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. 1985 Jul;163(1):262–266. doi: 10.1128/jb.163.1.262-266.1985

Chemotactic transducer proteins of Escherichia coli exhibit homology with methyl-accepting proteins from distantly related bacteria.

D M Nowlin, D O Nettleton, G W Ordal, G L Hazelbauer
PMCID: PMC219107  PMID: 3924893

Abstract

Transducers are transmembrane, methyl-accepting proteins central to the chemotactic systems of the enteric bacteria Escherichia coli and Salmonella typhimurium. Methyl-accepting proteins have been reported in a number of species in addition to these enteric bacteria. Those species include Bacillus subtilis and Spirochaeta aurantia, representatives of groups that diverged from ancestral enteric bacteria and from each other very early in bacterial evolution. An antiserum that reacts with all transducers of E. coli precipitated specifically methyl-accepting proteins from B. subtilis and S. aurantia, indicating that these proteins share antigenic determinants with transducers of E. coli. In addition, analysis of tryptic peptides by high-pressure liquid chromatography revealed similarities in the regions of methyl-accepting sites for proteins from all three species. These observations imply that structural features have been preserved in the three species from transducers contained in a common ancestor of eubacteria. It is thus reasonable to predict that other flagellated, chemotactic bacteria will be found to contain methyl-accepting proteins homologous to transducers of enteric bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahlgren J. A., Ordal G. W. Methyl esterification of glutamic acid residues of methyl-accepting chemotaxis proteins in Bacillus subtilis. Biochem J. 1983 Sep 1;213(3):759–763. doi: 10.1042/bj2130759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bollinger J., Park C., Harayama S., Hazelbauer G. L. Structure of the Trg protein: Homologies with and differences from other sensory transducers of Escherichia coli. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3287–3291. doi: 10.1073/pnas.81.11.3287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Boyd A., Kendall K., Simon M. I. Structure of the serine chemoreceptor in Escherichia coli. Nature. 1983 Feb 17;301(5901):623–626. doi: 10.1038/301623a0. [DOI] [PubMed] [Google Scholar]
  4. Boyd A., Simon M. Bacterial chemotaxis. Annu Rev Physiol. 1982;44:501–517. doi: 10.1146/annurev.ph.44.030182.002441. [DOI] [PubMed] [Google Scholar]
  5. Burgess-Cassler A., Ordal G. W. Functional homology of Bacillus subtilis methyltransferase II and Escherichia coli cheR protein. J Biol Chem. 1982 Nov 10;257(21):12835–12838. [PubMed] [Google Scholar]
  6. Canale-Parola E. Motility and chemotaxis of spirochetes. Annu Rev Microbiol. 1978;32:69–99. doi: 10.1146/annurev.mi.32.100178.000441. [DOI] [PubMed] [Google Scholar]
  7. Craven R. C., Montie T. C. Chemotaxis of Pseudomonas aeruginosa: involvement of methylation. J Bacteriol. 1983 May;154(2):780–786. doi: 10.1128/jb.154.2.780-786.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Engström P., Nowlin D., Bollinger J., Magnuson N., Hazelbauer G. L. Limited homology between trg and the other transducer proteins of Escherichia coli. J Bacteriol. 1983 Dec;156(3):1268–1274. doi: 10.1128/jb.156.3.1268-1274.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
  10. Goldman D. J., Worobec S. W., Siegel R. B., Hecker R. V., Ordal G. W. Chemotaxis in Bacillus subtilis: effects of attractants on the level of methylation of methyl-accepting chemotaxis proteins and the role of demethylation in the adaptation process. Biochemistry. 1982 Mar 2;21(5):915–920. doi: 10.1021/bi00534a016. [DOI] [PubMed] [Google Scholar]
  11. Gomes S. L., Shapiro L. Differential expression and positioning of chemotaxis methylation proteins in Caulobacter. J Mol Biol. 1984 Sep 25;178(3):551–568. doi: 10.1016/0022-2836(84)90238-9. [DOI] [PubMed] [Google Scholar]
