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. 1978 Apr 1;147(4):1253–1266. doi: 10.1084/jem.147.4.1253

Proliferative capacity of mouse peritoneal macrophages in vitro

PMCID: PMC2184262  PMID: 650153

Abstract

Thioglycolate-stimulated mouse peritoneal macrophages cultured in the presence of macrophage growth factor (MGF) will continue to proliferate when they are removed from culture dishes with the local anesthetic lidocaine and subcultured. The number of times the cells can be subcultured and remain in a proliferative state is dependent on the number of previous cell divisions. One precursor cell (colony-forming cell) yields about 2.6 X 10(4) daughter cells. When MGF is removed from actively proliferating macrophages, they leave the cell cycle and enter a "resting" condition. When MGF is readded, cells reenter the cell cycle and proliferate with the same doubling time as if MGF had not been removed. Membrane 5'-nucleotidase activity was used as a probe to identify the state of macrophage activation. Proliferating macrophage populations had significantly higher enzyme levels than stimulated macrophages cultured without MGF. These enzymes levels were, however, lower than those found for resident (unstimulated) macrophages.

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Selected References

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  1. Anderson L. E., McClure W. O. An improved scintillation cocktail of high-solubilizing power. Anal Biochem. 1973 Jan;51(1):173–179. doi: 10.1016/0003-2697(73)90465-x. [DOI] [PubMed] [Google Scholar]
  2. Austin P. E., McCulloch E. A., Till J. E. Characterization of the factor in L-cell conditioned medium capable of stimulating colony formation by mouse marrow cells in culture. J Cell Physiol. 1971 Apr;77(2):121–134. doi: 10.1002/jcp.1040770202. [DOI] [PubMed] [Google Scholar]
  3. Bianco C., Eden A., Cohn Z. A. The induction of macrophage spreading: role of coagulation factors and the complement system. J Exp Med. 1976 Dec 1;144(6):1531–1544. doi: 10.1084/jem.144.6.1531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bianco C., Griffin F. M., Jr, Silverstein S. C. Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation. J Exp Med. 1975 Jun 1;141(6):1278–1290. doi: 10.1084/jem.141.6.1278. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chui D. H., Djaldetti M., Marks P. A., Rifkind R. A. Erythropoietin effects on fetal mouse erythroid cells. I. Cell population and hemoglobin synthesis. J Cell Biol. 1971 Dec;51(3):585–595. doi: 10.1083/jcb.51.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edelson P. J., Cohn Z. A. 5'-Nucleotidase activity of mouse peritoneal macrophages. I. Synthesis and degradation in resident and inflammatory populations. J Exp Med. 1976 Dec 1;144(6):1581–1595. doi: 10.1084/jem.144.6.1581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Edelson P. J., Zwiebel R., Cohn Z. A. The pinocytic rate of activated macrophages. J Exp Med. 1975 Nov 1;142(5):1150–1164. doi: 10.1084/jem.142.5.1150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gordon S., Unkeless J. C., Cohn Z. A. Induction of macrophage plasminogen activator by endotoxin stimulation and phagocytosis: evidence for a two-stage process. J Exp Med. 1974 Oct 1;140(4):995–1010. doi: 10.1084/jem.140.4.995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Lin H. S., Stewart C. C. Peritoneal exudate cells. I. Growth requirement of cells capable of forming colonies in soft agar. J Cell Physiol. 1974 Jun;83(3):369–378. doi: 10.1002/jcp.1040830307. [DOI] [PubMed] [Google Scholar]
  11. Metcalf D. Regulation of granulocyte and monocyte-macrophage proliferation by colony stimulating factor (CSF): a review. Exp Hematol. 1973;1(4):185–201. [PubMed] [Google Scholar]
  12. Munakata N., Strauss B. Continued proliferation of mitogen-stimulated human peripheral blood lymphocytes: requirement for the restimulation of progeny. Cell Immunol. 1972 Jul;4(3):243–255. doi: 10.1016/0008-8749(72)90028-7. [DOI] [PubMed] [Google Scholar]
