Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1979 May;138(2):559–566. doi: 10.1128/jb.138.2.559-566.1979

Mutants of the mini-F plasmid pML31 thermosensitive in replication.

R Eichenlaub
PMCID: PMC218213  PMID: 374396

Abstract

Hydroxylamine mutagenesis was used for the induction of thermosensitive replication mutants of the mini-F plasmid pML31. Replication mutants were characterized by studying the segregation kinetics and the incorporation of [3H]-thymidine into plasmid deoxyribonucleic acid at the nonpermissive temperature. Based on these experiments two types of mutants could be distinguished. Mutants of type I are fast segregating with the kinetics expected if plasmid replication was blocked immediately. Double-label experiments showed a rapid shut-off of replication in these mutants at 42 degrees C. Mutants of type II segregate slower, showing only a partial inhibition of plasmid deoxyribonucleic acid synthesis at the nonpermissive temperature. The label incorporated at 42 degrees C was predominantly found in open circular plasmid molecules.

Full text

PDF
562

Images in this article

564Image
on p.564

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cabello F., Timmis K., Cohen S. N. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature. 1976 Jan 29;259(5541):285–290. doi: 10.1038/259285a0. [DOI] [PubMed] [Google Scholar]
  2. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collins J., Yanofsky S., Helinski D. R. Altered DNA-protein relaxation complex in a replication mutant of plasmid ColE1. Mol Gen Genet. 1978 Nov 16;167(1):21–28. doi: 10.1007/BF00270317. [DOI] [PubMed] [Google Scholar]
  4. Cress D. E., Kline B. C. Isolation and characterization of Escherichia coli chromosomal mutants affecting plasmid copy number. J Bacteriol. 1976 Feb;125(2):635–642. doi: 10.1128/jb.125.2.635-642.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cuzin F., Jacob F. Mutations de l'épisome F d'Escherichia coli K 12. II. Mutants à réplication thermosensible. Ann Inst Pasteur (Paris) 1967 Apr;112(4):397–418. [PubMed] [Google Scholar]
  6. DeVries J. K., Maas W. K. Description of an incompatibility mutant of Escherichia coli. J Bacteriol. 1973 Jul;115(1):213–220. doi: 10.1128/jb.115.1.213-220.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dubnau E., Maas W. K. Inhibition of replication of an F'lac episome in Hfr cells of Escherichia coli. J Bacteriol. 1968 Feb;95(2):531–539. doi: 10.1128/jb.95.2.531-539.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Eichenlaub R., Figurski D., Helinski D. R. Bidirection replication from a unique origin in a mini-F plasmid. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1138–1141. doi: 10.1073/pnas.74.3.1138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goebel W., Schrempf H. Replication of plasmid DNA in temperature-sensitivity DNA replication mutants of Escherichia coli. Biochim Biophys Acta. 1972 Feb 23;262(1):32–41. doi: 10.1016/0005-2787(72)90216-x. [DOI] [PubMed] [Google Scholar]
  10. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gustafsson P., Nordström K. Temperature-dependent and amber copy mutants of plasmid R1drd-19 in Escherichia coli. Plasmid. 1978 Feb;1(2):134–144. doi: 10.1016/0147-619x(78)90034-3. [DOI] [PubMed] [Google Scholar]
  12. Guyer M. S., Figurski D., Davidson N. Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid. J Bacteriol. 1976 Aug;127(2):988–997. doi: 10.1128/jb.127.2.988-997.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hashimoto-Gotoh T., Sekiguchi M. Mutations of temperature sensitivity in R plasmid pSC101. J Bacteriol. 1977 Aug;131(2):405–412. doi: 10.1128/jb.131.2.405-412.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hiraga S., Saito T. Initiation of DNA replication in Escherichia coli. II. Effect of rifampicin on the resumption of replication of F episome and chromosome upon the returning of dna mutants from a non-permissive to a permissive temperature. Mol Gen Genet. 1975;137(3):239–248. doi: 10.1007/BF00333019. [DOI] [PubMed] [Google Scholar]
  15. Humphreys G. O., Willshaw G. A., Smith H. R., Anderson E. S. Mutagenesis of plasmid DNA with hydroxylamine: isolation of mutants of multi-copy plasmids. Mol Gen Genet. 1976 Apr 23;145(1):101–108. doi: 10.1007/BF00331564. [DOI] [PubMed] [Google Scholar]
  16. Jamieson A. F., Bergquist P. L. Role of dnaB43 in F'-plasmid incompatibility. Mol Gen Genet. 1977 Jan 18;150(2):161–170. doi: 10.1007/BF00695396. [DOI] [PubMed] [Google Scholar]
  17. Katz L., Kingsbury D. T., Helinski D. R. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. J Bacteriol. 1973 May;114(2):577–591. doi: 10.1128/jb.114.2.577-591.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lovett M. A., Helinski D. R. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid. J Bacteriol. 1976 Aug;127(2):982–987. doi: 10.1128/jb.127.2.982-987.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nishimura Y., Caro L., Berg C. M., Hirota Y. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome. J Mol Biol. 1971 Feb 14;55(3):441–456. doi: 10.1016/0022-2836(71)90328-7. [DOI] [PubMed] [Google Scholar]
  20. Thompson R., Broda P. DNA polymerase 3 and the replication of F and ColVBtrp in Escherichia coli K-12. Mol Gen Genet. 1973 Dec 31;127(3):255–258. doi: 10.1007/BF00333765. [DOI] [PubMed] [Google Scholar]
  21. Timmis K., Cabello F., Cohen S. N. Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242–2246. doi: 10.1073/pnas.72.6.2242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Van Brunt J., Waggoner B. T., Pato M. L. Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli. Mol Gen Genet. 1977 Feb 15;150(3):285–292. doi: 10.1007/BF00268127. [DOI] [PubMed] [Google Scholar]
  23. Yamamoto T., Kaji A. Replication of thermosensitive Rts1 plasmid deoxyribonucleic acid at the nonpermissive temperature. J Bacteriol. 1977 Oct;132(1):90–99. doi: 10.1128/jb.132.1.90-99.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Zeuthen J., Pato M. L. Replication of the F'lac sex factor in the cell cycle of Escherichia coli. Mol Gen Genet. 1971;111(3):242–255. doi: 10.1007/BF00433109. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES