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. 1982 Jul;151(1):135–143. doi: 10.1128/jb.151.1.135-143.1982

Replication terminus of the Bacillus subtilis chromosome.

M A O'Sullivan, C Anagnostopoulos
PMCID: PMC220215  PMID: 6282803

Abstract

Bidirectional replication of the Bacillus subtilis chromosome terminates at a point on the circular chromosome which is symmetrically opposite to the replication origin. Since replication rates are similar in both "halves" of the chromosome, termination presumably occurs at the meeting point of the two replication forks. To investigate whether the DNA sequence of this region of the chromosome contributes to the termination event, we have determined the latest replicating region of a chromosome in which this DNA sequence is no longer symmetrically opposite to the origin. The merodiploid strain GSY1127 has a very large nontandem duplication (approximately 25% of the total chromosome length) in the left-hand half of the chromosome, so that size and symmetry of this chromosome are grossly different from those of normal strains. We have examined the replication order of genetic markers in this strain by measuring subtilis terminal marker for replication remains a terminal marker in the merodiploid, i.e., replicates later than a marker situated symmetrically opposite to the replication origin. These results were supported by replication orders determined by pulse-density transfer experiments during synchronous replication. The data obtained indicate that there is a preferred site for the termination of replication in the B. subtilis chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams R. T., Wake R. G. Highly specific labeling of the Bacillus subtilis chromosome terminus. J Bacteriol. 1980 Aug;143(2):1036–1038. doi: 10.1128/jb.143.2.1036-1038.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anagnostopoulos C., Spizizen J. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS. J Bacteriol. 1961 May;81(5):741–746. doi: 10.1128/jb.81.5.741-746.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Audit C., Anagnostopoulos C. Genetic studies relating to the production of transformed clones diploid in the tryptophan region of the Bacillus subtilis genome. J Bacteriol. 1973 Apr;114(1):18–27. doi: 10.1128/jb.114.1.18-27.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Audit C., Anagnostopoulos C. Studies on the size of the diploid region in Bacillus subtilis merozygotes from strains carrying the trpE26 mutation. Mol Gen Genet. 1975;137(4):337–351. doi: 10.1007/BF00703259. [DOI] [PubMed] [Google Scholar]
  5. Gyurasits E. B., Wake R. G. Bidirectional chromosome replication in Bacillus subtilis. J Mol Biol. 1973 Jan;73(1):55–63. doi: 10.1016/0022-2836(73)90158-7. [DOI] [PubMed] [Google Scholar]
  6. Hara H., Yoshikawa H. Asymmetric bidirectional replication of Bacillus subtilis chromosome. Nat New Biol. 1973 Aug 15;244(137):200–203. doi: 10.1038/newbio244200a0. [DOI] [PubMed] [Google Scholar]
  7. Harford N. Bidirectional chromosome replication in Bacillus subtilis 168. J Bacteriol. 1975 Mar;121(3):835–847. doi: 10.1128/jb.121.3.835-847.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Henner D. J., Hoch J. A. The Bacillus subtilis chromosome. Microbiol Rev. 1980 Mar;44(1):57–82. doi: 10.1128/mr.44.1.57-82.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Horowitz S., Doyle R. J., Young F. E., Streips U. N. Selective association of the chromosome with membrane in a stable L-form of Bacillus subtilis. J Bacteriol. 1979 Jun;138(3):915–922. doi: 10.1128/jb.138.3.915-922.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ivarie R. D., Pène J. J. Association of the Bacillus subtilis chromosome with the cell membrane: resolution of free and bound deoxyribonucleic acid on renografin gradients. J Bacteriol. 1970 Nov;104(2):839–850. doi: 10.1128/jb.104.2.839-850.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuempel P. L., Duerr S. A., Seeley N. R. Terminus region of the chromosome in Escherichia coli inhibits replication forks. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3927–3931. doi: 10.1073/pnas.74.9.3927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lai C. J., Nathans D. Non-specific termination of simian virus 40 DNA replication. J Mol Biol. 1975 Sep 5;97(1):113–118. doi: 10.1016/s0022-2836(75)80026-x. [DOI] [PubMed] [Google Scholar]
