Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 May;162(2):830–832. doi: 10.1128/jb.162.2.830-832.1985

Novel dnaG mutation in a dnaP mutant of Escherichia coli.

Y Murakami, T Nagata, W Schwarz, C Wada, T Yura
PMCID: PMC218929  PMID: 3886637

Abstract

Reexamination of the dnaP18 mutant strain of Escherichia coli revealed that the mutation responsible for the arrest of DNA replication and cell growth at high temperatures resides in the dnaG gene rather than in the dnaP locus as previously thought; this mutation has been designated dnaG2903.

Full text

PDF
830

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carl P. L. Escherichia coli mutants with temperature-sensitive synthesis of DNA. Mol Gen Genet. 1970;109(2):107–122. doi: 10.1007/BF00269647. [DOI] [PubMed] [Google Scholar]
  3. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gross J. D. DNA replication in bacteria. Curr Top Microbiol Immunol. 1972;57:39–74. doi: 10.1007/978-3-642-65297-4_2. [DOI] [PubMed] [Google Scholar]
  5. Harris J. D., Heilig J. S., Martinez I. I., Calendar R., Isaksson L. A. Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6177–6181. doi: 10.1073/pnas.75.12.6177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Horiuchi T., Nagata T. Mutations affecting growth of the Escherichia coli cell under a condition of DNA polymerase I-deficiency. Mol Gen Genet. 1973;123(1):89–110. doi: 10.1007/BF00282992. [DOI] [PubMed] [Google Scholar]
  7. Kleckner N., Barker D. F., Ross D. G., Botstein D. Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda. Genetics. 1978 Nov;90(3):427–461. doi: 10.1093/genetics/90.3.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Low K. B. Escherichia coli K-12 F-prime factors, old and new. Bacteriol Rev. 1972 Dec;36(4):587–607. doi: 10.1128/br.36.4.587-607.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Marinus M. G., Adelberg E. A. Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12. J Bacteriol. 1970 Dec;104(3):1266–1272. doi: 10.1128/jb.104.3.1266-1272.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nakamura Y. Amber dnaG mutation exerting a polar effect on the synthesis of RNA polymerase sigma factor in Escherichia coli. Mol Gen Genet. 1984;196(1):179–182. doi: 10.1007/BF00334114. [DOI] [PubMed] [Google Scholar]
  12. Nakamura Y. Hybrid plasmid carrying Escherichia coli genes for the primase (dnaG) and RNA polymerase sigma factor (rpoD); gene organization and control of their expression. Mol Gen Genet. 1980;178(3):487–497. doi: 10.1007/BF00337853. [DOI] [PubMed] [Google Scholar]
  13. Nakamura Y., Osawa T., Yura T. Intragenic localization of amber and temperature-sensitive rpoD mutations affecting RNA polymerase sigma factor of Escherichia coli. Mol Gen Genet. 1983;189(2):193–198. doi: 10.1007/BF00337803. [DOI] [PubMed] [Google Scholar]
  14. Osawa T., Yura T. Amber mutations in the structural gene for RNA polymerase sigma factor of Escherichia coli. Mol Gen Genet. 1980;180(2):293–300. doi: 10.1007/BF00425841. [DOI] [PubMed] [Google Scholar]
  15. Travers A. A., Buckland R., Goman M., Le Grice S. S., Scaife J. G. A mutation affecting the sigma subunit of RNA polymerase changes transcriptional specificity. Nature. 1978 Jun 1;273(5661):354–358. doi: 10.1038/273354a0. [DOI] [PubMed] [Google Scholar]
  16. Wada C., Yura T. Phenethyl alcohol resistance in Escherichia coli. 3. A temperature-sensitive mutation(dnaP) affecting DNA replication. Genetics. 1974 Jun;77(2):199–220. doi: 10.1093/genetics/77.2.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wechsler J. A., Gross J. D. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol Gen Genet. 1971;113(3):273–284. doi: 10.1007/BF00339547. [DOI] [PubMed] [Google Scholar]
  18. Wilkins B. M., Boulnois G. J., Lanka E. A plasmid DNA primase active in discontinuous bacterial DNA replication. Nature. 1981 Mar 19;290(5803):217–221. doi: 10.1038/290217a0. [DOI] [PubMed] [Google Scholar]
  19. Wilkins B. M. Partial suppression of the phenotype of Escherichia coli K-12 dnaG mutants by some I-like conjugative plasmids. J Bacteriol. 1975 Jun;122(3):899–904. doi: 10.1128/jb.122.3.899-904.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wold M. S., McMacken R. Regulation of expression of the Escherichia coli dnaG gene and amplification of the dnaG primase. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4907–4911. doi: 10.1073/pnas.79.16.4907. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES