Abstract
A procedure has been developed that allows the propagation of generalized transducing phage directly on cells growing on solid media. After the donor cells are killed with chloroform, the phage can be transferred directly to recipient cells and transductants can be selected.
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- Boro H., Brenchley J. E. A new generalized transducing phage for Salmonella typhimurium LT2. Virology. 1971 Sep;45(3):835–836. doi: 10.1016/0042-6822(71)90208-x. [DOI] [PubMed] [Google Scholar]
- Brenchley J. E. Effect of methionine sulfoximine and methionine sulfone on glutamate synthesis in Klebsiella aerogenes. J Bacteriol. 1973 May;114(2):666–673. doi: 10.1128/jb.114.2.666-673.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaye R., Barravecchio J., Roth J. Isolation of P22 specialized transducing phage followong F'-episome fusion. Genetics. 1974 Apr;76(4):655–667. doi: 10.1093/genetics/76.4.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kleckner N., Roth J., Botstein D. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. J Mol Biol. 1977 Oct 15;116(1):125–159. doi: 10.1016/0022-2836(77)90123-1. [DOI] [PubMed] [Google Scholar]
- McIntire S. A. Transduction with integration-defective mutants of Salmonella typhimurium bacteriophage KB1. J Bacteriol. 1974 Feb;117(2):907–908. doi: 10.1128/jb.117.2.907-908.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ratzkin B., Roth J. Cluster of genes controlling proline degradation in Salmonella typhimurium. J Bacteriol. 1978 Feb;133(2):744–754. doi: 10.1128/jb.133.2.744-754.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schmieger H. Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet. 1972;119(1):75–88. doi: 10.1007/BF00270447. [DOI] [PubMed] [Google Scholar]
- Steege D. A., Low B. Isolation and characterization of lambda transducing bacteriophages for the su1+ (supD minus) amber suppressor of Escherichia coli. J Bacteriol. 1975 Apr;122(1):120–128. doi: 10.1128/jb.122.1.120-128.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]