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. 1982 Oct;152(1):506–509. doi: 10.1128/jb.152.1.506-509.1982

Sequence analysis of the heat-labile enterotoxin subunit B gene originating in human enterotoxigenic Escherichia coli.

T Yamamoto, T Tamura, M Ryoji, A Kaji, T Yokota, T Takano
PMCID: PMC221452  PMID: 6749816

Abstract

In this study, we determined the amino-terminal coding sequence, covering the signal peptide and the amino-terminus of the mature peptide, of the heat-labile enterotoxin subunit B (LT-B) gene originating in human enterotoxigenic Escherichia coli. Neither the signal sequence nor the amino-terminal sequence of the mature LT-B was identical to those sequences from porcine enterotoxigenic E. coli, but there was an extensive homology.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clements J. D., Yancey R. J., Finkelstein R. A. Properties of homogeneous heat-labile enterotoxin from Escherichia coli. Infect Immun. 1980 Jul;29(1):91–97. doi: 10.1128/iai.29.1.91-97.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dallas W. S., Falkow S. Amino acid sequence homology between cholera toxin and Escherichia coli heat-labile toxin. Nature. 1980 Dec 4;288(5790):499–501. doi: 10.1038/288499a0. [DOI] [PubMed] [Google Scholar]
  3. Dallas W. S., Gill D. M., Falkow S. Cistrons encoding Escherichia coli heat-labile toxin. J Bacteriol. 1979 Sep;139(3):850–858. doi: 10.1128/jb.139.3.850-858.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gill D. M., Clements J. D., Robertson D. C., Finkelstein R. A. Subunit number and arrangement in Escherichia coli heat-labile enterotoxin. Infect Immun. 1981 Sep;33(3):677–682. doi: 10.1128/iai.33.3.677-682.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gill D. M., Richardson S. H. Adenosine diphosphate-ribosylation of adenylate cyclase catalyzed by heat-labile enterotoxin of Escherichia coli: comparison with cholera toxin. J Infect Dis. 1980 Jan;141(1):64–70. doi: 10.1093/infdis/141.1.64. [DOI] [PubMed] [Google Scholar]
  6. Honda T., Tsuji T., Takeda Y., Miwatani T. Immunological nonidentity of heat-labile enterotoxins from human and porcine enterotoxigenic Escherichia coli. Infect Immun. 1981 Nov;34(2):337–340. doi: 10.1128/iai.34.2.337-340.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Klapper D. G., Finkelstein R. A., Capra J. D. Subunit structure and N-terminal amino acid sequence of the three chains of cholera enterotoxin. Immunochemistry. 1976 Jul;13(7):605–611. doi: 10.1016/0019-2791(76)90173-7. [DOI] [PubMed] [Google Scholar]
  8. Kurosky A., Markel D. E., Peterson J. W., Fitch W. M. Primary structure of cholera toxin beta-chain: a glycoprotein hormone analog? Science. 1977 Jan 21;195(4275):299–301. doi: 10.1126/science.831277. [DOI] [PubMed] [Google Scholar]
  9. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  10. Palva E. T., Hirst T. R., Hardy S. J., Holmgren J., Randall L. Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli. J Bacteriol. 1981 Apr;146(1):325–330. doi: 10.1128/jb.146.1.325-330.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ryoji M., Berland R., Kaji A. Reinitiation of translation from the triplet next to the amber termination codon in the absence of ribosome-releasing factor. Proc Natl Acad Sci U S A. 1981 Oct;78(10):5973–5977. doi: 10.1073/pnas.78.10.5973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith H. W., Gyles C. L. The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin. J Med Microbiol. 1970 Aug;3(3):387–401. doi: 10.1099/00222615-3-3-387. [DOI] [PubMed] [Google Scholar]
  13. Spicer E. K., Kavanaugh W. M., Dallas W. S., Falkow S., Konigsberg W. H., Schafer D. E. Sequence homologies between A subunits of Escherichia coli and Vibrio cholerae enterotoxins. Proc Natl Acad Sci U S A. 1981 Jan;78(1):50–54. doi: 10.1073/pnas.78.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Takeda Y., Honda T., Taga S., Miwatani T. In vitro formation of hybrid toxins between subunits of Escherichia coli heat-labile enterotoxin and those of cholera enterotoxin. Infect Immun. 1981 Nov;34(2):341–346. doi: 10.1128/iai.34.2.341-346.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tamura T., Noda M., Takano T. Structure of the baboon endogenous virus genome: nucleotide sequences of the long terminal repeat. Nucleic Acids Res. 1981 Dec 11;9(23):6615–6626. doi: 10.1093/nar/9.23.6615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yamamoto T., Yokota T. Escherichia coli heat-labile enterotoxin genes are flanked by repeated deoxyribonucleic acid sequences. J Bacteriol. 1981 Feb;145(2):850–860. doi: 10.1128/jb.145.2.850-860.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yamamoto T., Yokota T., Kaji A. Molecular organization of heat-labile enterotoxin genes originating in Escherichia coli of human origin and construction of heat-labile toxoid-producing strains. J Bacteriol. 1981 Dec;148(3):983–987. doi: 10.1128/jb.148.3.983-987.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yamamoto T., Yokota T. Release of heat-labile enterotoxin subunits by Escherichia coli. J Bacteriol. 1982 Jun;150(3):1482–1484. doi: 10.1128/jb.150.3.1482-1484.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. von Heijne G. Trans-membrane translocation of proteins. A detailed physico-chemical analysis. Eur J Biochem. 1980 Feb;103(3):431–438. doi: 10.1111/j.1432-1033.1980.tb05966.x. [DOI] [PubMed] [Google Scholar]

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