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. 1985 Apr;162(1):307–316. doi: 10.1128/jb.162.1.307-316.1985

Composite IS1 elements encoding hydroxamate-mediated iron uptake in FIme plasmids from epidemic Salmonella spp.

B Colonna, M Nicoletti, P Visca, M Casalino, P Valenti, F Maimone
PMCID: PMC218990  PMID: 2984176

Abstract

Eleven FIme plasmids representative of those identified in epidemic strains of Salmonella wien and Salmonella typhimurium isolated in North Africa, Europe, and the Middle East have been examined for the presence of determinants of toxigenicity, adherence, and iron-sequestering mechanisms. Chemical and genetic data indicated that all plasmids code for a hydroxamate-mediated iron assimilation system. Detailed analysis of derivative plasmids and cloned fragments of FIme plasmid pZM61 demonstrated that the general genetic and structural organization of the DNA region containing the genes for hydroxamate biosynthesis and cloacin DF13 receptor was virtually identical to that described for the aerobactin-mediated iron uptake system of pColV-K30. This DNA region is part of a composite element that is 16.7 kilobases long and carries its IS1 modules as inverted repeats. A very similar element is present in either orientation in all nine FIme plasmids analyzed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson E. S., Threlfall E. J., Carr J. M., McConnell M. M., Smith H. R. Clonal distribution of resistance plasmid-carrying Salmonella typhimurium, mainly in the Middle East. J Hyg (Lond) 1977 Dec;79(3):425–448. doi: 10.1017/s0022172400053286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Atkin C. L., Neilands J. B., Phaff H. J. Rhodotorulic acid from species of Leucosporidium, Rhodosporidium, Rhodotorula, Sporidiobolus, and Sporobolomyces, and a new alanine-containing ferrichrome from Cryptococcus melibiosum. J Bacteriol. 1970 Sep;103(3):722–733. doi: 10.1128/jb.103.3.722-733.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bindereif A., Neilands J. B. Cloning of the aerobactin-mediated iron assimilation system of plasmid ColV. J Bacteriol. 1983 Feb;153(2):1111–1113. doi: 10.1128/jb.153.2.1111-1113.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Casalino M., Comanducci A., Nicoletti M., Maimone F. Stability of plasmid content in Salmonella wien in late phases of the epidemic history. Antimicrob Agents Chemother. 1984 Apr;25(4):499–501. doi: 10.1128/aac.25.4.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chau P. Y., Ling J., Threlfall E. J., Im S. W. Genetic instability of R plasmids in relation to the shift of drug resistance patterns in Salmonella johannesburg. J Gen Microbiol. 1982 Feb;128(2):239–245. doi: 10.1099/00221287-128-2-239. [DOI] [PubMed] [Google Scholar]
  6. Darfeuille A., Lafeuille B., Joly B., Cluzel R. A new colonization factor antigen (CFA/III) produced by enteropathogenic Escherichia coli O128:B12. Ann Microbiol (Paris) 1983 Jan-Feb;134A(1):53–64. doi: 10.1016/0769-2609(83)90103-5. [DOI] [PubMed] [Google Scholar]
  7. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu. J Infect Dis. 1972 Apr;125(4):407–411. doi: 10.1093/infdis/125.4.407. [DOI] [PubMed] [Google Scholar]
  8. Evans D. J., Jr, Evans D. G., DuPont H. L. Hemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken, and guinea pig erythrocytes in the presence and absence of mannose. Infect Immun. 1979 Feb;23(2):336–346. doi: 10.1128/iai.23.2.336-346.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Finkelstein R. A., Sciortino C. V., McIntosh M. A. Role of iron in microbe-host interactions. Rev Infect Dis. 1983 Sep-Oct;5 (Suppl 4):S759–S777. doi: 10.1093/clinids/5.supplement_4.s759. [DOI] [PubMed] [Google Scholar]
  10. Gibson F., Magrath D. I. The isolation and characterization of a hydroxamic acid (aerobactin) formed by Aerobacter aerogenes 62-I. Biochim Biophys Acta. 1969 Nov 18;192(2):175–184. doi: 10.1016/0304-4165(69)90353-5. [DOI] [PubMed] [Google Scholar]
  11. Guerrant R. L., Brunton L. L., Schnaitman T. C., Rebhun L. I., Gilman A. G. Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli. Infect Immun. 1974 Aug;10(2):320–327. doi: 10.1128/iai.10.2.320-327.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hansen J. B., Olsen R. H. Isolation of large bacterial plasmids and characterization of the P2 incompatibility group plasmids pMG1 and pMG5. J Bacteriol. 1978 Jul;135(1):227–238. doi: 10.1128/jb.135.1.227-238.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kleckner N. Transposable elements in prokaryotes. Annu Rev Genet. 1981;15:341–404. doi: 10.1146/annurev.ge.15.120181.002013. [DOI] [PubMed] [Google Scholar]
