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. 1979 Feb;137(2):746–751. doi: 10.1128/jb.137.2.746-751.1979

Variation in the structure and bacteriophage-inactivating capacity of Salmonella anatum lipopolysaccharide as a function of growth temperature.

M McConnell, A Wright
PMCID: PMC218352  PMID: 422511

Abstract

Growth temperature affects both the structure and the phage-inactivating capacity of Salmonella anatum A1 lipopolysaccharide. Whereas S. anatum cells normally synthesize smooth lipopolysaccharide when grown at physiological temperature (37 degrees C), a partial smooth-rough transition occurs when cells are grown at low temperature (20 to 25 degrees C). The synthesis at low growth temperature of lipopolysaccharide molecules lacking O-antigen was detected both by increased sensitivity of cells to the rough-specific bacteriophage Felix O-1 and by fractionation of oligosaccharides derived from lipopolysaccharide by mild acid hydrolysis. Growth temperature-induced changes in the structure of S. anatum A1 lipopolysaccharide also affected its ability to inactivate epsilon15, a bacteriophage that binds initially to the O-antigen portion of the molecule. Purified lipopolysaccharide prepared from cells grown at low growth temperature exhibited a higher in vitro phage-inactivating capacity than did lipopolysaccharide prepared from cells grown at physiological temperature (37 degrees C).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. doi: 10.1042/bj0810384. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BECKMANN I., SUBBAIAH T. V., STOCKER B. A. ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM. II. SEROLOGICAL AND CHEMICAL INVESTIGATIONS. Nature. 1964 Mar 28;201:1299–1301. doi: 10.1038/2011299a0. [DOI] [PubMed] [Google Scholar]
  3. DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
  4. Dröge W., Lehmann V., Lüderitz O., Westphal O. Structural investigations on the 2-keto-3-deoxyoctonate region of lipopolysaccharides. Eur J Biochem. 1970 May 1;14(1):175–184. doi: 10.1111/j.1432-1033.1970.tb00276.x. [DOI] [PubMed] [Google Scholar]
  5. GHUYSEN J. M., STROMINGER J. L. STRUCTURE OF THE CELL WALL OF STAPHYLOCOCCUS AUREUS, STRAIN COPENHAGEN. I. PREPARATION OF FRAGMENTS BY ENZYMATIC HYDROLYSIS. Biochemistry. 1963 Sep-Oct;2:1110–1119. doi: 10.1021/bi00905a035. [DOI] [PubMed] [Google Scholar]
  6. Kent J. L., Osborn M. J. Properties of the O-specific hapten formed in vivo by mutant strains of Salmonella typhimurium. Biochemistry. 1968 Dec;7(12):4396–4408. doi: 10.1021/bi00852a036. [DOI] [PubMed] [Google Scholar]
  7. Khesin R. B., Mindlin S. Z., Gorlenko Z. M., Ilyina T. S. Temperature sensitive mutations affecting RNA synthesis in Escherichia coli. Mol Gen Genet. 1968;103(2):194–208. doi: 10.1007/BF00427146. [DOI] [PubMed] [Google Scholar]
  8. Koeltzow D. E., Conrad H. E. Structural heterogeneity in the lipopolysaccharide of Aerobacter aerogenes NCTC 243. Biochemistry. 1971 Jan 19;10(2):214–224. doi: 10.1021/bi00778a004. [DOI] [PubMed] [Google Scholar]
  9. Lindberg A. A., Holme T. Influence of O side chains on the attachment of the Felix O-1 bacteriophage to Salmonella bacteria. J Bacteriol. 1969 Aug;99(2):513–519. doi: 10.1128/jb.99.2.513-519.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mühlradt P. F., Golecki J. R. Asymmetrical distribution and artifactual reorientation of lipopolysaccharide in the outer membrane bilayer of Salmonella typhimurium. Eur J Biochem. 1975 Feb 21;51(2):343–352. doi: 10.1111/j.1432-1033.1975.tb03934.x. [DOI] [PubMed] [Google Scholar]
  11. Nikaido H. Structure of cell wall lipopolysaccharide from Salmonella typhimurium. Further studies on the linkage between O side chains and R core. Eur J Biochem. 1970 Jul;15(1):57–62. doi: 10.1111/j.1432-1033.1970.tb00975.x. [DOI] [PubMed] [Google Scholar]
  12. Osborn M. J., Gander J. E., Parisi E. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Site of synthesis of lipopolysaccharide. J Biol Chem. 1972 Jun 25;247(12):3973–3986. [PubMed] [Google Scholar]
  13. ROBBINS P. W., UCHIDA T. Studies on the chemical basis of the phage conversion of O-antigens in the E-group Salmonellae. Biochemistry. 1962 Mar;1:323–335. doi: 10.1021/bi00908a020. [DOI] [PubMed] [Google Scholar]
  14. Shands J. W. Localization of somatic antigen on gram-negative bacteria using ferritin antibody conjugates. Ann N Y Acad Sci. 1966 Jun 30;133(2):292–298. doi: 10.1111/j.1749-6632.1966.tb52372.x. [DOI] [PubMed] [Google Scholar]
  15. Smit J., Kamio Y., Nikaido H. Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants. J Bacteriol. 1975 Nov;124(2):942–958. doi: 10.1128/jb.124.2.942-958.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Takeda K., Uetake H. In vitro interaction between phage and receptor lipopolysaccharide: a novel glycosidase associated with Salmonella phage epsilon15. Virology. 1973 Mar;52(1):148–159. [PubMed] [Google Scholar]
  17. Weiner I. M., Higuchi T., Rothfield L., Saltmarsh-Andrew M., Osborn M. J., Horecker B. L. Biosynthesis of bacterial lipopolysaccharide. V. Lipid-linked intermediates in the biosynthesis of the O-antigen groups of Salmonella typhimurium. Proc Natl Acad Sci U S A. 1965 Jul;54(1):228–235. doi: 10.1073/pnas.54.1.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wright A., Barzilai N. Isolation and haracterization nonconverting mutants of bacteriophage epsilon 34. J Bacteriol. 1971 Mar;105(3):937–939. doi: 10.1128/jb.105.3.937-939.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wright A., Dankert M., Robbins P. W. Evidence for an intermediate stage in the biosynthesis of the Salmonella O-antigen. Proc Natl Acad Sci U S A. 1965 Jul;54(1):235–241. doi: 10.1073/pnas.54.1.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wright A., Kanegasaki S. Molecular aspects of lipopolysaccharides. Physiol Rev. 1971 Oct;51(4):748–784. doi: 10.1152/physrev.1971.51.4.748. [DOI] [PubMed] [Google Scholar]
  21. Wright A. Mechanism of conversion of the salmonella O antigen by bacteriophageepsilon 34. J Bacteriol. 1971 Mar;105(3):927–936. doi: 10.1128/jb.105.3.927-936.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. ZINDER N. D. Lysogenization and superinfection immunity in Salmonella. Virology. 1958 Apr;5(2):291–326. doi: 10.1016/0042-6822(58)90025-4. [DOI] [PubMed] [Google Scholar]

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