Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Feb;133(2):699–707. doi: 10.1128/jb.133.2.699-707.1978

Transduction of plasmid determinants in Staphylococcus aureus and Escherichia coli.

M H Ubelaker, E D Rosenblum
PMCID: PMC222078  PMID: 342503

Abstract

Buoyant density analysis of transducing lysates derived from Staphylococcus aureus and Escherichia coli indicated that phage particles bearing plasmid determinants contain a quantity of DNA equivalent to that found in the lytic particles. Transducing particles that bear plasmid determinants smaller than viral DNA must therefore contain a quantity of DNA in excess of a single plasmid genome. In the E. coli P1vir system, a dependence upon host-mediated recombination for the transduction of small plasmids, but not for large R factors or chromosomal genes, was observed. However, no evidence for the involvement of such functions in the transduction of S. aureus plasmids was obtained. Although the origin of the additional DNA in plasmid transducing particles has not been identified, circumstantial evidence has been presented in the staphylococcal system indicating that transducing particles carrying a small tetracycline plasmid are not formed by the wrapping of multiple copies of this plasmid DNA.

Full text

PDF
707

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARBER W. Transduction of chromosomal genes and episomes in Escherichia coli. Virology. 1960 May;11:273–288. doi: 10.1016/0042-6822(60)90066-0. [DOI] [PubMed] [Google Scholar]
  2. BLAIR J. E., BYNOE E. T. Variation in three staphylococcal typing phages. Public Health Rep. 1958 May;73(5):465–466. [PMC free article] [PubMed] [Google Scholar]
  3. BLAIR J. E., CARR M. The bacteriophage typing of staphylococci. J Infect Dis. 1953 Jul-Aug;93(1):1–13. doi: 10.1093/infdis/93.1.1. [DOI] [PubMed] [Google Scholar]
  4. Berryhill D. L., Pattee P. A. Buoyant density analysis of staphylococcal bacteriophage 80 transducing particles. J Virol. 1969 Nov;4(5):804–806. doi: 10.1128/jvi.4.5.804-806.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Botstein D., Matz M. J. A recombination function essential to the growth of bacteriophage P22. J Mol Biol. 1970 Dec 28;54(3):417–440. doi: 10.1016/0022-2836(70)90119-1. [DOI] [PubMed] [Google Scholar]
  6. Chan R. K., Botstein D., Watanabe T., Ogata Y. Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate. Virology. 1972 Dec;50(3):883–898. doi: 10.1016/0042-6822(72)90442-4. [DOI] [PubMed] [Google Scholar]
  7. DUBNAU E., STOCKER B. A. GENETICS OF PLASMIDS IN SALMONELLA TYPHIMURIUM. Nature. 1964 Dec 12;204:1112–1113. doi: 10.1038/2041112a0. [DOI] [PubMed] [Google Scholar]
  8. Dowell C. E., Rosenblum E. D. STAPHYLOCOCCAL TRANSDUCING PARTICLE. J Bacteriol. 1962 Nov;84(5):1076–1079. doi: 10.1128/jb.84.5.1076-1079.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ebel-Tsipis J., Botstein D., Fox M. S. Generalized transduction by phage P22 in Salmonella typhimurium. I. Molecular origin of transducing DNA. J Mol Biol. 1972 Nov 14;71(2):433–448. doi: 10.1016/0022-2836(72)90361-0. [DOI] [PubMed] [Google Scholar]
  10. Grubb W. B., O'Reilly R. J. Joint transduction of separate extrachromosomal drug resistance determinants in Staphylococcus aureus E169. Biochem Biophys Res Commun. 1971 Feb 5;42(3):377–383. doi: 10.1016/0006-291x(71)90381-0. [DOI] [PubMed] [Google Scholar]
  11. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ikeda H., Tomizawa J. I. Transducing fragments in generalized transduction by phage P1. I. Molecular origin of the fragments. J Mol Biol. 1965 Nov;14(1):85–109. doi: 10.1016/s0022-2836(65)80232-7. [DOI] [PubMed] [Google Scholar]
  13. Ikeda H., Tomizawa J. Prophage P1, and extrachromosomal replication unit. Cold Spring Harb Symp Quant Biol. 1968;33:791–798. doi: 10.1101/sqb.1968.033.01.091. [DOI] [PubMed] [Google Scholar]
