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. 1982 Sep;151(3):1129–1135. doi: 10.1128/jb.151.3.1129-1135.1982

Expression of a copy number control gene (copB) of plasmid R1 is constitutive and growth rate dependent.

J Light, S Molin
PMCID: PMC220388  PMID: 7050077

Abstract

The copy number control gene copB from plasmid R1 was fused to the lacZ gene in vitro, resulting in expression of a fused polypeptide consisting of the first 53 amino acids of the CopB polypeptide and the beta-galactosidase polypeptide minus its first 8 amino acids. Based on measurements of specific activities of this fused protein under various conditions, it was concluded that expression of copB is gene dosage dependent, unregulated by plasmid-coded functions, and proportional to growth rate between 0.4 and 2.0 doublings per h. The rate of expression of the copB gene is surprisingly high compared with other known cases of regulatory proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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