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. 1979 Jan;137(1):226–233. doi: 10.1128/jb.137.1.226-233.1979

Inactivation of bacteriophages by protein E, a new major membrane protein isolated from an Escherichia coli mutant.

T J Chai, J Foulds
PMCID: PMC218440  PMID: 368014

Abstract

Pure protein E, obtained after diethylaminoethyl-cellulose chromatography of ethylenediaminetetraacetic acid-Triton X-100-solubilized outer membrane proteins of Escherichia coli strain JF694, inactivated bacteriophage K3. Lipopolysaccharide enhanced bacteriophage inactivation. Antibody prepared against purified protein E protected bacteriophage K3 from inactivation by protein E. Bacteriophage K3 used a major outer membrane protein, protein II*, as part of its receptor. We conclude that proteins E and II* have a common region which interacts with bacteriophage K3. Protein E also inactivated two recently described bacteriophages, TC45 and TC23, that use protein E as at least part of their receptor.

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Selected References

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