  14. KONDO E., MITSUHASHI S. DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1. J Bacteriol. 1964 Nov;88:1266–1276. doi: 10.1128/jb.88.5.1266-1276.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kameda M., Harada K., Suzuki M., Mitsuhashi S. Drug resistance of enteric bacteria. V. High frequency of transduction of R factors with bacteriophage epsilon. J Bacteriol. 1965 Nov;90(5):1174–1181. doi: 10.1128/jb.90.5.1174-1181.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kasuga T., Mitsuhashi S. Drug resistance of staphylococci. 8. Genetic properties of resistance to tetracycline in Staphylococcus aureus E169. Jpn J Microbiol. 1968 Sep;12(3):269–273. doi: 10.1111/j.1348-0421.1968.tb00395.x. [DOI] [PubMed] [Google Scholar]
  17. Kontomichalou P., Mitani M., Clowes R. C. Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control. J Bacteriol. 1970 Oct;104(1):34–44. doi: 10.1128/jb.104.1.34-44.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. LENNOX E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology. 1955 Jul;1(2):190–206. doi: 10.1016/0042-6822(55)90016-7. [DOI] [PubMed] [Google Scholar]
  19. Nisioka T., Mitani M., Clowes R. C. Molecular recombination between R-factor deoxyribonucleic acid molecules in Escherichia coli host cells. J Bacteriol. 1970 Jul;103(1):166–177. doi: 10.1128/jb.103.1.166-177.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Novick R. P. Extrachromosomal inheritance in bacteria. Bacteriol Rev. 1969 Jun;33(2):210–263. doi: 10.1128/br.33.2.210-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Novick R. P., Roth C. Plasmid-linked resistance to inorganic salts in Staphylococcus aureus. J Bacteriol. 1968 Apr;95(4):1335–1342. doi: 10.1128/jb.95.4.1335-1342.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Novick R. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus. Virology. 1967 Sep;33(1):155–166. doi: 10.1016/0042-6822(67)90105-5. [DOI] [PubMed] [Google Scholar]
  23. Pariza M. W., Iandolo J. J. Determination of genome size of selected typing bacteriophages of Staphylococcus aureus. Appl Microbiol. 1974 Sep;28(3):510–512. doi: 10.1128/am.28.3.510-512.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Rubin S. J., Rosenblum E. D. Effects of the recipient strain and ultraviolet irradiation on transduction kinetics of the penicillinase plasmid of Staphylococcus aureus. J Bacteriol. 1971 Dec;108(3):1192–1199. doi: 10.1128/jb.108.3.1192-1199.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. SHEPPARD D. E. Density gradient centrifugation of bacteriophage P22. Virology. 1962 May;17:212–214. doi: 10.1016/0042-6822(62)90105-8. [DOI] [PubMed] [Google Scholar]
  26. SWANSTROM M., ADAMS M. H. Agar layer method for production of high titer phage stocks. Proc Soc Exp Biol Med. 1951 Nov;78(2):372–375. doi: 10.3181/00379727-78-19076. [DOI] [PubMed] [Google Scholar]
  27. Schmieger H. Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet. 1972;119(1):75–88. doi: 10.1007/BF00270447. [DOI] [PubMed] [Google Scholar]
  28. Schmieger H. The molecular structure of the transducing particles of Salmonella phage P22. II. Density gradient analysis of DNA. Mol Gen Genet. 1970;109(4):323–337. doi: 10.1007/BF00267702. [DOI] [PubMed] [Google Scholar]
  29. Signer E. R., Weil J. Recombination in bacteriophage lambda. I. Mutants deficient in general recombination. J Mol Biol. 1968 Jul 14;34(2):261–271. doi: 10.1016/0022-2836(68)90251-9. [DOI] [PubMed] [Google Scholar]
  30. Stiffler P. W., Sweeney H. M., Cohen S. Co-transduction of plasmids mediating resistance to tetracycline and chloramphenicol in Staphylococcus aureus. J Bacteriol. 1974 Nov;120(2):934–934. doi: 10.1128/jb.120.2.934-944.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. TING R. C. The specific gravity of transducing particles of bacteriophage P1. Virology. 1962 Feb;16:115–121. doi: 10.1016/0042-6822(62)90286-6. [DOI] [PubMed] [Google Scholar]
  32. Ubelaker M. H., Rosenblum E. D. DNA replication in bacteriophage-infected Staphylococcus aureus. J Virol. 1977 Dec;24(3):768–774. doi: 10.1128/jvi.24.3.768-774.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Wall J. D., Harriman P. D. Phage P1 mutants with altered transducing abilities for Escherichia coli. Virology. 1974 Jun;59(2):532–544. doi: 10.1016/0042-6822(74)90463-2. [DOI] [PubMed] [Google Scholar]
  34. Wyman L., Goering R. V., Novick R. P. Genetic control of chromosomal and plasmid recombination in Staphylococcus aureus. Genetics. 1974 Apr;76(4):681–702. doi: 10.1093/genetics/76.4.681. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES