Abstract
The leukocytosis- and lymphocytosis-promoting factor (LPF) of Bordetella pertussis has been isolated to near homogeneity by physical, chemical, and electron microscopical criteria. LPF contains 14.5% nitrogen and is lipid and carbohydrate free. It is apparently composed of four polypeptide subunits. LPF caused leukocytosis and lymphocytosis in "nude" as well as in normal mice. In addition, purified LPF also induced histamine sensitization and hypoglycemia and refractoriness to the hyperglycemic effect of epinephrine. A monospecific LPF antiserum blocked these reactions as well as leukocytosis and lymphocytosis. LPF is clearly distinct from the hemagglutinating pili of B. pertussis.
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- Aprile M. A., Wardlaw A. C. Immunochemical studies on the lipopolysaccharides of Bordetella pertussis. Can J Microbiol. 1973 Feb;19(2):231–239. doi: 10.1139/m73-035. [DOI] [PubMed] [Google Scholar]
- CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
- DUGUID J. P. Fimbriae and adhesive properties in Klebsiella strains. J Gen Microbiol. 1959 Aug;21:271–286. doi: 10.1099/00221287-21-1-271. [DOI] [PubMed] [Google Scholar]
- FISHEL C. W., SZENTIVANYI A., TALMAGE D. W. Sensitization and desensitization of mice to histamine and serotonin by neurohumors. J Immunol. 1962 Jul;89:8–18. [PubMed] [Google Scholar]
- FISHEL C. W., SZENTIVANYI A. THE ABSENCE OF ADRENALINE-INDUCED HYPERGLYCEMIA IN PERTUSSIS- SENSITIZED MICE AND ITS RELATION TO HISTAMINE AND SEROTONIN HYPERSENSITIVITY. J Allergy. 1963 Sep-Oct;34:439–454. doi: 10.1016/0021-8707(63)90009-1. [DOI] [PubMed] [Google Scholar]
- Finger H., Mohr W., Hof H., Elekes E., Plager L. Einflubb von Bordetella pertussis auf das lymphatische Gewebe von Mäusen. 8. Der Einflubb von Bordetella pertussis auf das primäre und sekundäre Immunpotential von thymuslosen ("nude") Mäusen. Med Microbiol Immunol. 1973 Sep 26;159(1):13–38. doi: 10.1007/BF02122646. [DOI] [PubMed] [Google Scholar]
- KIND L. S. The altered reactivity of mice after inoculation with Bordetella pertussis vaccine. Bacteriol Rev. 1958 Sep;22(3):173–182. doi: 10.1128/br.22.3.173-182.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Keller K. F., Fishel C. W. In vivo and in vitro manifestations of adrenergic blockade in Bordetella pertussis-vaccinated mice. J Bacteriol. 1967 Oct;94(4):804–811. doi: 10.1128/jb.94.4.804-811.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lehrer S. B., Tan E. M., Vaughan J. H. Extraction and partial purification of the histamine-sensitizing factor of Bordetella pertussis. J Immunol. 1974 Jul;113(1):18–26. [PubMed] [Google Scholar]
- Lehrer S. B., Tan E. M., Vaughan J. H. Extraction and partial purification of the histamine-sensitizing factor of Bordetella pertussis. J Immunol. 1974 Jul;113(1):18–26. [PubMed] [Google Scholar]
- MACLENNAN A. P. Specific lipopolysaccharides of Bordetella. Biochem J. 1960 Feb;74:398–409. doi: 10.1042/bj0740398. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MASRY F. L. G. Production, extraction and purification of haemagglutinin of Haemophilus pertussis. J Gen Microbiol. 1952 Nov;7(3-4):201–210. doi: 10.1099/00221287-7-3-4-201. [DOI] [PubMed] [Google Scholar]
- MUNOZ J., HESTEKIN B. M. Method of preparing histamine sensitizing factor. Nature. 1962 Dec 22;196:1192–1193. doi: 10.1038/1961192a0. [DOI] [PubMed] [Google Scholar]
