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. 1982 Dec;152(3):1248–1254. doi: 10.1128/jb.152.3.1248-1254.1982

Volume growth, murein synthesis, and murein cross-linkage during the division cycle of Escherichia coli PA3092.

A J Olijhoek, S Klencke, E Pas, N Nanninga, U Schwarz
PMCID: PMC221633  PMID: 6754702

Abstract

Cells of Escherichia coli PA3092 were synchronized by centrifugal elutriation. The synchronously growing cells were double labeled with -3H or DL-[meso-2,6-14C]diaminopimelic acid (DAP) at different times. Cells incorporated [3H]DAP at a continuously increasing rate during their cycle, with a maximum occurring at about 30 min before division for trichloroacetic acid-precipitated cells (whole cells) and about 10 min before division for sodium dodecyl sulfate-treated cells (sacculi). This was in good agreement with the observed kinetics of volume growth under these conditions. Furazlocillin, which preferentially interacts with penicillin-binding protein 3, modified the pattern of incorporation of [3H]DAP. Electron microscopy indicated that furazlocillin did not inhibit the initiation of division but rather its completion. In addition, we measured the cross-linking of the murein inserted at different times during synchronous growth. The highest percentages were found to occur around division. At this same time, the cross-linking of old peptidoglycan was found to be decreased.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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