Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Sep;163(3):850–857. doi: 10.1128/jb.163.3.850-857.1985

Nucleotide sequence of Klebsiella pneumoniae lac genes.

W E Buvinger, M Riley
PMCID: PMC219209  PMID: 3897196

Abstract

The nucleotide sequences of the Klebsiella pneumoniae lacI and lacZ genes and part of the lacY gene were determined, and these genes were located and oriented relative to one another. The K. pneumoniae lac operon is divergent in that the lacI and lacZ genes are oriented head to head, and complementary strands are transcribed. Besides base substitutions, the lacZ genes of K. pneumoniae and Escherichia coli have suffered short distance shifts of reading frame caused by additions or deletions or both during evolutionary divergence from a common ancestral gene. Relative to corresponding E. coli sequences, the nucleotide sequences of the lacZ and lacY genes are 61 and 67% conserved, and the lacI genes are 49% conserved. A comparison of both nucleotide and amino acid sequences revealed that the K. pneumoniae and E. coli lacI genes and lac repressor proteins each are related to the galR gene and gal repressor of E. coli to about the same extent. In terms of evolutionary relationships, the divergence of the forerunner of the galR gene from an ancestral lac repressor gene preceded separation and differentiation of the K. pneumoniae and E. coli lac repressor genes.

Full text

PDF
855

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUTTIN G. M'ECANISMES R'EGULATEURS DANS LA BIOSYNTH'ESE DES ENZYMES DU M'ETABOLISME DU GALACTOSE CHEZ ESCHERICHIA COLI K12. I. LA BIOSYNTH'ESE INDUITE DE LA GALACTOKINASE ET L'INDUCTION SIMULTAN'EE DE LA S'EQUENCE ENZYMATIQUE. J Mol Biol. 1963 Aug;7:164–182. doi: 10.1016/s0022-2836(63)80044-3. [DOI] [PubMed] [Google Scholar]
  2. Bocklage H., Müller-Hill B. lacZ--Y+ fusions in Escherichia coli. DNA sequencing reveals the eight N-terminal residues of lac permease as non-essential. Eur J Biochem. 1983 Dec 15;137(3):561–565. doi: 10.1111/j.1432-1033.1983.tb07862.x. [DOI] [PubMed] [Google Scholar]
  3. Brake A. J., Fowler A. V., Zabin I., Kania J., Müller-Hill B. beta-Galactosidase chimeras: primary structure of a lac repressor-beta-galactosidase protein. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4824–4827. doi: 10.1073/pnas.75.10.4824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buvinger W. E., Riley M. Regulatory region of the divergent Klebsiella pneumoniae lac operon. J Bacteriol. 1985 Sep;163(3):858–862. doi: 10.1128/jb.163.3.858-862.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Büchel D. E., Gronenborn B., Müller-Hill B. Sequence of the lactose permease gene. Nature. 1980 Feb 7;283(5747):541–545. doi: 10.1038/283541a0. [DOI] [PubMed] [Google Scholar]
  6. Conrad B., Mount D. W. Microcomputer programs for DNA sequence analysis. Nucleic Acids Res. 1982 Jan 11;10(1):31–38. doi: 10.1093/nar/10.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Farabaugh P. J. Sequence of the lacI gene. Nature. 1978 Aug 24;274(5673):765–769. doi: 10.1038/274765a0. [DOI] [PubMed] [Google Scholar]
  8. Grantham R., Gautier C., Gouy M., Jacobzone M., Mercier R. Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Res. 1981 Jan 10;9(1):r43–r74. doi: 10.1093/nar/9.1.213-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hong G. F. A systemic DNA sequencing strategy. J Mol Biol. 1982 Jul 5;158(3):539–549. doi: 10.1016/0022-2836(82)90213-3. [DOI] [PubMed] [Google Scholar]
  10. Kalnins A., Otto K., Rüther U., Müller-Hill B. Sequence of the lacZ gene of Escherichia coli. EMBO J. 1983;2(4):593–597. doi: 10.1002/j.1460-2075.1983.tb01468.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MacDonald C., Riley M. Cloning chromosomal lac genes of Klebsiella pneumoniae. Gene. 1983 Oct;24(2-3):341–345. doi: 10.1016/0378-1119(83)90096-3. [DOI] [PubMed] [Google Scholar]
  12. Messing J., Vieira J. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982 Oct;19(3):269–276. doi: 10.1016/0378-1119(82)90016-6. [DOI] [PubMed] [Google Scholar]
  13. Miller J. H., Coulondre C., Hofer M., Schmeissner U., Sommer H., Schmitz A., Lu P. Genetic studies of the lac repressor. IX. Generation of altered proteins by the suppression of nonsence mutations. J Mol Biol. 1979 Jun 25;131(2):191–222. doi: 10.1016/0022-2836(79)90073-1. [DOI] [PubMed] [Google Scholar]
  14. Miller J. H., Schmeissner U. Genetic studies of the lac repressor. X. Analysis of missense mutations in the lacI gene. J Mol Biol. 1979 Jun 25;131(2):223–248. doi: 10.1016/0022-2836(79)90074-3. [DOI] [PubMed] [Google Scholar]
  15. Musso R., Di Lauro R., Rosenberg M., de Crombrugghe B. Nucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Jan;74(1):106–110. doi: 10.1073/pnas.74.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Müller-Hill B., Kania J. Lac repressor can be fused to beta-galactosidase. Nature. 1974 Jun 7;249(457):561–563. doi: 10.1038/249561a0. [DOI] [PubMed] [Google Scholar]
  17. Nichols B. P., Blumenberg M., Yanofsky C. Comparison of the nucleoside sequence of trpA and sequences immediately beyond the trp operon of Klebsiella aerogenes. Salmonella typhimurium and Escherichia coli. Nucleic Acids Res. 1981 Apr 10;9(7):1743–1755. doi: 10.1093/nar/9.7.1743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pustell J., Kafatos F. C. A convenient and adaptable package of computer programs for DNA and protein sequence management, analysis and homology determination. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):643–655. doi: 10.1093/nar/12.1part2.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reeve E. C., Braithwaite J. A. The lactose system in Klebsiella aerogenes V9A. 4. A comparison of the lac operons of Klebsiella and Escherichia coli. Genet Res. 1974 Dec;24(3):323–331. doi: 10.1017/s0016672300015329. [DOI] [PubMed] [Google Scholar]
  20. Reeve E. C. The lactose system in Klebsiella aerogenes V9A. 3. Specific repression of the lac operon by melibiose and raffinose. Genet Res. 1973 Oct;22(2):217–221. doi: 10.1017/s0016672300013021. [DOI] [PubMed] [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yamagata H., Nakamura K., Inouye M. Comparison of the lipoprotein gene among the Enterobacteriaceae. DNA sequence of Erwinia amylovora lipoprotein gene. J Biol Chem. 1981 Mar 10;256(5):2194–2198. [PubMed] [Google Scholar]
  23. von Wilcken-Bergmann B., Müller-Hill B. Sequence of galR gene indicates a common evolutionary origin of lac and gal repressor in Escherichia coli. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2427–2431. doi: 10.1073/pnas.79.8.2427. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES