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. 1985 Sep;163(3):1288–1289. doi: 10.1128/jb.163.3.1288-1289.1985

supN ochre suppressor gene in Escherichia coli codes for tRNALys.

H Uemura, S Thorbjarnardóttir, V Gamulin, J Yano, O S Andrésson, D Söll, G Eggertsson
PMCID: PMC219277  PMID: 3897192

Abstract

We describe the cloning and nucleotide sequence of a new tRNALys gene, lysV, in Escherichia coli. An ochre suppressor allele of this gene, supN, codes for a tRNALys with anticodon UUA, presumably derived by a single base change from a wild-type UUU anticodon. The sequence of the supN tRNALys is identical to the sequence of ochre suppressor tRNAs encoded by mutant alleles at the lysT locus. This locus, which contains the two previously known tRNALys genes of E. coli, is located far from the lysV locus on the chromosome.

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Selected References

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  1. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 7. Microbiol Rev. 1983 Jun;47(2):180–230. doi: 10.1128/mr.47.2.180-230.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chakraburtty K., Steinschneider A., Case R. V., Mehler A. H. Primary structure of tRNA-Lys of E. coli B. Nucleic Acids Res. 1975 Nov;2(11):2069–2075. doi: 10.1093/nar/2.11.2069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eggertsson G., Adelberg E. A. Map positions and specificities of suppressor mutations in Escherichia coli K-12. Genetics. 1965 Aug;52(2):319–340. doi: 10.1093/genetics/52.2.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eggertsson G. Mapping of ochre suppressors in Escherichia coli. Genet Res. 1968 Feb;11(1):15–20. doi: 10.1017/s0016672300011150. [DOI] [PubMed] [Google Scholar]
  5. Gallucci E., Garen A. Suppressor genes for nonsense mutations. II. The su-4 and su-5 suppressor genes of Escherichia coli. J Mol Biol. 1966 Jan;15(1):193–200. doi: 10.1016/s0022-2836(66)80220-6. [DOI] [PubMed] [Google Scholar]
  6. Ikemura T., Ozeki H. Gross map location of Escherichia coli transfer RNA genes. J Mol Biol. 1977 Dec 5;117(2):419–446. doi: 10.1016/0022-2836(77)90136-x. [DOI] [PubMed] [Google Scholar]
  7. Messing J., Crea R., Seeburg P. H. A system for shotgun DNA sequencing. Nucleic Acids Res. 1981 Jan 24;9(2):309–321. doi: 10.1093/nar/9.2.309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Murgola E. J., Pagel F. T. Suppressors of lysine codons may be misacylated lysine tRNAs. J Bacteriol. 1983 Nov;156(2):917–919. doi: 10.1128/jb.156.2.917-919.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murray N. E., Brammar W. J., Murray K. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977 Jan 7;150(1):53–61. doi: 10.1007/BF02425325. [DOI] [PubMed] [Google Scholar]
  10. Prather N. E., Mims B. H., Murgola E. J. supG and supL in Escherichia coli code for mutant lysine tRNAs+. Nucleic Acids Res. 1983 Dec 10;11(23):8283–8286. doi: 10.1093/nar/11.23.8283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Prather N. E., Murgola E. J., Mims B. H. Nucleotide substitution in the amino acid acceptor stem of lysine transfer RNA causes missense suppression. J Mol Biol. 1984 Jan 15;172(2):177–184. doi: 10.1016/s0022-2836(84)80036-4. [DOI] [PubMed] [Google Scholar]
  12. Sakano H., Shimura Y. Characterization and in vitro processing of transfer RNA precursors accumulated in a temperature-sensitive mutant of Escherichia coli. J Mol Biol. 1978 Aug 15;123(3):287–326. doi: 10.1016/0022-2836(78)90082-7. [DOI] [PubMed] [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sullivan M. A., Cannon J. F., Webb F. H., Bock R. M. Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine. J Bacteriol. 1985 Jan;161(1):368–376. doi: 10.1128/jb.161.1.368-376.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thorbjarnardóttir S., Dingermann T., Rafnar T., Andrésson O. S., Söll D., Eggertsson G. Leucine tRNA family of Escherichia coli: nucleotide sequence of the supP(Am) suppressor gene. J Bacteriol. 1985 Jan;161(1):219–222. doi: 10.1128/jb.161.1.219-222.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yoshimura M., Kimura M., Ohno M., Inokuchi H., Ozeki H. Identification of transfer RNA suppressors in Escherichia coli. III. Ochre suppressors of lysine tRNA. J Mol Biol. 1984 Aug 25;177(4):609–625. doi: 10.1016/0022-2836(84)90040-8. [DOI] [PubMed] [Google Scholar]

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