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. 1985 Apr;162(1):280–285. doi: 10.1128/jb.162.1.280-285.1985

Cloning of the Escherichia coli recJ chromosomal region and identification of its encoded proteins.

S T Lovett, A J Clark
PMCID: PMC218986  PMID: 2984175

Abstract

A 9.6-kilobase BamHI-SalI fragment carrying recJ+ was cloned into vector pBR322. Deletion and transposon mutagenesis were used to map the recJ gene on this fragment. The maxicell protein-labeling technique was used to correlate a functional recJ gene with the presence of a polypeptide of 53,000 apparent molecular weight. Two additional genes, one encoding two proteins of 26,000 and 25,000 Mr and the other encoding a 31,000-Mr protein, were mapped on a 3.7-kilobase HindIII-SalI subfragment with recJ. Functions for these adjacent genes are not known; however, insertion mutations in these genes lessen the expression of the putative recJ protein detected in maxicells. A 9.6-kilobase BamHI-SalI fragment carrying the temperature-sensitive mutation recJ147 was also cloned and used for complementation studies to identify other recJ mutations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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