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. 1977 Jan 1;69(1):97–120. doi: 10.1085/jgp.69.1.97

Rhodopsin photoproducts and rod sensitivity in the skate retina

PMCID: PMC2215046  PMID: 833567

Abstract

The late photoproducts that result from the isomerization of rhodopsin have been identified in the isolated all-rod retina of the skate by means of rapid spectrophotometry. The sequence in which these intermediates form and decay could be described by a scheme that incorporates two pathways for the degradation of metarhodopsin II (MII) to retinol: one via metarhodopsin III (MIII) and the other (which bypasses MIII) through retinal. Computer simulation of the model yielded rate constants and spectral absorbance coefficients for the late photoproducts which fit experimental data obtained at temperatures ranging from 7 degrees C to 27 degrees C. Comparing the kinetics of the thermal reactions with the changes in rod threshold that occur during dark adaptation indicated that the decay of MII and the fall in receptor thresholds exhibit similarities with regard to their temperature dependence. However, the addition of 2 mM hydroxylamine to a perfusate bathing the retina greatly accelerated the photochemical reactions, but had no significant effect on the rate of recovery of rod sensitivity. It appears, therefore, that the late bleaching intermediates do not control the sensitivities of skate rods during dark adaptation.

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Selected References

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  1. Abrahamson E. W., Ostroy S. E. The photochemical and macromolecular aspects of vision. Prog Biophys Mol Biol. 1967;17:179–215. doi: 10.1016/0079-6107(67)90007-7. [DOI] [PubMed] [Google Scholar]
  2. Baumann C. Kinetics of slow thermal reactions during the bleaching of rhodopsin in the perfused frog retina. J Physiol. 1972 May;222(3):643–663. doi: 10.1113/jphysiol.1972.sp009819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cone R. A., Cobbs W. H., 3rd Rhodopsin cycle in the living eye of the rat. Nature. 1969 Mar 1;221(5183):820–822. doi: 10.1038/221820a0. [DOI] [PubMed] [Google Scholar]
  4. Cone R. A. Rotational diffusion of rhodopsin in the visual receptor membrane. Nat New Biol. 1972 Mar 15;236(63):39–43. doi: 10.1038/newbio236039a0. [DOI] [PubMed] [Google Scholar]
  5. DARTNALL H. J. A. The interpretation of spectral sensitivity curves. Br Med Bull. 1953;9(1):24–30. doi: 10.1093/oxfordjournals.bmb.a074302. [DOI] [PubMed] [Google Scholar]
  6. DOWLING J. E. NEURAL AND PHOTOCHEMICAL MECHANISMS OF VISUAL ADAPTATION IN THE RAT. J Gen Physiol. 1963 Jul;46:1287–1301. doi: 10.1085/jgp.46.6.1287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Donner K. O., Reuter T. Dark-adaptation processes in the rhodopsin rods of the frog's retina. Vision Res. 1967 Jan;7(1):17–41. doi: 10.1016/0042-6989(67)90023-5. [DOI] [PubMed] [Google Scholar]
  8. Donner K. O., Reuter T. Visual adaptation of the rhodopsin rods in the frogs retina. J Physiol. 1968 Nov;199(1):59–87. doi: 10.1113/jphysiol.1968.sp008639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dowling J. E., Ripps H. Adaptation in skate photoreceptors. J Gen Physiol. 1972 Dec;60(6):698–719. doi: 10.1085/jgp.60.6.698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dowling J. E., Ripps H. S-potentials in the skate retina. Intracellular recordings during light and dark adaptation. J Gen Physiol. 1971 Aug;58(2):163–189. doi: 10.1085/jgp.58.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dowling J. E., Ripps H. Visual adaptation in the retina of the skate. J Gen Physiol. 1970 Oct;56(4):491–520. doi: 10.1085/jgp.56.4.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ebrey T. G. The thermal decay of the intermediates of rhodopsin in situ. Vision Res. 1968 Aug;8(8):965–982. doi: 10.1016/0042-6989(68)90071-0. [DOI] [PubMed] [Google Scholar]
