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. 1983 Feb;153(2):1075–1078. doi: 10.1128/jb.153.2.1075-1078.1983

In vivo packaging of cosmids in transposon-mediated mutagenesis.

F F White, H J Klee, E W Nester
PMCID: PMC221737  PMID: 6296041

Abstract

A technique was developed that permits the analysis of large regions of DNA by transposition mutagenesis. Large fragments of the pTiA6NC plasmid were cloned into the broad host range cosmid pHK17 and subjected to transposition mutagenesis by Tn3. Cosmids containing Tn3 insertions were selected by in vivo packaging by lambda cI857 and transduction to a new host. The insertions were localized by DNA restriction endonuclease analysis and transferred to the Ti-plasmid by marker exchange.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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