  14. Krone W. J., Oudega B., Stegehuis F., de Graaf F. K. Cloning and expression of the cloacin DF13/aerobactin receptor of Escherichia coli (ColV-K30). J Bacteriol. 1983 Feb;153(2):716–721. doi: 10.1128/jb.153.2.716-721.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lawlor K. M., Payne S. M. Aerobactin genes in Shigella spp. J Bacteriol. 1984 Oct;160(1):266–272. doi: 10.1128/jb.160.1.266-272.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Maimone F., Colonna B., Bazzicalupo P., Oliva B., Nicoletti M., Casalino M. Plasmids and transposable elements in Salmonella wien. J Bacteriol. 1979 Aug;139(2):369–375. doi: 10.1128/jb.139.2.369-375.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. McDougall S., Neilands J. B. Plasmid- and chromosome-coded aerobactin synthesis in enteric bacteria: insertion sequences flank operon in plasmid-mediated systems. J Bacteriol. 1984 Jul;159(1):300–305. doi: 10.1128/jb.159.1.300-305.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Moseley S. L., Echeverria P., Seriwatana J., Tirapat C., Chaicumpa W., Sakuldaipeara T., Falkow S. Identification of enterotoxigenic Escherichia coli by colony hybridization using three enterotoxin gene probes. J Infect Dis. 1982 Jun;145(6):863–869. doi: 10.1093/infdis/145.6.863. [DOI] [PubMed] [Google Scholar]
  19. Neilands J. B. Microbial envelope proteins related to iron. Annu Rev Microbiol. 1982;36:285–309. doi: 10.1146/annurev.mi.36.100182.001441. [DOI] [PubMed] [Google Scholar]
  20. Ohtsubo H., Ohtsubo E. Nucleotide sequence of an insertion element, IS1. Proc Natl Acad Sci U S A. 1978 Feb;75(2):615–619. doi: 10.1073/pnas.75.2.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Payne S. M., Niesel D. W., Peixotto S. S., Lawlor K. M. Expression of hydroxamate and phenolate siderophores by Shigella flexneri. J Bacteriol. 1983 Sep;155(3):949–955. doi: 10.1128/jb.155.3.949-955.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Perez-Casal J. F., Crosa J. H. Aerobactin iron uptake sequences in plasmid ColV-K30 are flanked by inverted IS1-like elements and replication regions. J Bacteriol. 1984 Oct;160(1):256–265. doi: 10.1128/jb.160.1.256-265.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rangnekar V. M., Banker D. D., Jhala H. I. Antimicrobial resistance and incompatibility groups of R plasmids in Salmonella typhimurium isolated from human sources in Bombay from 1978 to 1980. Antimicrob Agents Chemother. 1983 Jan;23(1):54–58. doi: 10.1128/aac.23.1.54. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. So M., McCarthy B. J. Nucleotide sequence of the bacterial transposon Tn1681 encoding a heat-stable (ST) toxin and its identification in enterotoxigenic Escherichia coli strains. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4011–4015. doi: 10.1073/pnas.77.7.4011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  26. Speirs J. I., Stavric S., Konowalchuk J. Assay of Escherichia coli heat-labile enterotoxin with vero cells. Infect Immun. 1977 May;16(2):617–622. doi: 10.1128/iai.16.2.617-622.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Stuart S. J., Greenwood K. T., Luke R. K. Hydroxamate-mediated transport of iron controlled by ColV plasmids. J Bacteriol. 1980 Jul;143(1):35–42. doi: 10.1128/jb.143.1.35-42.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Stuart S. J., Greenwood K. T., Luke R. K. Iron-suppressible production of hydroxamate by Escherichia coli isolates. Infect Immun. 1982 Jun;36(3):870–875. doi: 10.1128/iai.36.3.870-875.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Valvano M. A., Crosa J. H. Aerobactin iron transport genes commonly encoded by certain ColV plasmids occur in the chromosome of a human invasive strain of Escherichia coli K1. Infect Immun. 1984 Oct;46(1):159–167. doi: 10.1128/iai.46.1.159-167.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Van Tiel-Menkveld G. J., Mentjox-Vervuurt J. M., Oudega B., de Graaf F. K. Siderophore production by Enterobacter cloacae and a common receptor protein for the uptake of aerobactin and cloacin DF13. J Bacteriol. 1982 May;150(2):490–497. doi: 10.1128/jb.150.2.490-497.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Warner P. J., Williams P. H., Bindereif A., Neilands J. B. ColV plasmid-specific aerobactin synthesis by invasive strains of Escherichia coli. Infect Immun. 1981 Aug;33(2):540–545. doi: 10.1128/iai.33.2.540-545.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Williams P. H. Novel iron uptake system specified by ColV plasmids: an important component in the virulence of invasive strains of Escherichia coli. Infect Immun. 1979 Dec;26(3):925–932. doi: 10.1128/iai.26.3.925-932.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Willshaw G. A., Smith H. R., Anderson E. S. Molecular studies of FIme resistance plasmids, particularly in epidemic Salmonella typhimurium. Mol Gen Genet. 1978 Feb 7;159(1):111–116. doi: 10.1007/BF00401755. [DOI] [PubMed] [Google Scholar]

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