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. 1977 Oct;132(1):247–253. doi: 10.1128/jb.132.1.247-253.1977

Immunological study of the regulation of cellular arylsulfatase synthesis in Klebsiella aerogenes.

Y Murooka, T Yamada, S Tanabe, T Harada
PMCID: PMC221850  PMID: 72063

Abstract

Regulation of cellular arylsulfatase synthesis in Klebsiella aerogenes was analyzed by immunological techniques. Antibody directed against the purified arylsulfatase from K. aerogenes W70 was obtained from rabbits and characterized by immunoelectrophoresis, double-diffusion, quantitative precipitation, and enzyme neutralization tests. Arylsulfatase was located in the periplasmic space when the wild-type strain was cultured with methionine or with inorganic sulfate plus tyramine, but not with inorganic sulfate without tyramine, as the sole sulfur source. Tyramine oxidase was retained in the membrane fraction prepared from cells grown in the presence of tyramine. Arylsulfatase protein was not synthesized in the presence of tyramine and inorganic sulfate by mutant K611, which is deficient in tyramine oxidase (tynA). We conclude that the expression of the arylsulfatase gene (atsA) is regulated by the expression of tynA and that inorganic sulfate serves as a corepressor. In addition, strains mutated in the atsA gene were analyzed by using antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adachi T., Murooka Y., Harada T. Derepression of arylsulfatase synthesis in Aerobacter aerogenes by tyramine. J Bacteriol. 1973 Oct;116(1):19–24. doi: 10.1128/jb.116.1.19-24.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adachi T., Murooka Y., Harada T. Regulation of arylsulfatase synthesis by sulfur compounds in Klebsiella aerogenes. J Bacteriol. 1975 Jan;121(1):29–35. doi: 10.1128/jb.121.1.29-35.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Allen E., Roy A. B. The sulphatase of ox liver. XI. The isoelectric focussing of a purified preparation of sulphatase B. Biochim Biophys Acta. 1968 Oct 21;168(2):243–251. doi: 10.1016/0005-2795(68)90147-5. [DOI] [PubMed] [Google Scholar]
  4. Fitzgerald J. W. Sulfate ester formation and hydrolysis: a potentially important yet often ignored aspect of the sulfur cycle of aerobic soils. Bacteriol Rev. 1976 Sep;40(3):698–721. doi: 10.1128/br.40.3.698-721.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  6. London J., Meyer E. Y., Kulczyk S. Comparative biochemical and immunological study of malic enzyme from two species of lactic acid bacteria: evolutionary implications. J Bacteriol. 1971 Apr;106(1):126–137. doi: 10.1128/jb.106.1.126-137.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Morris H., Schlesinger M. J., Bracha M., Yagil E. Pleiotropic effects of mutations involved in the regulation of Escherichia coli K-12 alkaline phosphatase. J Bacteriol. 1974 Aug;119(2):583–592. doi: 10.1128/jb.119.2.583-592.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Murooka Y., Adachi T., Okamura H., Harada T. Genetic control of arylsulfatase synthesis in Klebsiella aerogenes. J Bacteriol. 1977 Apr;130(1):74–81. doi: 10.1128/jb.130.1.74-81.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. NICHOL L. W., ROY A. B. THE SULPHATASE OF OX LIVER. 8. THE SEDIMENTATION OF PURIFIED SULPHATASE A. J Biochem. 1964 Jun;55:643–651. doi: 10.1093/oxfordjournals.jbchem.a127938. [DOI] [PubMed] [Google Scholar]
  10. OUCHTERLONY O. Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta Pathol Microbiol Scand. 1953;32(2):230–240. [PubMed] [Google Scholar]
  11. Okamura H., Murooka Y., Harada T. Regulation of tyramine oxidase synthesis in Klebsiella aerogenes. J Bacteriol. 1976 Jul;127(1):24–31. doi: 10.1128/jb.127.1.24-31.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Okamura H., Murooka Y., Harada T. Tyramine oxidase and regulation of arylsulfatase synthesis in Klebsiella aerogenes. J Bacteriol. 1977 Jan;129(1):59–65. doi: 10.1128/jb.129.1.59-65.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schlesinger M. J. Secretion of alkaline phosphatase subunits by spheroplasts of Escherichia coli. J Bacteriol. 1968 Sep;96(3):727–733. doi: 10.1128/jb.96.3.727-733.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. TORRIANI A. Influence of inorganic phosphate in the formation of phosphatases by Escherichia coli. Biochim Biophys Acta. 1960 Mar 11;38:460–469. doi: 10.1016/0006-3002(60)91281-6. [DOI] [PubMed] [Google Scholar]
  15. Willsky G. R., Malamy M. H. Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli. J Bacteriol. 1976 Jul;127(1):595–609. doi: 10.1128/jb.127.1.595-609.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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