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. 1977 Oct;132(1):332–340. doi: 10.1128/jb.132.1.332-340.1977

Studies of colicin E1 plasmid functions by analysis of deletions and TnA insertions of the plasmid.

J Inselburg
PMCID: PMC221860  PMID: 334729

Abstract

The further identification of regions of the colicin E1 plasmid that affect plasmid functions has been achieved by studying deletions and TnA insertions of the plasmid. Colicin production, colicin immunity, relaxation of plasmid deoxyribonucleic acid, and plasmid incompatibility functions have been examined. A strong correlation has been observed between the ability of colicin E1 plasmid deoxyribonucleic acid to be relaxed and the ability of that plasmid to be transferred by conjugation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonhoeffer F., Vielmetter W. Conjugational DNA transfer in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1968;33:623–627. doi: 10.1101/sqb.1968.033.01.069. [DOI] [PubMed] [Google Scholar]
  2. Clewell D. B., Helinski D. R. Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol. 1972 Jun;110(3):1135–1146. doi: 10.1128/jb.110.3.1135-1146.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clewell D. B., Helinski D. R. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970 Oct 27;9(22):4428–4440. doi: 10.1021/bi00824a026. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cohen S. N., Chang A. C., Hsu L. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110–2114. doi: 10.1073/pnas.69.8.2110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fuke M., Inselburg J. Electron microscopic studies of replicating and catenated colicin factor E1 DNA isolated from minicells (DNA replication). Proc Natl Acad Sci U S A. 1972 Jan;69(1):89–92. doi: 10.1073/pnas.69.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gilbert W., Dressler D. DNA replication: the rolling circle model. Cold Spring Harb Symp Quant Biol. 1968;33:473–484. doi: 10.1101/sqb.1968.033.01.055. [DOI] [PubMed] [Google Scholar]
  9. Hershfield V., Boyer H. W., Yanofsky C., Lovett M. A., Helinski D. R. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455–3459. doi: 10.1073/pnas.71.9.3455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Inselburg J., Fuke M. Isolation of catenated and replicating DNA molecules of colicin factor E1 from minicells. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2839–2842. doi: 10.1073/pnas.68.11.2839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Inselburg J. Incompatibility exhibited by colicin plasmids E1, E2, and E3 in Escherichia coli. J Bacteriol. 1974 Aug;119(2):478–483. doi: 10.1128/jb.119.2.478-483.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Inselburg J. Isolation, mapping, and examination of effects of TnA insertions in ColE1 plasmids. J Bacteriol. 1977 Jan;129(1):482–491. doi: 10.1128/jb.129.1.482-491.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Inselburg J. Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256–2259. doi: 10.1073/pnas.71.6.2256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Inselburg J., Ware P. Isolation and genetic analysis of deletion mutants of colicin E1 plasmids carrying a TnA insertion. J Bacteriol. 1977 Oct;132(1):321–331. doi: 10.1128/jb.132.1.321-331.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lovett M. A., Guiney D. G., Helinski D. R. Relaxation complexes of plasmids ColE1 and ColE2: unique site of the nick in the open circular DNA of the relaxed complexes. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3854–3857. doi: 10.1073/pnas.71.10.3854. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lovett M. A., Katz L., Helinski D. R. Unidirectional replication of plasmid ColE1 DNA. Nature. 1974 Sep 27;251(5473):337–340. doi: 10.1038/251337a0. [DOI] [PubMed] [Google Scholar]
  17. Ohki M., Tomizawa J. Asymmetric transfer of DNA strands in bacterial conjugation. Cold Spring Harb Symp Quant Biol. 1968;33:651–658. doi: 10.1101/sqb.1968.033.01.074. [DOI] [PubMed] [Google Scholar]
  18. Oka A., Takanami M. Cleavage map of colicin E1 plasmid. Nature. 1976 Nov 11;264(5582):193–196. doi: 10.1038/264193a0. [DOI] [PubMed] [Google Scholar]
  19. So M., Gill R., Falkow S. The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA. Mol Gen Genet. 1975 Dec 30;142(3):239–249. doi: 10.1007/BF00425649. [DOI] [PubMed] [Google Scholar]
  20. Sugino Y., Tomizawa J., Kakefuda T. Location of non-DNA components of closed circular colicin E1 plasmid DNA. Nature. 1975 Feb 20;253(5493):652–654. doi: 10.1038/253652a0. [DOI] [PubMed] [Google Scholar]
  21. Tomizawa J., Sakakibara Y., Kakefuda T. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260–2264. doi: 10.1073/pnas.71.6.2260. [DOI] [PMC free article] [PubMed] [Google Scholar]

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