  13. Lepesant-Kejzlarová J., Lepesant J. A., Walle J., Billault A., Dedonder R. Revision of the linkage map of Bacillus subtilis 168: indications for circularity of the chromosome. J Bacteriol. 1975 Mar;121(3):823–834. doi: 10.1128/jb.121.3.823-834.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Louarn J., Patte J., Louarn J. M. Evidence for a fixed termination site of chromosome replication in Escherichia coli K12. J Mol Biol. 1977 Sep 25;115(3):295–314. doi: 10.1016/0022-2836(77)90156-5. [DOI] [PubMed] [Google Scholar]
  15. Louarn J., Patte J., Louarn J. M. Map position of the replication terminus on the Escherichia coli chromosome. Mol Gen Genet. 1979 Apr 17;172(1):7–11. doi: 10.1007/BF00276208. [DOI] [PubMed] [Google Scholar]
  16. Matsushita T., White K. P., Sueoka N. Chromosom replication in toluenized Bacillus subtilis cells. Nat New Biol. 1971 Jul 28;232(30):111–114. doi: 10.1038/newbio232111a0. [DOI] [PubMed] [Google Scholar]
  17. Moses R. E., Richardson C. C. Replication and repair of DNA in cells of Escherichia coli treated with toluene. Proc Natl Acad Sci U S A. 1970 Oct;67(2):674–681. doi: 10.1073/pnas.67.2.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. O'Sullivan A., Howard K., Sueoka N. Location of a unique replication terminus and genetic evidence for partial bidirectional replication in the Bacillus subtilis chromosome. J Mol Biol. 1975 Jan 5;91(1):15–38. doi: 10.1016/0022-2836(75)90369-1. [DOI] [PubMed] [Google Scholar]
  19. O'Sullivan A., Sueoka N. Sequential replication of the Bacillus subtilis chromosome. IV. Genetic mapping by density transfer experiment. J Mol Biol. 1967 Jul 28;27(2):349–368. doi: 10.1016/0022-2836(67)90025-3. [DOI] [PubMed] [Google Scholar]
  20. O'Sullivan M. A., Howard K., Sueoka N. On the bidirectional replication of the Bacillus subtilis chromosome. J Mol Biol. 1975 Dec 5;99(2):347–348. doi: 10.1016/s0022-2836(75)80151-3. [DOI] [PubMed] [Google Scholar]
  21. Sargent M. G. Specific labeling of the Bacillus subtilis chromosome terminus. J Bacteriol. 1980 Aug;143(2):1033–1035. doi: 10.1128/jb.143.2.1033-1035.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Snyder R. W., Young F. E. Association between the chromosome and the cytoplasmic membrane in Bacillus subtilis. Biochem Biophys Res Commun. 1969 May 8;35(3):354–362. doi: 10.1016/0006-291x(69)90506-3. [DOI] [PubMed] [Google Scholar]
  23. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sueoka N., Hammers J. M. Isolation of DNA-membrane complex in Bacillus subtilis. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4787–4791. doi: 10.1073/pnas.71.12.4787. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sueoka N., Quinn W. G. Membrane attachment of the chromosome replication origin in Bacillus subtilis. Cold Spring Harb Symp Quant Biol. 1968;33:695–705. doi: 10.1101/sqb.1968.033.01.078. [DOI] [PubMed] [Google Scholar]
  26. Sueoka N., Yoshikawa H. The chromosome of Bacillus subtilis. I. Theory of marker frequency analysis. Genetics. 1965 Oct;52(4):747–757. doi: 10.1093/genetics/52.4.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Trowsdale J., Anagnostopoulos C. Evidence for the Translocation of a Chromosome Sement in Bacillus subtilis Strains Carrying the trpE26 Mutation. J Bacteriol. 1975 Jun;122(3):886–898. doi: 10.1128/jb.122.3.886-898.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Valenzuela M. S., Freifelder D. Lack of a unique termination site for the first round of bacteriophage lambda DNA replication. J Mol Biol. 1976 Apr 15;102(3):569–589. doi: 10.1016/0022-2836(76)90335-1. [DOI] [PubMed] [Google Scholar]

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