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. 1985 Apr;162(1):106–109. doi: 10.1128/jb.162.1.106-109.1985

Escherichia coli mutants possessing an Li+-resistant melibiose carrier.

S Shiota, Y Yamane, M Futai, T Tsuchiya
PMCID: PMC218961  PMID: 3884586

Abstract

Escherichia coli K-12 strains in the absence of the lactose carrier grew on the disaccharide melibiose as the sole source of carbon. The presence of 0.1 mM Li+ in the medium strongly inhibited growth of such cells, and Li+-resistant mutants appeared after several days of incubation. These mutants showed altered cation coupling to melibiose transport via the melibiose carrier. Cotransport between H+ and melibiose was lost in the mutants, although Na+-melibiose cotransport was retained. We observed no Li+-melibiose cotransport. Therefore, these mutants represent a new type of cation-coupling mutants of the melibiose carrier.

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Selected References

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  1. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  2. Lopilato J., Tsuchiya T., Wilson T. H. Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli. J Bacteriol. 1978 Apr;134(1):147–156. doi: 10.1128/jb.134.1.147-156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Niiya S., Yamasaki K., Wilson T. H., Tsuchiya T. Altered cation coupling to melibiose transport in mutants of Escherichia coli. J Biol Chem. 1982 Aug 10;257(15):8902–8906. [PubMed] [Google Scholar]
  4. Schuldiner S., Fishkes H. Sodium-proton antiport in isolated membrane vesicles of Escherichia coli. Biochemistry. 1978 Feb 21;17(4):706–711. doi: 10.1021/bi00597a023. [DOI] [PubMed] [Google Scholar]
  5. Shiota S., Yazyu H., Tsuchiya T. Escherichia coli mutants with altered cation recognition by the melibiose carrier. J Bacteriol. 1984 Oct;160(1):445–447. doi: 10.1128/jb.160.1.445-447.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Stock J., Roseman S. A sodium-dependent sugar co-transport system in bacteria. Biochem Biophys Res Commun. 1971 Jul 2;44(1):132–138. doi: 10.1016/s0006-291x(71)80168-7. [DOI] [PubMed] [Google Scholar]
  7. Tanaka K., Niiya S., Tsuchiya T. Melibiose transport of Escherichia coli. J Bacteriol. 1980 Mar;141(3):1031–1036. doi: 10.1128/jb.141.3.1031-1036.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Tanaka S., Lerner S. A., Lin E. C. Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol. J Bacteriol. 1967 Feb;93(2):642–648. doi: 10.1128/jb.93.2.642-648.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tsuchiya T., Lopilato J., Wilson T. H. Effect of lithium ion on melibiose transport in Escherichia coli. J Membr Biol. 1978 Jul 21;42(1):45–59. doi: 10.1007/BF01870393. [DOI] [PubMed] [Google Scholar]
  10. Tsuchiya T., Oho M., Shiota-Niiya S. Lithium ion-sugar cotransport via the melibiose transport system in Escherichia coli. Measurement of Li+ transport and specificity. J Biol Chem. 1983 Nov 10;258(21):12765–12767. [PubMed] [Google Scholar]
  11. Tsuchiya T., Raven J., Wilson T. H. Co-transport of Na+ and methul-beta-D-thiogalactopyranoside mediated by the melibiose transport system of Escherichia coli. Biochem Biophys Res Commun. 1977 May 9;76(1):26–31. doi: 10.1016/0006-291x(77)91663-1. [DOI] [PubMed] [Google Scholar]
  12. Tsuchiya T., Wilson T. H. Cation-sugar cotransport in the melibiose transport system of Escherichia coli. Membr Biochem. 1978;2(1):63–79. doi: 10.3109/09687687809063858. [DOI] [PubMed] [Google Scholar]
  13. Umeda K., Shiota S., Futai M., Tsuchiya T. Inhibitory effect of Li+ on cell growth and pyruvate kinase activity of Escherichia coli. J Bacteriol. 1984 Nov;160(2):812–814. doi: 10.1128/jb.160.2.812-814.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. West I. C., Wilson T. H. Galactoside transport dissociated from proton movement in mutants of Escherichia coli. Biochem Biophys Res Commun. 1973 Jan 23;50(2):551–558. doi: 10.1016/0006-291x(73)90875-9. [DOI] [PubMed] [Google Scholar]
  15. Wilson T. H., Kusch M. A mutant of Escherichia coli K 12 energy-uncoupled for lactose transport. Biochim Biophys Acta. 1972 Mar 17;255(3):786–797. doi: 10.1016/0005-2736(72)90391-4. [DOI] [PubMed] [Google Scholar]
  16. Yazyu H., Shiota-Niiya S., Shimamoto T., Kanazawa H., Futai M., Tsuchiya T. Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. J Biol Chem. 1984 Apr 10;259(7):4320–4326. [PubMed] [Google Scholar]

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