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. 1977 Oct;132(1):73–79. doi: 10.1128/jb.132.1.73-79.1977

Lipiarmycin-resistant ribonucleic acid polymerase mutants of Bacillus subtilis.

A L Sonenshein, H B Alexander, D M Rothstein, S H Fisher
PMCID: PMC221828  PMID: 410787

Abstract

Lipiarmycin inhibited the activity of deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase in vitro. We showed that inhibition was due to interference by lipiarmycin with the activity of sigma-containing molecules of RNA polymerase. Transcription by core enzyme was relatively resistant to the drug, but addition of sigma led to highly drug-sensitive RNA synthesis. We isolated lipiarmycin-resistant mutants of Bacillus subtilis and characterized them genetically and biochemically. Drug-resistant mutants contained an altered RNA polymerase that was resistant to the drug in vitro. By separation and mixed reconstitution of core and sigma fractions of mutant and wild-type RNA polymerase, we showed that lipiarmycin resistance in one mutant strain was a property of the core fraction. Genetic mapping experiments indicated that at least two lpm mutants are located between loci determining rifampin resistance and streptolydigin resistance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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