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. 1982 Aug;151(2):1027–1034. doi: 10.1128/jb.151.2.1027-1034.1982

Manganese binding and oxidation by spores of a marine bacillus.

R A Rosson, K H Nealson
PMCID: PMC220357  PMID: 6212577

Abstract

Mature, dormant spores of a marine bacillus, SG-1, bound and oxidized (precipitated) manganese on their surfaces. The binding and oxidation occurred under dormant conditions, with mature spores suspended in natural seawater. These heat-stable spores were formed in the absence of added manganese in the growth medium. The rate and amount of manganese bound by SG-1 spores was a function of spore concentration. Temperatures greater than 45 degrees C, pH values below 6.5, or the addition of EDTA or the metabolic inhibitors sodium azide, potassium cyanide, and mercuric chloride inhibited manganese binding and oxidation. However, SG-1 spores bound and oxidized manganese after treatment with glutaraldehyde, formaldehyde, ethylene oxide gas, or UV light, all of which killed the spores. Manganese oxidation never occurred in the absence of manganese binding to spores. The data suggest that Mn2+ was complexed by a spore component, perhaps an exosporium or a spore coat protein: once bound, the manganese was rapidly oxidized.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki H., Slepecky R. A. Inducement of a heat-shock requirement for germination and production of increased heat resistance in Bacillus fastidiosus spores by manganous ions. J Bacteriol. 1973 Apr;114(1):137–143. doi: 10.1128/jb.114.1.137-143.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHARNEY J., FISHER W. P., HEGARTY C. P. Managanese as an essential element for sporulation in the genus Bacillus. J Bacteriol. 1951 Aug;62(2):145–148. doi: 10.1128/jb.62.2.145-148.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ehrlich H. L. Bacteriology of Manganese Nodules: I. Bacterial Action on Manganese in Nodule Enrichments. Appl Microbiol. 1963 Jan;11(1):15–19. doi: 10.1128/am.11.1.15-19.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eisenstadt E., Fisher S., Der C. L., Silver S. Manganese transport in Bacillus subtilis W23 during growth and sporulation. J Bacteriol. 1973 Mar;113(3):1363–1372. doi: 10.1128/jb.113.3.1363-1372.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GOULD G. W., HITCHINS A. D. SENSITIZATION OF BACTERIAL SPORES TO LYSOZYME AND TO HYDROGEN PEROXIDE WITH AGENTS WHICH RUPTURE DISULPHIDE BONDS. J Gen Microbiol. 1963 Dec;33:413–423. doi: 10.1099/00221287-33-3-413. [DOI] [PubMed] [Google Scholar]
  6. Goldman R. C., Tipper D. J. Bacillus subtilis spore coats: complexity and purification of a unique polypeptide component. J Bacteriol. 1978 Sep;135(3):1091–1106. doi: 10.1128/jb.135.3.1091-1106.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hajj H., Makemson J. Determination of growth of Sphaerotilus discophorus in the presence of manganese. Appl Environ Microbiol. 1976 Nov;32(5):699–702. doi: 10.1128/aem.32.5.699-702.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jung W. K., Schweisfurth R. Manganese oxidation by an intracellular protein of a Pseudomonas species. Z Allg Mikrobiol. 1979;19(2):107–115. [PubMed] [Google Scholar]
  9. Kepkay P. E., Nealson K. H. Surface enhancement of sporulation and manganese oxidation by a marine bacillus. J Bacteriol. 1982 Aug;151(2):1022–1026. doi: 10.1128/jb.151.2.1022-1026.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kondo M., Foster J. W. Chemical and electron microscope studies on fractions prepared from coats of Bacillus spores. J Gen Microbiol. 1967 May;47(2):257–271. doi: 10.1099/00221287-47-2-257. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Schulz-Baldes M., Lewin R. A. Manganese encrustation of zygospores of a chlamydomonas (chlorophyta: volvocales). Science. 1975 Jun 13;188(4193):1119–1120. doi: 10.1126/science.188.4193.1119. [DOI] [PubMed] [Google Scholar]
  13. van Veen W. L., Mulder E. G., Deinema M. H. The Sphaerotilus-Leptothrix group of bacteria. Microbiol Rev. 1978 Jun;42(2):329–356. doi: 10.1128/mr.42.2.329-356.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. van Veen W. L. Proceedings: Netherlands Society for Microbiology meeting at Utrecht on 2 May 1973. Biological xoidation of manganese in soils. Antonie Van Leeuwenhoek. 1973 Nov;39(4):657–662. doi: 10.1007/BF02578915. [DOI] [PubMed] [Google Scholar]

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