Abstract
The determinant of resistance to fosfomycin of the Serratia marcescens plasmid pOU900 was transposed into the plasmid ColE1 and into the plasmid RP4 in the absence of the RecA function of the host. In each case, the acquisition of fosfomycin resistance was correlated with the presence of a discrete piece of DNA, uniform in size and in restriction pattern, This new, 7.5-megadalton transposable element encoding resistance to fosfomycin has been called Tn2921. A preliminary map of the transposon is presented.
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- Arthur A., Sherratt D. Dissection of the transposition process: a transposon-encoded site-specific recombination system. Mol Gen Genet. 1979 Oct 1;175(3):267–274. doi: 10.1007/BF00397226. [DOI] [PubMed] [Google Scholar]
- Campbell A., Starlinger P., Berg D. E., Botstein D., Lederberg E. M., Novick R. P., Szybalski W. Nomenclature of transposable elements in prokaryotes. Plasmid. 1979 Jul;2(3):466–473. doi: 10.1016/0147-619x(79)90030-1. [DOI] [PubMed] [Google Scholar]
- Heffron F., Bedinger P., Champoux J. J., Falkow S. Deletions affecting the transposition of an antibiotic resistance gene. Proc Natl Acad Sci U S A. 1977 Feb;74(2):702–706. doi: 10.1073/pnas.74.2.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kahan F. M., Kahan J. S., Cassidy P. J., Kropp H. The mechanism of action of fosfomycin (phosphonomycin). Ann N Y Acad Sci. 1974 May 10;235(0):364–386. doi: 10.1111/j.1749-6632.1974.tb43277.x. [DOI] [PubMed] [Google Scholar]
- Mendoza C., Garcia J. M., Llaneza J., Mendez F. J., Hardisson C., Ortiz J. M. Plasmid-determined resistance to fosfomycin in Serratia marcescens. Antimicrob Agents Chemother. 1980 Aug;18(2):215–219. doi: 10.1128/aac.18.2.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rubens C. E., McNeill W. F., Farrar W. E., Jr Transposable plasmid deoxyribonucleic acid sequence in Pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents. J Bacteriol. 1979 Sep;139(3):877–882. doi: 10.1128/jb.139.3.877-882.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsuruoka T., Yamada Y. Charactertization of spontaneous fosfomycin (phosphonomycin)-resistant cells of Escherichia coli B in vitro. J Antibiot (Tokyo) 1975 Nov;28(11):906–911. doi: 10.7164/antibiotics.28.906. [DOI] [PubMed] [Google Scholar]