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. 1985 Apr;162(1):427–429. doi: 10.1128/jb.162.1.427-429.1985

Genetic locus (stmF) associated with cyclic GMP phosphodiesterase activity in Dictyostelium discoideum maps in linkage group II.

M B Coukell, A M Cameron
PMCID: PMC219006  PMID: 2984183

Abstract

Previous attempts to map the stmF locus in Dictyostelium discoideum, by using only clone morphology as a marker, have led to equivocal results. Since strains carrying mutations at the stmF locus possess very low cyclic GMP phosphodiesterase activity, we have remapped this locus using both morphological and biochemical markers. Our results indicate that mutations producing a stable "streamer" phenotype and reduced cyclic GMP phosphodiesterase activity are located in linkage group II, probably centromere distal to acrA.

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Selected References

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  1. Coukell M. B., Cameron A. M., Pitre C. M., Mee J. D. Developmental regulation and properties of the cGMP-specific phosphodiesterase in Dictyostelium discoideum. Dev Biol. 1984 May;103(1):246–257. doi: 10.1016/0012-1606(84)90026-5. [DOI] [PubMed] [Google Scholar]
  2. Coukell M. B., Roxby N. M. Linkage analysis of developmental mutations in aggregation-deficient mutants of Dictyostelium discoideum. Mol Gen Genet. 1977 Mar 16;151(3):275–288. doi: 10.1007/BF00268791. [DOI] [PubMed] [Google Scholar]
  3. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dicou E., Brachet P. A separate phosphodiesterase for the hydrolysis of cyclic guanosine 3',5'-monophosphate in growing Dictyostelium discoideum amoebae. Eur J Biochem. 1980 Aug;109(2):507–514. doi: 10.1111/j.1432-1033.1980.tb04822.x. [DOI] [PubMed] [Google Scholar]
  5. Free S. J., Schimke R. T. The structural gene for alpha-mannosidase-1 in Dictyostellium discoideum. Genetics. 1976 Oct;84(2):159–174. doi: 10.1093/genetics/84.2.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Katz E. R., Sussman M. Parasexual recombination in Dictyostelium discoideum: selection of stable diploid heterozygotes and stable haploid segregants (clones-temperature sensitive-ploidy-fruiting bodies-spore-slime mold). Proc Natl Acad Sci U S A. 1972 Feb;69(2):495–498. doi: 10.1073/pnas.69.2.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kessin R. H., Williams K. L., Newell P. C. Linkage analysis in Dictyostelium discoideum using temperature-sensitive growth mutants selected with bromodeoxyuridine. J Bacteriol. 1974 Sep;119(3):776–783. doi: 10.1128/jb.119.3.776-783.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lappano S., Coukell M. B. Evidence that intracellular cGMP is involved in regulating the extracellular cAMP phosphodiesterase and its specific inhibitor in Dictyostelium discoideum. Dev Biol. 1982 Sep;93(1):43–53. doi: 10.1016/0012-1606(82)90237-8. [DOI] [PubMed] [Google Scholar]
  10. Mato J. M., Krens F. A., van Haastert P. J., Konijn T. M. 3':5'-cyclic AMP-dependent 3':5'-cyclic GMP accumulation in Dictyostelium discoideum. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2348–2351. doi: 10.1073/pnas.74.6.2348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PONTECORVO G., KAFER E. Genetic analysis based on mitotic recombination. Adv Genet. 1958;9:71–104. [PubMed] [Google Scholar]
  12. Ratner D. I., Newell P. C. Linkage analysis in Dictyostelium discoideum using multiply marked tester strains: establishment of linkage group VII and the reassessment of earlier linkage data. J Gen Microbiol. 1978 Dec;109(2):225–236. doi: 10.1099/00221287-109-2-225. [DOI] [PubMed] [Google Scholar]
  13. Ross F. M., Newell P. C. Genetics of aggregation pattern mutations in the cellular slime mould Dictyostelium discoideum. J Gen Microbiol. 1979 Dec;115(2):289–300. doi: 10.1099/00221287-115-2-289. [DOI] [PubMed] [Google Scholar]
  14. Ross F. M., Newell P. C. Streamers: chemotactic mutants of Dictyostelium discoideum with altered cyclic GMP metabolism. J Gen Microbiol. 1981 Dec;127(2):339–350. doi: 10.1099/00221287-127-2-339. [DOI] [PubMed] [Google Scholar]
  15. Wallace J. S., Newell P. C. Genetic analysis by mitotic recombination in Dictyostelium discoideum of growth and developmental loci on linkage group VII. J Gen Microbiol. 1982 May;128(5):953–964. doi: 10.1099/00221287-128-5-953. [DOI] [PubMed] [Google Scholar]
  16. Ward A., Brenner M. Guanylate cyclase from Dictyostelium discoideum. Life Sci. 1977 Oct 1;21(7):997–1008. doi: 10.1016/0024-3205(77)90267-3. [DOI] [PubMed] [Google Scholar]
  17. Wurster B., Bumann J. Cell differentiation in the absence of intracellular cyclic AMP pulses in Dictyostelium discoideum. Dev Biol. 1981 Jul 15;85(1):262–265. doi: 10.1016/0012-1606(81)90257-8. [DOI] [PubMed] [Google Scholar]
  18. van Haastert P. J., van Lookeren Campagne M. M., Ross F. M. Altered cGMP-phosphodiesterase activity in chemotactic mutants of Dictyostelium discoideum. FEBS Lett. 1982 Oct 18;147(2):149–152. doi: 10.1016/0014-5793(82)81029-6. [DOI] [PubMed] [Google Scholar]

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