  12. Hazelbauer G. L., Engström P., Harayama S. Methyl-accepting chemotaxis protein III and transducer gene trg. J Bacteriol. 1981 Jan;145(1):43–49. doi: 10.1128/jb.145.1.43-49.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hazelbauer G. L., Harayama S. Sensory transduction in bacterial chemotaxis. Int Rev Cytol. 1983;81:33–70. doi: 10.1016/s0074-7696(08)62334-7. [DOI] [PubMed] [Google Scholar]
  14. Hedblom M. L., Adler J. Genetic and biochemical properties of Escherichia coli mutants with defects in serine chemotaxis. J Bacteriol. 1980 Dec;144(3):1048–1060. doi: 10.1128/jb.144.3.1048-1060.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hirota N. Chemotaxis in thermophilic bacterium PS-3. J Biochem. 1984 Sep;96(3):645–650. doi: 10.1093/oxfordjournals.jbchem.a134880. [DOI] [PubMed] [Google Scholar]
  16. Kathariou S., Greenberg E. P. Chemoattractants elicit methylation of specific polypeptides in Spirochaeta aurantia. J Bacteriol. 1983 Oct;156(1):95–100. doi: 10.1128/jb.156.1.95-100.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kehry M. R., Dahlquist F. W. The methyl-accepting chemotaxis proteins of Escherichia coli. Identification of the multiple methylation sites on methyl-accepting chemotaxis protein I. J Biol Chem. 1982 Sep 10;257(17):10378–10386. [PubMed] [Google Scholar]
  18. Koiwai O., Hayashi H. Studies on bacterial chemotaxis. IV. Interaction of maltose receptor with a membrane-bound chemosensing component. J Biochem. 1979 Jul;86(1):27–34. [PubMed] [Google Scholar]
  19. Koshland D. E., Jr Biochemistry of sensing and adaptation in a simple bacterial system. Annu Rev Biochem. 1981;50:765–782. doi: 10.1146/annurev.bi.50.070181.004001. [DOI] [PubMed] [Google Scholar]
  20. Krikos A., Mutoh N., Boyd A., Simon M. I. Sensory transducers of E. coli are composed of discrete structural and functional domains. Cell. 1983 Jun;33(2):615–622. doi: 10.1016/0092-8674(83)90442-7. [DOI] [PubMed] [Google Scholar]
  21. Richarme G. Interaction of the maltose-binding protein with membrane vesicles of Escherichia coli. J Bacteriol. 1982 Feb;149(2):662–667. doi: 10.1128/jb.149.2.662-667.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Russo A. F., Koshland D. E., Jr Separation of signal transduction and adaptation functions of the aspartate receptor in bacterial sensing. Science. 1983 Jun 3;220(4601):1016–1020. doi: 10.1126/science.6302843. [DOI] [PubMed] [Google Scholar]
  23. Schimz A. Methylation of membrane proteins is involved in chemosensory and photosensory behavior of Halobacterium halobium. FEBS Lett. 1981 Mar 23;125(2):205–207. doi: 10.1016/0014-5793(81)80719-3. [DOI] [PubMed] [Google Scholar]
  24. Seymour F. W., Doetsch R. N. Chemotactic responses by motile bacteria. J Gen Microbiol. 1973 Oct;78(2):287–296. doi: 10.1099/00221287-78-2-287. [DOI] [PubMed] [Google Scholar]
  25. Shaw P., Gomes S. L., Sweeney K., Ely B., Shapiro L. Methylation involved in chemotaxis is regulated during Caulobacter differentiation. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5261–5265. [PMC free article] [PubMed] [Google Scholar]
  26. Springer M. S., Goy M. F., Adler J. Protein methylation in behavioural control mechanisms and in signal transduction. Nature. 1979 Jul 26;280(5720):279–284. doi: 10.1038/280279a0. [DOI] [PubMed] [Google Scholar]
  27. Ullah A. H., Ordal G. W. Purification and characterization of methyl-accepting chemotaxis protein methyltransferase I in Bacillus subtilis. Biochem J. 1981 Dec 1;199(3):795–805. doi: 10.1042/bj1990795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Wang E. A., Koshland D. E., Jr Receptor structure in the bacterial sensing system. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7157–7161. doi: 10.1073/pnas.77.12.7157. [DOI] [PMC free article] [PubMed] [Google Scholar]

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