Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 Jun;138(3):832–838. doi: 10.1128/jb.138.3.832-838.1979

Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli.

M Kitakawa, E R Dabbs, K Isono
PMCID: PMC218111  PMID: 378941

Abstract

Mutants with alterations in the structural genes for ribosomal proteins S15, L21, and L27 were used in mapping the genes coding for these proteins. Results from P1kc-mediated transductions indicate that the genes for L21 (rplU) and L27 (rpmA) form a gene cluster and are located between argG and gltB at 68.1 min, whereas the gene for S15 (rpsO) is situated close to, but on the opposite side or, argG. The gene order in this region is concluded to be gltB-(rplU, rpmA)-argG-rpsO-mtr.

Full text

PDF
833

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beckwith J. R., Signer E. R., Epstein W. Transposition of the Lac region of E. coli. Cold Spring Harb Symp Quant Biol. 1966;31:393–401. doi: 10.1101/sqb.1966.031.01.051. [DOI] [PubMed] [Google Scholar]
  3. Berberich M. A. A glutamate-dependent phenotype in E. coli K12: the result of two mutations. Biochem Biophys Res Commun. 1972 Jun 28;47(6):1498–1503. doi: 10.1016/0006-291x(72)90242-2. [DOI] [PubMed] [Google Scholar]
  4. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dabbs E. R. Mutational alterations in 50 proteins of the Escherichia coli ribosome. Mol Gen Genet. 1978 Sep 20;165(1):73–78. doi: 10.1007/BF00270378. [DOI] [PubMed] [Google Scholar]
  6. Dabbs E. R., Wittman H. G. A strain of Escherichia coli which gives rise to mutations in a large number of ribosomal proteins. Mol Gen Genet. 1976 Dec 22;149(3):303–309. doi: 10.1007/BF00268532. [DOI] [PubMed] [Google Scholar]
  7. Gesteland R. F. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J Mol Biol. 1966 Mar;16(1):67–84. doi: 10.1016/s0022-2836(66)80263-2. [DOI] [PubMed] [Google Scholar]
  8. Isono K., Cumberlidge A. G., Isono S. Further temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins. Mol Gen Genet. 1977 Apr 29;152(3):239–243. doi: 10.1007/BF00693076. [DOI] [PubMed] [Google Scholar]
  9. Isono K. Genes encoding ribosomal proteins S16 and L19 form a gene cluster at 56.4 min in Escherichia coli. Mol Gen Genet. 1978 Oct 24;165(3):265–268. doi: 10.1007/BF00332525. [DOI] [PubMed] [Google Scholar]
  10. Isono K., Kitakawa M. A new ribosomal protein locus in Escherichia coli: the gene for protein S6 maps at 97 min. Mol Gen Genet. 1977 Jun 8;153(2):115–120. doi: 10.1007/BF00264725. [DOI] [PubMed] [Google Scholar]
  11. Isono K., Kitakawa M. Cluster of ribosomal protein genes in Escherichia coli containing genes for proteins S6, S18, and L9. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6163–6167. doi: 10.1073/pnas.75.12.6163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Isono K., Krauss J., Hirota Y. Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins. Mol Gen Genet. 1976 Dec 22;149(3):297–302. doi: 10.1007/BF00268531. [DOI] [PubMed] [Google Scholar]
  13. Isono S., Isono K. Mutations affecting the structural genes and the genes coding for modifying enzymes for ribosomal proteins in Escherichia coli. Mol Gen Genet. 1978 Sep 20;165(1):15–20. doi: 10.1007/BF00270371. [DOI] [PubMed] [Google Scholar]
  14. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. doi: 10.1016/0003-2697(70)90376-3. [DOI] [PubMed] [Google Scholar]
  15. Nomura M., Morgan E. A. Genetics of bacterial ribosomes. Annu Rev Genet. 1977;11:297–347. doi: 10.1146/annurev.ge.11.120177.001501. [DOI] [PubMed] [Google Scholar]
  16. STACEY K. A., SIMSON E. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:554–555. doi: 10.1128/jb.90.2.554-555.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Takata R. Genetic studies of the ribosomal proteins in Escherichia coli. XI. Mapping of the genes for L21, L27, S15 and S21 by using hybrid bacteria and over-production of these proteins in the merodiploid strains. Mol Gen Genet. 1978 Apr 6;160(2):151–155. doi: 10.1007/BF00267476. [DOI] [PubMed] [Google Scholar]
  18. Takata R., Kobata K. Genetic studies of the ribosomal proteins in Escherichia coli. X. Mapping of the ribosomal proteins, L21 and S15, by intergeneric mating experiments between Serratia marcescens and Escherichia coli K12. Mol Gen Genet. 1976 Dec 8;149(2):159–165. doi: 10.1007/BF00332884. [DOI] [PubMed] [Google Scholar]
  19. Wu T. T. A model for three-point analysis of random general transduction. Genetics. 1966 Aug;54(2):405–410. doi: 10.1093/genetics/54.2.405. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES