Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1982 Jul;151(1):491–494. doi: 10.1128/jb.151.1.491-494.1982

Synthesis of penicillin-binding protein 6 by stationary-phase Escherichia coli.

C E Buchanan, M O Sowell
PMCID: PMC220269  PMID: 7045084

Abstract

The level of penicillin-binding protein 6, a D-alanine carboxypeptidase I, was found to be 2- to 10-fold higher in stationary-phase cells than in exponentially growing cells of Escherichia coli. This increase appeared to be due to de novo synthesis rather than to an unmasking of preexisting material. There was no comparable change in the amount of any of the other six penicillin-binding proteins.

Full text

PDF
493

Images in this article

492Image
on p.492

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldea M., Herrero E., Esteve M. I., Guerrero R. Surface density of major outer membrane proteins in Salmonella typhimurium in different growth conditions. J Gen Microbiol. 1980 Oct;120(2):355–367. doi: 10.1099/00221287-120-2-355. [DOI] [PubMed] [Google Scholar]
  2. Amanuma H., Strominger J. L. Purification and properties of penicillin-binding proteins 5 and 6 from Escherichia coli membranes. J Biol Chem. 1980 Dec 10;255(23):11173–11180. [PubMed] [Google Scholar]
  3. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  4. Buchanan C. E. Topographical distribution of penicillin-binding proteins in the Escherichia coli membrane. J Bacteriol. 1981 Mar;145(3):1293–1298. doi: 10.1128/jb.145.3.1293-1298.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fontana R., Canepari P., Satta G., Coyette J. Identification of the lethal target of benzylpenicillin in Streptococcus faecalis by in vivo penicillin binding studies. Nature. 1980 Sep 4;287(5777):70–72. doi: 10.1038/287070a0. [DOI] [PubMed] [Google Scholar]
  6. Frazer A. C., Curtiss R., 3rd Production, properties and utility of bacterial minicells. Curr Top Microbiol Immunol. 1975;69:1–84. doi: 10.1007/978-3-642-50112-8_1. [DOI] [PubMed] [Google Scholar]
  7. Goldberg A. L., St John A. C. Intracellular protein degradation in mammalian and bacterial cells: Part 2. Annu Rev Biochem. 1976;45:747–803. doi: 10.1146/annurev.bi.45.070176.003531. [DOI] [PubMed] [Google Scholar]
  8. Harkness R. E., Mirelman D., Ishiguro E. E. Regulation of D-alanine carboxypeptidase and peptidoglycan cross-linkage in amino acid-deprived Escherichia coli. J Bacteriol. 1981 Feb;145(2):845–849. doi: 10.1128/jb.145.2.845-849.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Inouye M., Pardee A. B. Changes of membrane proteins and their relation to deoxyribonucleic acid synthesis and cell division of Escherichia coli. J Biol Chem. 1970 Nov 10;245(21):5813–5819. [PubMed] [Google Scholar]
  10. Ishiguro E. E., Mirelman D., Harkness R. E. Regulation of the terminal steps in peptidoglycan biosynthesis in ether-treated cells of Escherichia coli. FEBS Lett. 1980 Nov 3;120(2):175–178. doi: 10.1016/0014-5793(80)80291-2. [DOI] [PubMed] [Google Scholar]
  11. Ishiguro E. E., Ramey W. D. Stringent control of peptidoglycan biosynthesis in Escherichia coli K-12. J Bacteriol. 1976 Sep;127(3):1119–1126. doi: 10.1128/jb.127.3.1119-1126.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Iwaya M., Strominger J. L. Simultaneous deletion of D-alanine carboxypeptidase IB-C and penicillin-binding component IV in a mutant of Escherichia coli K12. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2980–2984. doi: 10.1073/pnas.74.7.2980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kuriki Y. Effects of unsaturated fatty acids on the morphogenesis of an unsaturated fatty acid auxotroph of Escherichia coli. J Bacteriol. 1981 Sep;147(3):1121–1124. doi: 10.1128/jb.147.3.1121-1124.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lugtenberg B., Peters R., Bernheimer H., Berendsen W. Influence of cultural conditions and mutations on the composition of the outer membrane proteins of Escherichia coli. Mol Gen Genet. 1976 Sep 23;147(3):251–262. doi: 10.1007/BF00582876. [DOI] [PubMed] [Google Scholar]
  15. MANDELSTAM J. Turnover of protein in growing and non-growing populations of Escherichia coli. Biochem J. 1958 May;69(1):110–119. doi: 10.1042/bj0690110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Matsuhashi M., Takagaki Y., Maruyama I. N., Tamaki S., Nishimura Y., Suzuki H., Ogino U., Hirota Y. Mutants of Escherichia coli lacking in highly penicillin-sensitive D-alanine carboxypeptidase activity. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2976–2979. doi: 10.1073/pnas.74.7.2976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Matsuhashi M., Tamaki S., Curtis S. J., Strominger J. L. Mutational evidence for identity of penicillin-binding protein 5 in Escherichia coli with the major D-alanine carboxypeptidase IA activity. J Bacteriol. 1979 Jan;137(1):644–647. doi: 10.1128/jb.137.1.644-647.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mirelman D., Yashouv-Gan Y., Schwarz U. Peptidoglycan biosynthesis in a thermosensitive division mutant of Escherichia coli. Biochemistry. 1976 May 4;15(9):1781–1790. doi: 10.1021/bi00654a001. [DOI] [PubMed] [Google Scholar]
  19. Pratt J. M., Holland I. B., Spratt B. G. Precursor forms of penicillin-binding proteins 5 and 6 of E. coli cytoplasmic membrane. Nature. 1981 Sep 24;293(5830):307–309. doi: 10.1038/293307a0. [DOI] [PubMed] [Google Scholar]
  20. Spratt B. G. Biochemical and genetical approaches to the mechanism of action of penicillin. Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):273–283. doi: 10.1098/rstb.1980.0045. [DOI] [PubMed] [Google Scholar]
  21. Spratt B. G. Properties of the penicillin-binding proteins of Escherichia coli K12,. Eur J Biochem. 1977 Jan;72(2):341–352. doi: 10.1111/j.1432-1033.1977.tb11258.x. [DOI] [PubMed] [Google Scholar]
  22. Suzuki H., Nishimura Y., Hirota Y. On the process of cellular division in Escherichia coli: a series of mutants of E. coli altered in the penicillin-binding proteins. Proc Natl Acad Sci U S A. 1978 Feb;75(2):664–668. doi: 10.1073/pnas.75.2.664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vosberg H. P., Hoffmann-Berling H. DNA synthesis in nucleotide-permeable Escherichia coli cells. I. Preparation and properties of ether-treated cells. J Mol Biol. 1971 Jun 28;58(3):739–753. doi: 10.1016/0022-2836(71)90037-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES