Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1978 Mar;133(3):1287–1294. doi: 10.1128/jb.133.3.1287-1294.1978

Replication of colicin E1 plasmid DNA in vivo requires no plasmid-encoded proteins.

D J Donoghue, P A Sharp
PMCID: PMC222164  PMID: 346567

Abstract

A derivative of bacteriophage lambda containing a colicin E1 plasmid replicon was constructed by recombinant DNA techniques. This phage, lambdacol100, has two functional modes of DNA replication; it can replicate via either plasmid or phage replication systems. lambdacol100 has been used to introduce the colicin E1 plasmid replicon into Escherichia coli previously treated with chloramphenicol to block protein synthesis. Under these conditions, lambdacol100 DNA is replicated normally as a colicin E1 plasmid. This suggests that colicin E1 plasmid replication in vivo does not require any plasmid-encoded proteins.

Full text

PDF
1287

Images in this article

1290Image
on p.1290
1292Image
on p.1292

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amati P. Chloramphenical: effect on DNA synthesis during phage development in Escherichia coli. Science. 1970 Jun 5;168(3936):1226–1228. doi: 10.1126/science.168.3936.1226. [DOI] [PubMed] [Google Scholar]
  2. Avni H., Berg P. E., Markovitz A. New mini-ColE1 as a molecular cloning vehicle. J Bacteriol. 1977 Jan;129(1):358–366. doi: 10.1128/jb.129.1.358-366.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bazaral M., Helinski D. R. Replication of a bacterial plasmid and an episome in Escherichia coli. Biochemistry. 1970 Jan 20;9(2):399–406. doi: 10.1021/bi00804a029. [DOI] [PubMed] [Google Scholar]
  5. Blair D. G., Sherratt D. J., Clewell D. B., Helinski D. R. Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2518–2522. doi: 10.1073/pnas.69.9.2518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cabello F., Timmis K., Cohen S. N. Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature. 1976 Jan 29;259(5541):285–290. doi: 10.1038/259285a0. [DOI] [PubMed] [Google Scholar]
  7. Clewell D. B. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. doi: 10.1128/jb.110.2.667-676.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Donoghue D. J., Sharp P. A. An improved bacteriophage lambda vector: construction of model recombinants coding for kanamycin resistance. Gene. 1977 May;1(3-4):209–227. doi: 10.1016/0378-1119(77)90046-4. [DOI] [PubMed] [Google Scholar]
  9. Hershfield V., Boyer H. W., Chow L., Helinski D. R. Characterization of a mini-ColC1 plasmid. J Bacteriol. 1976 Apr;126(1):447–453. doi: 10.1128/jb.126.1.447-453.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hershfield V., Boyer H. W., Yanofsky C., Lovett M. A., Helinski D. R. Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3455–3459. doi: 10.1073/pnas.71.9.3455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Imamoto F., Tani S. Diversity of regulation of genetic transcription. Nat New Biol. 1972 Dec 6;240(101):172–175. doi: 10.1038/newbio240172a0. [DOI] [PubMed] [Google Scholar]
  12. Inselburg J. Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256–2259. doi: 10.1073/pnas.71.6.2256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kingsbury D. T., Helinski D. R. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli. I. Isolation and specificity of host and plasmid mutations. Genetics. 1973 May;74(1):17–31. doi: 10.1093/genetics/74.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kingsbury D. T., Sieckmann D. G., Helinski D. R. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli. II. Properties of host and plasmid mutations. Genetics. 1973 May;74(1):1–16. doi: 10.1093/genetics/74.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  16. Lieb M. Lambda mutants which persist as plasmids. J Virol. 1970 Aug;6(2):218–225. doi: 10.1128/jvi.6.2.218-225.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lovett M. A., Helinski D. R. Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid. J Bacteriol. 1976 Aug;127(2):982–987. doi: 10.1128/jb.127.2.982-987.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lovett M. A., Katz L., Helinski D. R. Unidirectional replication of plasmid ColE1 DNA. Nature. 1974 Sep 27;251(5473):337–340. doi: 10.1038/251337a0. [DOI] [PubMed] [Google Scholar]
  19. Matsubara K., Kaiser A. D. Lambda dv: an autonomously replicating DNA fragment. Cold Spring Harb Symp Quant Biol. 1968;33:769–775. doi: 10.1101/sqb.1968.033.01.088. [DOI] [PubMed] [Google Scholar]
  20. Ohtsubo H., Ohtsubo E. Isolation of inverted repeat sequences, including IS1, IS2, and IS3, in Escherichia coli plasmids. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2316–2320. doi: 10.1073/pnas.73.7.2316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pestka S. Inhibitors of ribosome functions. Annu Rev Microbiol. 1971;25:487–562. doi: 10.1146/annurev.mi.25.100171.002415. [DOI] [PubMed] [Google Scholar]
  22. Sakakibara Y., Tomizawa J. I. Replication of colicin E1 plasmid DNA in cell extracts. Proc Natl Acad Sci U S A. 1974 Mar;71(3):802–806. doi: 10.1073/pnas.71.3.802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  24. Signer E. R. Plasmid formation: a new mode of lysogeny by phase lambda. Nature. 1969 Jul 12;223(5202):158–160. doi: 10.1038/223158a0. [DOI] [PubMed] [Google Scholar]
  25. Signer E. R., Weil J. Recombination in bacteriophage lambda. I. Mutants deficient in general recombination. J Mol Biol. 1968 Jul 14;34(2):261–271. doi: 10.1016/0022-2836(68)90251-9. [DOI] [PubMed] [Google Scholar]
  26. THOMAS R. Effects of chloramphenicol on genetic replication in bacteriophage lambda. Virology. 1959 Oct;9:275–289. doi: 10.1016/0042-6822(59)90120-5. [DOI] [PubMed] [Google Scholar]
  27. Thomas M., Cameron J. R., Davis R. W. Viable molecular hybrids of bacteriophage lambda and eukaryotic DNA. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4579–4583. doi: 10.1073/pnas.71.11.4579. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tomizawa J. I., Sakakibara Y., Kakefuda T. Replication of colicin E1 plasmid DNA added to cell extracts. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1050–1054. doi: 10.1073/pnas.72.3.1050. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Tomizawa J., Sakakibara Y., Kakefuda T. Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260–2264. doi: 10.1073/pnas.71.6.2260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. WISSEMAN C. L., Jr, SMADEL J. E., HAHN F. E., HOPPS H. E. Mode of action of chloramphenicol. I. Action of chloramphenicol on assimilation of ammonia and on synthesis of proteins and nucleic acids in Escherichia coli. J Bacteriol. 1954 Jun;67(6):662–673. doi: 10.1128/jb.67.6.662-673.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Womble D. D., Taylor D. P., Rownd R. H. Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation. J Bacteriol. 1977 Apr;130(1):148–153. doi: 10.1128/jb.130.1.148-153.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Young E. T., 2nd, Sinsheimer R. L. Vegetative bacteriophage lambda-DNA. II. Physical characterization and replication. J Mol Biol. 1967 Nov 28;30(1):165–200. doi: 10.1016/0022-2836(67)90251-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES