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. 1978 Feb;133(2):447–451. doi: 10.1128/jb.133.2.447-451.1978

Regulation of gamma-aminobutyric acid degradation in Escherichia coli by nitrogen metabolism enzymes.

M Zaboura, Y S Halpern
PMCID: PMC222043  PMID: 24037

Abstract

The possible role of glutamate dehydrogenase, glutamate synthase, and glutamine synthetase in the regulation of enzyme formation in the gamma-aminobutyric acid (GABA) catabolic pathway of Escherichia coli K-12 was investigated. Evidence is presented indicating that glutamine synthetase acts as a positive regulator in the E. coli GABA control system. Mutations impairing glutamate synthase activity prevent the depression of the enzymes of the GABA pathway in ammonia-limited glucose media. However, mutations resulting in constitutive synthesis of glutamine synthetase (GlnC) restore the ability of the glutamate synthase-less mutants to grow in glucose-GABA media and result in depressed synthesis of the GABA enzymes. It is suggested that the loss of glutamate synthesis activity affects the GABA control system indirectly by lowering glutamine synthetase levels.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bender R. A., Janssen K. A., Resnick A. D., Blumenberg M., Foor F., Magasanik B. Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. J Bacteriol. 1977 Feb;129(2):1001–1009. doi: 10.1128/jb.129.2.1001-1009.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berberich M. A. A glutamate-dependent phenotype in E. coli K12: the result of two mutations. Biochem Biophys Res Commun. 1972 Jun 28;47(6):1498–1503. doi: 10.1016/0006-291x(72)90242-2. [DOI] [PubMed] [Google Scholar]
  4. Brenchley J. E., Prival M. J., Magasanik B. Regulation of the synthesis of enzymes responsible for glutamate formation in Klebsiella aerogenes. J Biol Chem. 1973 Sep 10;248(17):6122–6128. [PubMed] [Google Scholar]
  5. DAVIS B. D., MINGIOLI E. S. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. doi: 10.1128/jb.60.1.17-28.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dover S., Halpern Y. S. Control of the pathway of -aminobutyrate breakdown in Escherichia coli K-12. J Bacteriol. 1972 Apr;110(1):165–170. doi: 10.1128/jb.110.1.165-170.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dover S., Halpern Y. S. Genetic analysis of the gamma-aminobutyrate utilization pathway in Escherichia coli K-12. J Bacteriol. 1974 Feb;117(2):494–501. doi: 10.1128/jb.117.2.494-501.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldberg R. B., Bloom F. R., Magasanik B. Regulation of histidase synthesis in intergeneric hybrids of enteric bacteria. J Bacteriol. 1976 Jul;127(1):114–119. doi: 10.1128/jb.127.1.114-119.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Low B. Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. J Bacteriol. 1973 Feb;113(2):798–812. doi: 10.1128/jb.113.2.798-812.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mayer E. P., Smith O. H., Fredricks W. W., McKinney M. A. The isolation and characterization of glutamine-requiring strains of Escherichia coli K12. Mol Gen Genet. 1975;137(2):131–142. doi: 10.1007/BF00341679. [DOI] [PubMed] [Google Scholar]
  13. NEIDHARDT F. C., MAGASANIK B. Reversal of the glucose inhibition of histidase biosynthesis in Aerobacter aerogenes. J Bacteriol. 1957 Feb;73(2):253–259. doi: 10.1128/jb.73.2.253-259.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Newman B. M., Cole J. A. Lack of a regulatory function for glutamine synthetase protein in the synthesis of glutamate dehydrogenase and nitrite reductase in Escherichia coli K12. J Gen Microbiol. 1977 Feb;98(2):369–377. doi: 10.1099/00221287-98-2-369. [DOI] [PubMed] [Google Scholar]
  15. Pahel G., Zelenetz A. D., Tyler B. M. gltB gene and regulation of nitrogen metabolism by glutamine synthetase in Escherichia coli. J Bacteriol. 1978 Jan;133(1):139–148. doi: 10.1128/jb.133.1.139-148.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Prival M. J., Brenchley J. E., Magasanik B. Glutamine synthetase and the regulation of histidase formation in Klebsiella aerogenes. J Biol Chem. 1973 Jun 25;248(12):4334–4344. [PubMed] [Google Scholar]
  17. Resnick A. D., Magasanik B. L-Asparaginase of Klebsiella aerogenes. Activation of its synthesis by glutamine synthetase. J Biol Chem. 1976 May 10;251(9):2722–2728. [PubMed] [Google Scholar]
  18. Streicher S. L., Deleo A. B., Magasanik B. Regulation of enzyme formation in Klebsiella aerogenes by episomal glutamine synthetase of Escherichia coli. J Bacteriol. 1976 Jul;127(1):184–192. doi: 10.1128/jb.127.1.184-192.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Streicher S. L., Shanmugam K. T., Ausubel F., Morandi C., Goldberg R. B. Regulation of nitrogen fixation in Klebsiella pneumoniae: evidence for a role of glutamine synthetase as a regulator of nitrogenase synthesis. J Bacteriol. 1974 Nov;120(2):815–821. doi: 10.1128/jb.120.2.815-821.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tyler B., Deleo A. B., Magasanik B. Activation of transcription of hut DNA by glutamine synthetase. Proc Natl Acad Sci U S A. 1974 Jan;71(1):225–229. doi: 10.1073/pnas.71.1.225. [DOI] [PMC free article] [PubMed] [Google Scholar]

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