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. 1978 Feb;133(2):527–535. doi: 10.1128/jb.133.2.527-535.1978

TOL plasmid in Pseudomonas aeruginosa PAO: thermosensitivity of self-maintenance and inhibition of host cell growth.

T Nakazawa
PMCID: PMC222054  PMID: 415040

Abstract

The TOL plasmid originally isolated in Pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of Pseudomonas, i.e., P. putida, P. fluorescens, and P. aeruginosa, except for a strain of P. aeruginosa, strain PAO. The same strain, however, could accept the plasmid when its restriction and modification abilities were lost by mutations or by growing at high temperature. In addition, the transmissibility of the TOL plasmid from strain PAO to P. putida was low when the plasmid was modified by the donor. By using P. aeruginosa PAO carrying the TOL plasmid, the stability and genetic expression of the plasmid as well as its effect on the host cell growth were examined. Thus the self-maintenance of the plasmid was found to be thermosensitive. Furthermore, the TOL plasmid inhibited the growth of strain PAO at high temperature, accompanied by the formation of some filamentous cells. These thermosensitive properties of the TOL plasmid were host dependent and not exhibited in another strain of P. aeruginosa.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chakrabarty A. M. Genetic basis of the biodegradation of salicylate in Pseudomonas. J Bacteriol. 1972 Nov;112(2):815–823. doi: 10.1128/jb.112.2.815-823.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chakrabarty A. M. Plasmids in Pseudomonas. Annu Rev Genet. 1976;10:7–30. doi: 10.1146/annurev.ge.10.120176.000255. [DOI] [PubMed] [Google Scholar]
  3. Chang B. J., Holloway B. W. Bacterial mutation affecting plasmid maintenance in Pseudomonas aeruginosa. J Bacteriol. 1977 May;130(2):943–945. doi: 10.1128/jb.130.2.943-945.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. Properties of an R factor from Pseudomonas aeruginosa. J Bacteriol. 1971 Dec;108(3):1244–1249. doi: 10.1128/jb.108.3.1244-1249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966 Jul;54(1):61–76. doi: 10.1093/genetics/54.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DiJoseph C. G., Bayer M. E., Kaji A. Host cell growth in the presence of the thermosensitive drug resistance factor, Rts1. J Bacteriol. 1973 Jul;115(1):399–410. doi: 10.1128/jb.115.1.399-410.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dunn N. W., Holloway B. W. Pleiotrophy of p-fluorophenylalanine-resistant and antibiotic hypersensitive mutants of Pseudomonas aeruginosa. Genet Res. 1971 Oct;18(2):185–197. doi: 10.1017/s0016672300012593. [DOI] [PubMed] [Google Scholar]
  8. HOLLOWAY B. W. VARIATIONS IN RESTRICTION AND MODIFICATION OF BACTERIOPHAGE FOLLOWING INCREASE OF GROWTH TEMPERATURE OF PSEUDOMONAS AERUGINOSA. Virology. 1965 Apr;25:634–642. doi: 10.1016/0042-6822(65)90091-7. [DOI] [PubMed] [Google Scholar]
  9. Kingsbury D. T., Sieckmann D. G., Helinski D. R. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli. II. Properties of host and plasmid mutations. Genetics. 1973 May;74(1):1–16. doi: 10.1093/genetics/74.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Koyama A. H., Yura T. Plasmid mutations affecting self-maintenance and host growth in Escherichia coli. J Bacteriol. 1975 Apr;122(1):80–88. doi: 10.1128/jb.122.1.80-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Matsumoto H., Tazaki T. FP5 factor, an undescribed sex factor of Pseudomonas aeruginosa. Jpn J Microbiol. 1973 Sep;17(5):409–417. doi: 10.1111/j.1348-0421.1973.tb00792.x. [DOI] [PubMed] [Google Scholar]
  12. Nakazawa T., Hayashi E. Phthalate metabolism in Pseudomonas testosteroni: accumulation of 4,5-dihydroxyphthalate by a mutant strain. J Bacteriol. 1977 Jul;131(1):42–48. doi: 10.1128/jb.131.1.42-48.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nakazawa T., Yokota T. Isolation of a mutant TOL plasmid with increased activity and transmissibility from Pseudomonas putida (arvilla) mt-2. J Bacteriol. 1977 Jan;129(1):39–46. doi: 10.1128/jb.129.1.39-46.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pemberton J. M., Holloway B. W. Chromosome mapping in Pseudomonas aeruginosa. Genet Res. 1972 Jun;19(3):251–260. doi: 10.1017/s0016672300014518. [DOI] [PubMed] [Google Scholar]
  15. Rheinwald J. G., Chakrabarty A. M., Gunsalus I. C. A transmissible plasmid controlling camphor oxidation in Pseudomonas putida. Proc Natl Acad Sci U S A. 1973 Mar;70(3):885–889. doi: 10.1073/pnas.70.3.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rolfe B., Holloway B. W. Genetic control of DNA specificity in Pseudomonas aeruginosa. Genet Res. 1968 Aug;12(1):99–102. doi: 10.1017/s0016672300011678. [DOI] [PubMed] [Google Scholar]
  17. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  18. Terawaki Y., Ishizu K., Horiuchi S., Goto N., Nakaya R. Control of replication and segregation of R plasmid Rts1. J Bacteriol. 1976 Dec;128(3):693–700. doi: 10.1128/jb.128.3.693-700.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Terawaki Y., Kakizawa Y., Takayasu H., Yoshikawa M. Temperature sensitivity of cell growth in Escherichia coli associated with the temperature sensitive R(KM) factor. Nature. 1968 Jul 20;219(5151):284–285. doi: 10.1038/219284a0. [DOI] [PubMed] [Google Scholar]
  20. Terawaki Y., Kishi H., Nakaya R. Integration of R plasmid Rts1 to the gal region of the Escherichia coli chromosome. J Bacteriol. 1975 Mar;121(3):857–862. doi: 10.1128/jb.121.3.857-862.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Terawaki Y., Takayasu H., Akiba T. Thermosensitive replication of a kanamycin resistance factor. J Bacteriol. 1967 Sep;94(3):687–690. doi: 10.1128/jb.94.3.687-690.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Williams P. A., Murray K. Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. J Bacteriol. 1974 Oct;120(1):416–423. doi: 10.1128/jb.120.1.416-423.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wong C. L., Dunn N. W. Transmissible plasmid coding for the degradation of benzoate and m-toluate in Pseudomonas arvilla mt-2. Genet Res. 1974 Apr;23(2):227–232. doi: 10.1017/s0016672300014853. [DOI] [PubMed] [Google Scholar]
  24. Worsey M. J., Williams P. A. Metabolism of toluene and xylenes by Pseudomonas (putida (arvilla) mt-2: evidence for a new function of the TOL plasmid. J Bacteriol. 1975 Oct;124(1):7–13. doi: 10.1128/jb.124.1.7-13.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yoshimoto H., Yoshikawa M. Chromosome-plasmid interaction in Escherichia coli K-12 carrying a thermosensitive plasmid, Rts1, in autonomous and in integrated states. J Bacteriol. 1975 Nov;124(2):661–667. doi: 10.1128/jb.124.2.661-667.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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