- Marsh J. B., Weinstein D. B. Simple charring method for determination of lipids. J Lipid Res. 1966 Jul;7(4):574–576. [PubMed] [Google Scholar]
- Morse J. H., Morse S. I. Studies on the ultrastructure of Bordetella pertussis. I. Morphology, origin, and biological activity of structures present in the extracellular fluid of liquid cultures of Bordetella pertussis. J Exp Med. 1970 Jun 1;131(6):1342–1357. doi: 10.1084/jem.131.6.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morse S. I., Bray K. K. The occurrence and properties of leukocytosis and lymphocytosis-stimulating material in the supernatant fluids of Bordetella pertussis cultures. J Exp Med. 1969 Mar 1;129(3):523–550. doi: 10.1084/jem.129.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Munoz J., Bergman R. K. Histamine-sensitizing factors from microbial agents, with special reference to Bordetella pertussis. Bacteriol Rev. 1968 Jun;32(2):103–126. doi: 10.1128/br.32.2.103-126.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakase Y., Tateishi M., Sekiya K., Kasuga T. Chemical and biological properties of the purified O antigen of Bordetella pertussis. Jpn J Microbiol. 1970 Jan;14(1):1–8. doi: 10.1111/j.1348-0421.1970.tb00485.x. [DOI] [PubMed] [Google Scholar]
- Ortez R. A., Seshachalam D., Szentivanyi A. Alterations in adenyl cyclase activity and glucose utilization of Bordetella pertussis-sensitized mouse spleen. Biochem Pharmacol. 1975 Jul 15;24(13-14):1297–1302. doi: 10.1016/0006-2952(75)90341-x. [DOI] [PubMed] [Google Scholar]
- Parker C. W., Morse S. I. The effect of Bordetella pertussis on lymphocyte cyclic AMP metabolism. J Exp Med. 1973 Apr 1;137(4):1078–1090. doi: 10.1084/jem.137.4.1078. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
- SZENTIVANYI A., FISHEL C. W., TALMAGE D. W. ADRENALINE MEDIATION OF HISTAMINE AND SEROTONIN HYPERGLYCEMIA IN NORMAL MICE AND THE ABSENCE OF ADRENALINE-INDUCED HYPERGLYCEMIA IN PERTUSSIS-SENSITIZED MICE. J Infect Dis. 1963 Sep-Oct;113:86–98. doi: 10.1093/infdis/113.2.86. [DOI] [PubMed] [Google Scholar]
- SZENTIVANYI A., FISHEL C. W., TALMAGE D. W. ADRENALINE MEDIATION OF HISTAMINE AND SEROTONIN HYPERGLYCEMIA IN NORMAL MICE AND THE ABSENCE OF ADRENALINE-INDUCED HYPERGLYCEMIA IN PERTUSSIS-SENSITIZED MICE. J Infect Dis. 1963 Sep-Oct;113:86–98. doi: 10.1093/infdis/113.2.86. [DOI] [PubMed] [Google Scholar]
- Sato Y., Arai H. Leucocytosis-promoting factor of Bordetella pertussis. I. Purification and characterization. Infect Immun. 1972 Dec;6(6):899–904. doi: 10.1128/iai.6.6.899-904.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sato Y., Arai H., Suzuki K. Leukocytosis-promoting factor of Bordetella pertussis. 3. Its identity with protective antigen. Infect Immun. 1974 May;9(5):801–810. doi: 10.1128/iai.9.5.801-810.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sato Y., Arai H., Suzuki K. Leukocytosis-promoting factor of Bordetella pertussis. II. Biological properties. Infect Immun. 1973 Jun;7(6):992–999. doi: 10.1128/iai.7.6.992-999.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tada T., Okumura K., Ochiai T., Iwasa S. Effect of lymphocytosis-promoting factor of Bordetella pertussis on the immune response. II. Adjuvant effect for the production of reaginic antibody in the rat. Int Arch Allergy Appl Immunol. 1972;43(2):207–216. doi: 10.1159/000230838. [DOI] [PubMed] [Google Scholar]
- Wortis H. H. Immunological responses of 'nude' mice. Clin Exp Immunol. 1971 Feb;8(2):305–317. [PMC free article] [PubMed] [Google Scholar]