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. 1975 Aug 1;142(2):391–402. doi: 10.1084/jem.142.2.391

Anti-immunoglobulin-induced histamine secretion by rat peritoneal mast cells studied by immunoferritin electron microscopy

PMCID: PMC2189903  PMID: 49387

Abstract

We have used ferritin-conjugated divalent and monovalent anti-Ig antibodies to study simultaneously, histamine secretion and the ultrastructural distribution and redistribution of Ig receptors on rat peritoneal mast cells. We conclude that (a) divalent anti-Ig is required for both receptor redistribution and for calcium-dependent degranulation and histamine release, (b) divalent anti-Ig induces patching and pinocytosis but not capping of Ig molecules, (c) neither capping nor pinocytosis are required for triggering and if clustering is necessary, then less than 10 Ig molecules are required per cluster, and (d) degranulation (and histamine release) is not an all or none response of the mast cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach M. K., Block K. J., Austen K. F. IgE and IgGa antibody-mediated release of histamine from rat peritoneal cells. II. Interaction of IgGa and IgE at the targe cell. J Exp Med. 1971 Apr 1;133(4):772–784. doi: 10.1084/jem.133.4.772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Becker E. L., Henson P. M. In vitro studies of immunologically induced secretion of mediators from cells and related phenomena. Adv Immunol. 1973;17:93–193. doi: 10.1016/s0065-2776(08)60732-4. [DOI] [PubMed] [Google Scholar]
  3. Becker K. E., Ishizaka T., Metzger H., Ishizaka K., Grimley P. M. Surface IgE on human basophils during histamine release. J Exp Med. 1973 Aug 1;138(2):394–409. doi: 10.1084/jem.138.2.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cochrane D. E., Douglas W. W. Calcium-induced extrusion of secretory granules (exocytosis) in mast cells exposed to 48-80 or the ionophores A-23187 and X-537A. Proc Natl Acad Sci U S A. 1974 Feb;71(2):408–412. doi: 10.1073/pnas.71.2.408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ferrarini M., Munro A., Wilson A. B. Cytophilic antibody: correlation of its distribution with activation of basophils and macrophages. Eur J Immunol. 1973 Jun;3(6):364–370. doi: 10.1002/eji.1830030609. [DOI] [PubMed] [Google Scholar]
  6. Green N. M. Electron microscopy of the immunoglobulins. Adv Immunol. 1969;11:1–30. doi: 10.1016/s0065-2776(08)60476-9. [DOI] [PubMed] [Google Scholar]
  7. Ishizaka K., Ishizaka T. Immune mechanisms of reversed type reaginic hypersensitivity. J Immunol. 1969 Sep;103(3):588–595. [PubMed] [Google Scholar]
  8. Ishizaka T., Ishizaka K., Orange R. P., Austen K. F. The capacity of human immunoglobulin E to mediate the release of histamine and slow reacting substance of anaphylaxis (SRS-A) from monkey lung. J Immunol. 1970 Feb;104(2):335–343. [PubMed] [Google Scholar]
  9. Kaliner M., Austen K. F. Cyclic AMP, ATP, and reversed anaphylactic histamine release from rat mast cells. J Immunol. 1974 Feb;112(2):664–674. [PubMed] [Google Scholar]
  10. Kanno T., Cochrane D. E., Douglas W. W. Exocytosis (secretory granule extrusion) induced by injection of calcium into mast cells. Can J Physiol Pharmacol. 1973 Dec;51(12):1001–1004. doi: 10.1139/y73-153. [DOI] [PubMed] [Google Scholar]
  11. LICHTENSTEIN L. M., OSLER A. G. STUDIES ON THE MECHANISMS OF HYPERSENSITIVITY PHENOMENA. IX. HISTAMINE RELEASE FROM HUMAN LEUKOCYTES BY RAGWEED POLLEN ANTIGEN. J Exp Med. 1964 Oct 1;120:507–530. doi: 10.1084/jem.120.4.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lamm M. E., Boyse E. A., Old L. J., Lisowska-Bernstein B., Stockert E. Modulation of TL (thymus-leukemia) antigens by Fab-fragments of TL antibody. J Immunol. 1968 Jul;101(1):99–103. [PubMed] [Google Scholar]
  13. Magro A. M., Alexander A. In vitro studies of histamine release from rabbit leukocytes by divalent haptens. J Immunol. 1974 May;112(5):1757–1761. [PubMed] [Google Scholar]
  14. Röhlich P., Anderson P., Uvnäs B. Electron microscope observations on compounds 48-80-induced degranulation in rat mast cells. Evidence for sequential exocytosis of storage granules. J Cell Biol. 1971 Nov;51(21):465–483. doi: 10.1083/jcb.51.2.465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sullivan A. L., Grimley P. M., Metzger H. Electron microscopic localization of immunoglobulin E on the surface membrane of human basophils. J Exp Med. 1971 Dec 1;134(6):1403–1416. doi: 10.1084/jem.134.6.1403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. de Petris S., Raff M. C. Distribution of immunoglobulin on the surface of mouse lymphoid cells as determined by immunoferritin electron microscopy. Antibody-induced, temperature-dependent redistribution and its implications for membrane structure. Eur J Immunol. 1972 Dec;2(6):523–535. doi: 10.1002/eji.1830020611. [DOI] [PubMed] [Google Scholar]
  17. de Petris S., Raff M. C. Normal distribution, patching and capping of lymphocyte surface immunoglobulin studied by electron microscopy. Nat New Biol. 1973 Feb 28;241(113):257–259. doi: 10.1038/newbio241257a0. [DOI] [PubMed] [Google Scholar]

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