  13. FURUKAWA T., HANAWA I. Effects of some common cations on electroretinogram of the toad. Jpn J Physiol. 1955 Dec 15;5(4):289–300. doi: 10.2170/jjphysiol.5.289. [DOI] [PubMed] [Google Scholar]
  14. FUTTERMAN S. Metabolism of the retina. III. The role of reduced triphoshopyridine nucleotide in the visual cycle. J Biol Chem. 1963 Mar;238:1145–1150. [PubMed] [Google Scholar]
  15. Frank R. N., Dowling J. E. Rhodopsin photoproducts: effects on electroretinogram sensitivity in isolated perfused rat retina. Science. 1968 Aug 2;161(3840):487–489. doi: 10.1126/science.161.3840.487. [DOI] [PubMed] [Google Scholar]
  16. Frank R. N. Properties of "neural" adaptation in components of the frog electroretinogram. Vision Res. 1971 Oct;11(10):1113–1123. doi: 10.1016/0042-6989(71)90115-5. [DOI] [PubMed] [Google Scholar]
  17. MATTHEWS R. G., HUBBARD R., BROWN P. K., WALD G. TAUTOMERIC FORMS OF METARHODOPSIN. J Gen Physiol. 1963 Nov;47:215–240. doi: 10.1085/jgp.47.2.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NOELL W. K. The origin of the electroretinogram. Am J Ophthalmol. 1954 Jul;38(12):78–90. doi: 10.1016/0002-9394(54)90012-4. [DOI] [PubMed] [Google Scholar]
  19. Ostroy S. E., Erhardt F., Abrahamson E. W. Protein configuration changes in the photolysis of rhodopsin. II. The sequence of intermediates in thermal decay of cattle metarhodopsin in vitro. Biochim Biophys Acta. 1966 Feb 7;112(2):265–277. doi: 10.1016/0926-6585(66)90326-8. [DOI] [PubMed] [Google Scholar]
  20. Penn R. D., Hagins W. A. Signal transmission along retinal rods and the origin of the electroretinographic a-wave. Nature. 1969 Jul 12;223(5202):201–204. doi: 10.1038/223201a0. [DOI] [PubMed] [Google Scholar]
  21. Ripps H., Snapper A. G. Computer analysis of photochemical changes in the human retina. Comput Biol Med. 1974 Jun;4(1):107–122. doi: 10.1016/0010-4825(74)90010-9. [DOI] [PubMed] [Google Scholar]
  22. Ripps H., Weale R. A. Rhodopsin regeneration in man. Nature. 1969 May 24;222(5195):775–777. doi: 10.1038/222775a0. [DOI] [PubMed] [Google Scholar]
  23. SICKEL W. RESPIRATORY AND ELECTRICAL RESPONSES TO LIGHT SIMULATION IN THE RETINA OF THE FROG. Science. 1965 Apr 30;148(3670):648–651. doi: 10.1126/science.148.3670.648. [DOI] [PubMed] [Google Scholar]
  24. Sillman A. J., Ito H., Tomita T. Studies on the mass receptor potential of the isolated frog retina. I. General properties of the response. Vision Res. 1969 Dec;9(12):1435–1442. doi: 10.1016/0042-6989(69)90059-5. [DOI] [PubMed] [Google Scholar]
  25. WALD G., BROWN P. K. The molar extinction of rhodopsin. J Gen Physiol. 1953 Nov 20;37(2):189–200. doi: 10.1085/jgp.37.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. WALD G., HUBBARD R. The reduction of retinene1 to vitamina A1 in vitro. J Gen Physiol. 1949 Jan;32(3):367–389. doi: 10.1085/jgp.32.3.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. WEALE R. A. Observations on photo-chemical reactions in living eyes. Br J Ophthalmol. 1957 Aug;41(8):461–474. doi: 10.1136/bjo.41.8.461. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Witkovsky P., Dudek F. E., Ripps H. Slow PIII component of the carp electroretinogram. J Gen Physiol. 1975 Feb;65(2):119–134. doi: 10.1085/jgp.65.2.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Witkovsky P., Nelson J., Ripps H. Action spectra and adaptation properties of carp photoreceptors. J Gen Physiol. 1973 Apr;61(4):401–423. doi: 10.1085/jgp.61.4.401. [DOI] [PMC free article] [PubMed] [Google Scholar]

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