  13. Rabinovitch M., DeStefano M. J. Cell shape changes induced by cationic anesthetics. J Exp Med. 1976 Feb 1;143(2):290–304. doi: 10.1084/jem.143.2.290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rabinovitch M., DeStefano M. J. Use of the local anesthetic lidocaine for cell harvesting and subcultivation. In Vitro. 1975 Nov-Dec;11(6):379–381. doi: 10.1007/BF02616374. [DOI] [PubMed] [Google Scholar]
  15. Stanley E. R., Cifone M., Heard P. M., Defendi V. Factors regulating macrophage production and growth: identity of colony-stimulating factor and macrophage growth factor. J Exp Med. 1976 Mar 1;143(3):631–647. doi: 10.1084/jem.143.3.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stewart C. C., Cramer S. F., Steward P. G. The response of human peripheral blood lymphocytes to phytohemagglutinin: determination of cell numbers. Cell Immunol. 1975 Apr;16(2):237–250. doi: 10.1016/0008-8749(75)90115-x. [DOI] [PubMed] [Google Scholar]
  17. Stewart C. C., Lin H. S., Adles C. Proliferation and colony-forming ability of peritoneal exudate cells in liquid culture. J Exp Med. 1975 May 1;141(5):1114–1132. doi: 10.1084/jem.141.5.1114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stewart C. C. Nutrient utilization by peritoneal exudate cells. J Reticuloendothel Soc. 1973 Oct;14(4):332–349. [PubMed] [Google Scholar]
  19. Unkeless J. C., Gordon S., Reich E. Secretion of plasminogen activator by stimulated macrophages. J Exp Med. 1974 Apr 1;139(4):834–850. doi: 10.1084/jem.139.4.834. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. VOLKMAN A., GOWANS J. L. THE ORIGIN OF MACROPHAGES FROM BONE MARROW IN THE RAT. Br J Exp Pathol. 1965 Feb;46:62–70. [PMC free article] [PubMed] [Google Scholar]
  21. VOLKMAN A., GOWANS J. L. THE PRODUCTION OF MACROPHAGES IN THE RAT. Br J Exp Pathol. 1965 Feb;46:50–61. [PMC free article] [PubMed] [Google Scholar]
  22. Van Furth R., Diesselhoff-den Dulk M. C., Mattie H. Quantitative study on the production and kinetics of mononuclear phagocytes during an acute inflammatory reaction. J Exp Med. 1973 Dec 1;138(6):1314–1330. doi: 10.1084/jem.138.6.1314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vassalli J. D., Hamilton J., Reich E. Macrophage plasminogen activator: modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors, and cyclic nucleotides. Cell. 1976 Jun;8(2):271–281. doi: 10.1016/0092-8674(76)90011-8. [DOI] [PubMed] [Google Scholar]
  24. Vassalli J. D., Reich E. Macrophage plasminogen activator: induction by products of activated lymphoid cells. J Exp Med. 1977 Feb 1;145(2):429–437. doi: 10.1084/jem.145.2.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Virolainen M., Defendi V. Dependence of macrophage growth in vitro upon interaction with other cell types. Wistar Inst Symp Monogr. 1967;7:67–85. [PubMed] [Google Scholar]
  26. Werb Z., Gordon S. Elastase secretion by stimulated macrophages. Characterization and regulation. J Exp Med. 1975 Aug 1;142(2):361–377. doi: 10.1084/jem.142.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Werb Z., Gordon S. Secretion of a specific collagenase by stimulated macrophages. J Exp Med. 1975 Aug 1;142(2):346–360. doi: 10.1084/jem.142.2.346. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. White R., Lin H. S., Kuhn C., 3rd Elastase secretion by peritoneal exudative and alveolar macrophages. J Exp Med. 1977 Sep 1;146(3):802–808. doi: 10.1084/jem.146.3.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. van Furth R., Cohn Z. A., Hirsch J. G., Humphrey J. H., Spector W. G., Langevoort H. L. The mononuclear phagocyte system: a new classification of macrophages, monocytes, and their precursor cells. Bull World Health Organ. 1972;46(6):845–852. [PMC free article] [PubMed] [Google Scholar]
  30. van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

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