mar;125(3):780–786. doi: 10.1128/jb.125.3.780-786.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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