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. 1978 Dec;136(3):1159–1164. doi: 10.1128/jb.136.3.1159-1164.1978

An explanation for the apparent host specificity of Pseudomonas plasmid R91 expression.

G A Jacoby, R Weiss, T R Korfhagen, V Krishnapillai, A E Jacob, R W Hedges
PMCID: PMC218551  PMID: 102634

Abstract

Pseudomonas aeruginosa strain 9169 has been reported to contain a plasmid that expresses resistance to carbenicillin (Cb), kanamycin (Km), and tetracycline (Tc) in Escherichia coli but resistance only to Cb in certain Pseudomonas recipients. The triply resistant plasmid in E. coli belonged to incompatibility (Inc) group P or P-1, whereas the singly resistant plasmid in P. aeruginosa was compatible with IncP-1 plasmids and other plasmids of established Inc specificity but incompatible with plasmid pSR1 that is here used to define a new Pseudomonas Inc group P-10. Additional physical and genetic studies showed that strain 9169 contained not one but two plasmids: IncP-1 plasmid R91a, determining the Cb Km Tc phenotype, and IncP-10 plasmid R91, determining Cb that differed in molecular weight and in EcoRI and BamHI restriction endonuclease recognition sites. Plasmid multiplicity rather than host effects on plasmid gene expression can account for differences in the phenotype of strain 9169 transconjugants to E. coli and P. aeruginosa.

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Selected References

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  1. Barth P. T., Datta N., Hedges R. W., Grinter N. J. Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistances from R483 to other replicons. J Bacteriol. 1976 Mar;125(3):800–810. doi: 10.1128/jb.125.3.800-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barth P. T., Grinter N. J. Map of plasmid RP4 derived by insertion of transposon C. J Mol Biol. 1977 Jul 5;113(3):455–474. doi: 10.1016/0022-2836(77)90233-9. [DOI] [PubMed] [Google Scholar]
  3. Burkardt H. J., Mattes R., Pühler A., Heumann W. Electron microscopy and computerized evaluation of some partially denatured group P resistance plasmids. J Gen Microbiol. 1978 Mar;105(1):51–62. doi: 10.1099/00221287-105-1-51. [DOI] [PubMed] [Google Scholar]
  4. Chandler P. M., Krishnapillai V. Characterization of Pseudomonas aeruginosa derepressed R-plasmids. J Bacteriol. 1977 May;130(2):596–603. doi: 10.1128/jb.130.2.596-603.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chandler P. M., Krishnapillai V. Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors readily transferable between Pseudomonas and the Enterobacteriaceae. Genet Res. 1974 Jun;23(3):239–250. doi: 10.1017/s0016672300014890. [DOI] [PubMed] [Google Scholar]
  6. Chandler P. M., Krishnapillai V. Phenotypic properties of R factors of Pseudomonas aeruginosa: R factors transferable only in Pseudomonas aeruginosa. Genet Res. 1974 Jun;23(3):251–257. doi: 10.1017/s0016672300014907. [DOI] [PubMed] [Google Scholar]
  7. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Coetzee J. N., Datta N., Hedges R. W. R factors from Proteus rettgeri. J Gen Microbiol. 1972 Oct;72(3):543–552. doi: 10.1099/00221287-72-3-543. [DOI] [PubMed] [Google Scholar]
  9. Hedges R. W., Jacob A. E. Mobilization of plasmid-borne drug resistance determinants for transfer from Pseudomonas aeruginosa to Escherichia coli. Mol Gen Genet. 1975 Sep 15;140(1):69–79. doi: 10.1007/BF00268990. [DOI] [PubMed] [Google Scholar]
  10. Hedges R. W., Jacob A. E. Transposition of ampicillin resistance from RP4 to other replicons. Mol Gen Genet. 1974;132(1):31–40. doi: 10.1007/BF00268228. [DOI] [PubMed] [Google Scholar]
  11. Holloway B. W. Genetics of Pseudomonas. Bacteriol Rev. 1969 Sep;33(3):419–443. doi: 10.1128/br.33.3.419-443.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ingram L., Sykes R. B., Grinsted J., Saunders J. R., Richmond M. H. A transmissible resistance element from a strain of Pseudomonas aeruginosa containing no detectable extrachromosomal DNA. J Gen Microbiol. 1972 Sep;72(2):269–279. doi: 10.1099/00221287-72-2-269. [DOI] [PubMed] [Google Scholar]
  13. Jacoby G. A., Jacob A. E., Hedges R. W. Recombination between plasmids of incompatibility groups P-1 and P-2. J Bacteriol. 1976 Sep;127(3):1278–1285. doi: 10.1128/jb.127.3.1278-1285.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Jacoby G. A. Properties of R plasmids determining gentamicin resistance by acetylation in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1974 Sep;6(3):239–252. doi: 10.1128/aac.6.3.239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Korfhagen T. R., Loper J. C., Ferrel J. A. Pseudomonas aeruginosa R factors determining gentamicin plus carbenicillin resistance from patients with urinary tract colonizations. Antimicrob Agents Chemother. 1975 Jan;7(1):64–68. doi: 10.1128/aac.7.1.64. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Krishnapillai V. Superinfection inhibition by prophage B3 of some R plasmids in Pseudomonas aeruginosa. Genet Res. 1977 Feb;29(1):47–54. doi: 10.1017/s0016672300017109. [DOI] [PubMed] [Google Scholar]
  17. Krishnapillai V. The use of bacteriophages for differentiating plasmids of Pseudomonas aeruginosa. Genet Res. 1974 Jun;23(3):327–334. doi: 10.1017/s0016672300014968. [DOI] [PubMed] [Google Scholar]
  18. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  19. Lederberg E. M., Cohen S. N. Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid. J Bacteriol. 1974 Sep;119(3):1072–1074. doi: 10.1128/jb.119.3.1072-1074.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lowbury E. J., Lilly H. A., Kidson A., Ayliffe G. A., Jones R. J. Sensitivity of Pseudomonas aeruginosa to antibiotics: emergence of strains highly resistant to carbenicillin. Lancet. 1969 Aug 30;2(7618):448–452. doi: 10.1016/s0140-6736(69)90163-9. [DOI] [PubMed] [Google Scholar]
  21. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Olsen R. H., Siak J. S., Gray R. H. Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage. J Virol. 1974 Sep;14(3):689–699. doi: 10.1128/jvi.14.3.689-699.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Olsen R. H., Thomas D. D. Characteristics and purification of PRR1, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid. J Virol. 1973 Dec;12(6):1560–1567. doi: 10.1128/jvi.12.6.1560-1567.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Skurray R. A., Guyer M. S., Timmis K., Cabello F., Cohen S. N., Davidson N., Clark A. J. Replication region fragments cloned from Flac+ are identical to EcoRI fragment f5 of F. J Bacteriol. 1976 Sep;127(3):1571–1575. doi: 10.1128/jb.127.3.1571-1575.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Stanisich V. A., Holloway B. W. Chromosome transfer in Pseudomonas aeruginosa mediated by R factors. Genet Res. 1971 Apr;17(2):169–172. doi: 10.1017/s0016672300012179. [DOI] [PubMed] [Google Scholar]
  26. Stanisich V. A. The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. J Gen Microbiol. 1974 Oct;84(2):332–342. doi: 10.1099/00221287-84-2-332. [DOI] [PubMed] [Google Scholar]
  27. Tanaka T., Weisblum B. Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid. J Bacteriol. 1975 Jan;121(1):354–362. doi: 10.1128/jb.121.1.354-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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