Abstract
The carbohydrate portion of fraction I of the Neurospora crassa cell wall has been analyzed for sugar composition by gas-liquid chromatography and colorimetric methods. The analysis was performed comparatively in a wild-type strain (RL 3-8A) and three morphological mutants: scumbo (FGSC 49), peak-2a (a mutant known to be allelic to biscuit), and ragged (FGSC 296). Fraction I of all strains studied contains glucose, mannose, and galactose as the main sugars. Uronic acids and amino sugars are also present in small amounts. The glycosidic linkages binding the neutral sugars were analyzed by Lindberg's combined gas chromatography-mass spectrometry techniques for identification of the partially methylated alditol acitate sugar derivatives. The main polymeric portion of fraction I seems to be a linear glucan with the glucose residues linked by 1 leads to 3 and 1 leads to 4 bonds. A mannan portion with a branched configuration is also present, with galactose as the sugar residue which serves as branches in the molecule(s). The branched mannan portion appears to increase in amount in correlation with more drastic morphological changes of the mycelia. In this respect, the mutant ragged has the lowest mycelial growth rate and the largest amount of mannan. The importance of the polysaccharide structure of fraction I on the colonial morphology of the mycelia is discussed.
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- DE TERRA N., TATUM E. L. Colonial growth of Neurospora. Sorbose and enzymes alter the composition of the cell wall and induce morphological changes. Science. 1961 Oct 13;134(3485):1066–1068. doi: 10.1126/science.134.3485.1066. [DOI] [PubMed] [Google Scholar]
- Elson L. A., Morgan W. T. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. doi: 10.1042/bj0271824. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
- Kocourek J., Ballou C. E. Method for fingerprinting yeast cell wall mannans. J Bacteriol. 1969 Dec;100(3):1175–1181. doi: 10.1128/jb.100.3.1175-1181.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mahadevan P. R., Tatum E. L. Localization of structural polymers in the cell wall of Neurospora crassa. J Cell Biol. 1967 Nov;35(2):295–302. doi: 10.1083/jcb.35.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mahadevan P. R., Tatum E. L. Relationship of the major constituents of the Neurospora crassa cell wall to wild-type and colonial morphology. J Bacteriol. 1965 Oct;90(4):1073–1081. doi: 10.1128/jb.90.4.1073-1081.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mishra N. C. Genetics and biochemistry of morphogenesis in Neurospora. Adv Genet. 1977;19:341–405. doi: 10.1016/s0065-2660(08)60248-5. [DOI] [PubMed] [Google Scholar]
- Sandford P. A., Conrad H. E. The structure of the Aerobacter aerogenes A3(S1) polysaccharide. I. A reexamination using improved procedures for methylation analysis. Biochemistry. 1966 May;5(5):1508–1517. doi: 10.1021/bi00869a009. [DOI] [PubMed] [Google Scholar]
- Talmadge K. W., Keegstra K., Bauer W. D., Albersheim P. The Structure of Plant Cell Walls: I. The Macromolecular Components of the Walls of Suspension-cultured Sycamore Cells with a Detailed Analysis of the Pectic Polysaccharides. Plant Physiol. 1973 Jan;51(1):158–173. doi: 10.1104/pp.51.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wrathall C. R., Tatum E. L. Hyphal wall peptides and colonial morphology in Neurospora crassa. Biochem Genet. 1974 Jul;12(1):59–68. doi: 10.1007/BF00487528. [DOI] [PubMed] [Google Scholar]
- Wrathall C. R., Tatum E. L. The peptides of the hyphal wall of Neurospora crassa. J Gen Microbiol. 1973 Sep;78(1):139–153. doi: 10.1099/00221287-78-1-139. [DOI] [PubMed] [Google Scholar]